Citation
Nyc, Otakar, et al. "Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium Difficile PCR Ribotype 001 Strain Recognized By Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis." Microbial Drug Resistance (Larchmont, N.Y.), vol. 23, no. 5, 2017, pp. 609-615.
Nyc O, Tejkalova R, Kriz Z, et al. Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis. Microb Drug Resist. 2017;23(5):609-615.
Nyc, O., Tejkalova, R., Kriz, Z., Ruzicka, F., Kubicek, L., Matejkova, J., Kuijper, E., & Krutova, M. (2017). Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis. Microbial Drug Resistance (Larchmont, N.Y.), 23(5), 609-615. https://doi.org/10.1089/mdr.2016.0159
Nyc O, et al. Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium Difficile PCR Ribotype 001 Strain Recognized By Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis. Microb Drug Resist. 2017;23(5):609-615. PubMed PMID: 27860531.
TY - JOUR
T1 - Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis.
AU - Nyc,Otakar,
AU - Tejkalova,Renata,
AU - Kriz,Zdenek,
AU - Ruzicka,Filip,
AU - Kubicek,Lubos,
AU - Matejkova,Jana,
AU - Kuijper,Ed,
AU - Krutova,Marcela,
Y1 - 2016/11/18/
PY - 2016/11/20/pubmed
PY - 2018/3/31/medline
PY - 2016/11/19/entrez
KW - Clostridium difficile
KW - MLVA
KW - PCR ribotype 001
KW - Thr82Ile
KW - antimicrobial drug resistance
KW - capillary electrophoresis ribotyping
SP - 609
EP - 615
JF - Microbial drug resistance (Larchmont, N.Y.)
JO - Microb Drug Resist
VL - 23
IS - 5
N2 - AIM: To perform a retrospective analysis of the high occurrence of Clostridium difficile infection in the surgical department of a Czech tertiary care hospital and to identify weaknesses in C. difficile infection (CDI) prevention and control policies. METHODS: Clinical and epidemiological data on eleven CDI cases were collected. C. difficile isolates were characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat analysis (MLVA), gyrA gene fragment sequencing, and erm(B) fragment PCR amplification. Antibiotic susceptibility to metronidazole, vancomycin, ciprofloxacin, moxifloxacin, and clindamycin was tested. FINDINGS: Eleven CDI cases were caused by C. difficile PCR ribotype 001 strains. These strains revealed two different MLVA profiles with 11 tandem repeat differences. All isolates were susceptible to metronidazole and vancomycin and resistant to ciprofloxacin (MIC ≥32 mg/L), moxifloxacin (MIC ≥32 mg/L), and clindamycin (MIC ≥256 mg/L). All isolates revealed amino acid substitution Thr82Ile, in the GyrA and were erm(B) negative. CONCLUSION: Two fluoroquinolone and clindamycin-resistant C. difficile PCR ribotype 001 strain clusters occurred at one of the surgical departments of a tertiary care hospital. Ineffective decontamination with suboptimal concentration and time of exposure of sporicidal disinfectants may have resulted in C. difficile transmission.
SN - 1931-8448
UR - https://www.unboundmedicine.com/medline/citation/27860531/Two_Clusters_of_Fluoroquinolone_and_Clindamycin_Resistant_Clostridium_difficile_PCR_Ribotype_001_Strain_Recognized_by_Capillary_Electrophoresis_Ribotyping_and_Multilocus_Variable_Tandem_Repeat_Analysis_
L2 - https://www.liebertpub.com/doi/10.1089/mdr.2016.0159?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed
DB - PRIME
DP - Unbound Medicine
ER -