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Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa).
Plant Physiol Biochem. 2017 Feb; 111:1-9.PP

Abstract

Leaf-color mutants have been extensively studied in rice, and many corresponding genes have been identified up to now. However, leaf-color mutation mechanisms are diverse and still need further research through identification of novel genes. In the present paper, we isolated a leaf-color mutant, ys83, in rice (Oryza sativa). The mutant displayed a yellow-green leaf phenotype at seedling stage, and then slowly turned into light-green leaf from late tillering stage. In its yellow leaves, photosynthetic pigment contents significantly decreased and the chloroplast development was retarded. The mutant phenotype was controlled by a recessive mutation in a nuclear gene on the short arm of rice chromosome 2. Map-based cloning and sequencing analysis suggested that the candidate gene was YS83 (LOC_Os02g05890) encoding a protein containing 165 amino acid residues. Gene YS83 was expressed in a wide range of tissues, and its encoded protein was targeted to the chloroplast. In the mutant, a T-to-A substitution occurred in coding sequence of gene YS83, which caused a premature translation of its encoded product. By introduction of the wild-type gene, the ys83 mutant recovered to normal green-leaf phenotype. Taken together, we successfully identified a novel yellow-green leaf gene YS83. In addition, number of productive panicles per plant and number of spikelets per panicle only reduced by 6.7% and 7.6%, respectively, meanwhile its seed setting rate and 1000-grain weight (seed size) were not significantly affected in the mutant, so leaf-color mutant gene ys83 could be used as a trait marker gene in commercial hybrid rice production.

Authors+Show Affiliations

Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; School of Automobile & Transportation, Xihua University, Chengdu 610039, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: prwang@sicau.edu.cn.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: xjdeng2006@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27875742

Citation

Ma, Xiaozhi, et al. "Map-based Cloning and Characterization of the Novel Yellow-green Leaf Gene Ys83 in Rice (Oryza Sativa)." Plant Physiology and Biochemistry : PPB, vol. 111, 2017, pp. 1-9.
Ma X, Sun X, Li C, et al. Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa). Plant Physiol Biochem. 2017;111:1-9.
Ma, X., Sun, X., Li, C., Huan, R., Sun, C., Wang, Y., Xiao, F., Wang, Q., Chen, P., Ma, F., Zhang, K., Wang, P., & Deng, X. (2017). Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa). Plant Physiology and Biochemistry : PPB, 111, 1-9. https://doi.org/10.1016/j.plaphy.2016.11.007
Ma X, et al. Map-based Cloning and Characterization of the Novel Yellow-green Leaf Gene Ys83 in Rice (Oryza Sativa). Plant Physiol Biochem. 2017;111:1-9. PubMed PMID: 27875742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa). AU - Ma,Xiaozhi, AU - Sun,Xiaoqiu, AU - Li,Chunmei, AU - Huan,Rui, AU - Sun,Changhui, AU - Wang,Yang, AU - Xiao,Fuliang, AU - Wang,Qian, AU - Chen,Purui, AU - Ma,Furong, AU - Zhang,Kuan, AU - Wang,Pingrong, AU - Deng,Xiaojian, Y1 - 2016/11/09/ PY - 2016/09/04/received PY - 2016/10/25/revised PY - 2016/11/06/accepted PY - 2016/11/23/pubmed PY - 2017/5/2/medline PY - 2016/11/23/entrez KW - Chlorophyll deficient KW - Gene cloning KW - Rice KW - Yellow-green leaf mutant SP - 1 EP - 9 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 111 N2 - Leaf-color mutants have been extensively studied in rice, and many corresponding genes have been identified up to now. However, leaf-color mutation mechanisms are diverse and still need further research through identification of novel genes. In the present paper, we isolated a leaf-color mutant, ys83, in rice (Oryza sativa). The mutant displayed a yellow-green leaf phenotype at seedling stage, and then slowly turned into light-green leaf from late tillering stage. In its yellow leaves, photosynthetic pigment contents significantly decreased and the chloroplast development was retarded. The mutant phenotype was controlled by a recessive mutation in a nuclear gene on the short arm of rice chromosome 2. Map-based cloning and sequencing analysis suggested that the candidate gene was YS83 (LOC_Os02g05890) encoding a protein containing 165 amino acid residues. Gene YS83 was expressed in a wide range of tissues, and its encoded protein was targeted to the chloroplast. In the mutant, a T-to-A substitution occurred in coding sequence of gene YS83, which caused a premature translation of its encoded product. By introduction of the wild-type gene, the ys83 mutant recovered to normal green-leaf phenotype. Taken together, we successfully identified a novel yellow-green leaf gene YS83. In addition, number of productive panicles per plant and number of spikelets per panicle only reduced by 6.7% and 7.6%, respectively, meanwhile its seed setting rate and 1000-grain weight (seed size) were not significantly affected in the mutant, so leaf-color mutant gene ys83 could be used as a trait marker gene in commercial hybrid rice production. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27875742/Map_based_cloning_and_characterization_of_the_novel_yellow_green_leaf_gene_ys83_in_rice__Oryza_sativa__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30427-2 DB - PRIME DP - Unbound Medicine ER -