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A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet.
Front Physiol 2016; 7:533FP

Abstract

Purpose:

Ketogenic diets (KD) can facilitate weight loss, but their effects on skeletal muscle remain equivocal. In this experiment we investigated the effects of two diets on skeletal muscle mitochondrial coupling, mitochondrial complex activity, markers of oxidative stress, and gene expression in sedentary and resistance exercised rats.

Methods:

Male Sprague-Dawley rats (9-10 weeks of age, 300-325 g) were fed isocaloric amounts of either a KD (17 g/day, 5.2 kcal/g, 20.2% protein, 10.3% CHO, 69.5% fat, n = 16) or a Western diet (WD) (20 g/day, 4.5 kcal/g, 15.2% protein, 42.7% CHO, 42.0% fat, n = 16) for 6 weeks. During these 6 weeks animals were either sedentary (SED, n = 8 per diet group) or voluntarily exercised using resistance-loaded running wheels (EXE, n = 8 per diet group). Gastrocnemius was excised and used for mitochondrial isolation and biochemical analyses.

Results:

In the presence of a complex II substrate, the respiratory control ratio (RCR) of isolated gastrocnemius mitochondria was higher (p < 0.05) in animals fed the KD compared to animals fed the WD. Complex I and IV enzyme activity was higher (p < 0.05) in EXE animals regardless of diet. SOD2 protein levels and GLUT4 and PGC1α mRNA expression were higher (p < 0.05) in EXE animals regardless of diet.

Conclusion:

Our data indicate that skeletal muscle mitochondrial coupling of complex II substrates is more efficient in chronically resistance trained rodents fed a KD. These findings may provide merit for further investigation, perhaps on humans.

Authors+Show Affiliations

School of Kinesiology, Auburn University Auburn, AL, USA.School of Kinesiology, Auburn University Auburn, AL, USA.School of Kinesiology, Auburn University Auburn, AL, USA.School of Kinesiology, Auburn University Auburn, AL, USA.School of Kinesiology, Auburn University Auburn, AL, USA.Department of Health Sciences and Human Performance, University of Tampa Tampa, FL, USA.School of Kinesiology, Auburn University Auburn, AL, USA.Department of Health Sciences and Human Performance, University of Tampa Tampa, FL, USA.School of Kinesiology, Auburn University Auburn, AL, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27877138

Citation

Hyatt, Hayden W., et al. "A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet." Frontiers in Physiology, vol. 7, 2016, p. 533.
Hyatt HW, Kephart WC, Holland AM, et al. A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet. Front Physiol. 2016;7:533.
Hyatt, H. W., Kephart, W. C., Holland, A. M., Mumford, P., Mobley, C. B., Lowery, R. P., ... Kavazis, A. N. (2016). A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet. Frontiers in Physiology, 7, p. 533.
Hyatt HW, et al. A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet. Front Physiol. 2016;7:533. PubMed PMID: 27877138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet. AU - Hyatt,Hayden W, AU - Kephart,Wesley C, AU - Holland,A Maleah, AU - Mumford,Petey, AU - Mobley,C Brooks, AU - Lowery,Ryan P, AU - Roberts,Michael D, AU - Wilson,Jacob M, AU - Kavazis,Andreas N, Y1 - 2016/11/08/ PY - 2016/09/09/received PY - 2016/10/26/accepted PY - 2016/11/24/entrez PY - 2016/11/24/pubmed PY - 2016/11/24/medline KW - antioxidants KW - exercise KW - ketogenic diets KW - mitochondria KW - oxidative stress SP - 533 EP - 533 JF - Frontiers in physiology JO - Front Physiol VL - 7 N2 - Purpose: Ketogenic diets (KD) can facilitate weight loss, but their effects on skeletal muscle remain equivocal. In this experiment we investigated the effects of two diets on skeletal muscle mitochondrial coupling, mitochondrial complex activity, markers of oxidative stress, and gene expression in sedentary and resistance exercised rats. Methods: Male Sprague-Dawley rats (9-10 weeks of age, 300-325 g) were fed isocaloric amounts of either a KD (17 g/day, 5.2 kcal/g, 20.2% protein, 10.3% CHO, 69.5% fat, n = 16) or a Western diet (WD) (20 g/day, 4.5 kcal/g, 15.2% protein, 42.7% CHO, 42.0% fat, n = 16) for 6 weeks. During these 6 weeks animals were either sedentary (SED, n = 8 per diet group) or voluntarily exercised using resistance-loaded running wheels (EXE, n = 8 per diet group). Gastrocnemius was excised and used for mitochondrial isolation and biochemical analyses. Results: In the presence of a complex II substrate, the respiratory control ratio (RCR) of isolated gastrocnemius mitochondria was higher (p < 0.05) in animals fed the KD compared to animals fed the WD. Complex I and IV enzyme activity was higher (p < 0.05) in EXE animals regardless of diet. SOD2 protein levels and GLUT4 and PGC1α mRNA expression were higher (p < 0.05) in EXE animals regardless of diet. Conclusion: Our data indicate that skeletal muscle mitochondrial coupling of complex II substrates is more efficient in chronically resistance trained rodents fed a KD. These findings may provide merit for further investigation, perhaps on humans. SN - 1664-042X UR - https://www.unboundmedicine.com/medline/citation/27877138/A_Ketogenic_Diet_in_Rodents_Elicits_Improved_Mitochondrial_Adaptations_in_Response_to_Resistance_Exercise_Training_Compared_to_an_Isocaloric_Western_Diet_ L2 - https://dx.doi.org/10.3389/fphys.2016.00533 DB - PRIME DP - Unbound Medicine ER -