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A role for the thymic epithelium in the selection of pre-T cells from murine bone marrow.
J Immunol. 1989 Aug 15; 143(4):1077-86.JI

Abstract

A rat thymic epithelial cell line IT45-R1 has been previously described as secreting soluble molecules that in vitro chemoattract rat hemopoietic precursor cells. The development of such an in vitro migration assay was based on the ability of cells to migrate across polycarbonate filters in Boyden chambers. In the present paper, by using the same strategy, we studied murine bone marrow cells capable of migrating in vitro toward IT45-R1 conditioned medium. The responding cells were shown to represent a minor bone marrow subpopulation characterized by a low capacity to incorporate tritiated thymidine in vitro (less than 10% of control). Moreover, this cell subset was considerably impoverished with respect to granulocyte-macrophage CFU (less than 7% of control) and pluripotent hemopoietic stem cells (less than 12% of control). Potential generation of T cells of donor-type in the lymphoid organs of irradiated recipients was measured by using C57BL/Ka Thy-1.1 and Thy-1.2 congenic mice. Thy-1.1 irradiated mice were injected intrathymically or intravenously with the selectively migrated cell subset of Thy-1.2 donor-type bone marrow cells. The use of an i.v. transfer route allowed us to show that these cells possess thymus-homing and colonization abilities. In a time-course study after intrathymic cell transfer, these migrated cells were able to generate Thy-1.2+ donor-type thymocytes represented by all cortical and medullary cell subsets in a single wave of repopulation from day 20 to day 30 after transfer, with a peak around days 23 to 25. The degree of repopulation closely resembled that seen with unfractionated bone marrow cells in terms of absolute numbers of donor cells per thymus (82% of control, 22 x 10(6) Thy-1.2+ cells) as well as in percent donor cells per thymus (105% of control). Thy-1.2+ cells were also detected in the lymph nodes and the spleens of reconstituted recipient mice. Taken together, these results support the idea that the supernatant of the established thymic epithelium IT45-R1 induces the migration of a murine bone marrow subset that contains hemopoietic stem cells already committed to the lymphoid lineage (i.e., pre-T cells).

Authors+Show Affiliations

Laboratoire de Physiopathologie du Développement, CNRS URA 230, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2787355

Citation

Bauvois, B, et al. "A Role for the Thymic Epithelium in the Selection of pre-T Cells From Murine Bone Marrow." Journal of Immunology (Baltimore, Md. : 1950), vol. 143, no. 4, 1989, pp. 1077-86.
Bauvois B, Ezine S, Imhof B, et al. A role for the thymic epithelium in the selection of pre-T cells from murine bone marrow. J Immunol. 1989;143(4):1077-86.
Bauvois, B., Ezine, S., Imhof, B., Denoyelle, M., & Thiery, J. P. (1989). A role for the thymic epithelium in the selection of pre-T cells from murine bone marrow. Journal of Immunology (Baltimore, Md. : 1950), 143(4), 1077-86.
Bauvois B, et al. A Role for the Thymic Epithelium in the Selection of pre-T Cells From Murine Bone Marrow. J Immunol. 1989 Aug 15;143(4):1077-86. PubMed PMID: 2787355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A role for the thymic epithelium in the selection of pre-T cells from murine bone marrow. AU - Bauvois,B, AU - Ezine,S, AU - Imhof,B, AU - Denoyelle,M, AU - Thiery,J P, PY - 1989/8/15/pubmed PY - 1989/8/15/medline PY - 1989/8/15/entrez SP - 1077 EP - 86 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 143 IS - 4 N2 - A rat thymic epithelial cell line IT45-R1 has been previously described as secreting soluble molecules that in vitro chemoattract rat hemopoietic precursor cells. The development of such an in vitro migration assay was based on the ability of cells to migrate across polycarbonate filters in Boyden chambers. In the present paper, by using the same strategy, we studied murine bone marrow cells capable of migrating in vitro toward IT45-R1 conditioned medium. The responding cells were shown to represent a minor bone marrow subpopulation characterized by a low capacity to incorporate tritiated thymidine in vitro (less than 10% of control). Moreover, this cell subset was considerably impoverished with respect to granulocyte-macrophage CFU (less than 7% of control) and pluripotent hemopoietic stem cells (less than 12% of control). Potential generation of T cells of donor-type in the lymphoid organs of irradiated recipients was measured by using C57BL/Ka Thy-1.1 and Thy-1.2 congenic mice. Thy-1.1 irradiated mice were injected intrathymically or intravenously with the selectively migrated cell subset of Thy-1.2 donor-type bone marrow cells. The use of an i.v. transfer route allowed us to show that these cells possess thymus-homing and colonization abilities. In a time-course study after intrathymic cell transfer, these migrated cells were able to generate Thy-1.2+ donor-type thymocytes represented by all cortical and medullary cell subsets in a single wave of repopulation from day 20 to day 30 after transfer, with a peak around days 23 to 25. The degree of repopulation closely resembled that seen with unfractionated bone marrow cells in terms of absolute numbers of donor cells per thymus (82% of control, 22 x 10(6) Thy-1.2+ cells) as well as in percent donor cells per thymus (105% of control). Thy-1.2+ cells were also detected in the lymph nodes and the spleens of reconstituted recipient mice. Taken together, these results support the idea that the supernatant of the established thymic epithelium IT45-R1 induces the migration of a murine bone marrow subset that contains hemopoietic stem cells already committed to the lymphoid lineage (i.e., pre-T cells). SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2787355/A_role_for_the_thymic_epithelium_in_the_selection_of_pre_T_cells_from_murine_bone_marrow_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2787355 DB - PRIME DP - Unbound Medicine ER -