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Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions.
Front Microbiol 2016; 7:1769FM

Abstract

Native lactic acid bacteria (LAB) are capable of growing during winemaking, thereby strongly affecting wine quality. The species of LAB present in musts, wines during malolactic fermentation (MLF), and barrels/filters were investigated in wineries from the emerging wine region of Queretaro, México using multiplex PCR and culture. The resistance to wine-like conditions (WLC): ethanol (10, 12, and 13%), SO2 (30 mg⋅l-1), and low pH (3.5) of native LAB strains was also studied. Five species were detected within 61 samples obtained: Oenococcus oeni, Lactobacillus plantarum, Pediococcus parvulus, Lactobacillus hilgardi, and Lactobacillus brevis. Four species (excepting L. brevis) were found in must; O. oeni and P. parvulus were ubiquitous in wine and L. plantarum and L. brevis were mainly present at the initial stage of MLF, while L. hilgardii was mostly detected at the advanced stage. Furthermore, some species detected in barrel/filter, prove them to be hazardous reservoirs. From 822 LAB isolates, only 119 resisted WLC with 10% ethanol; the number of strains able to grow in WLC with 13% ethanol decreased approximately by 50%, O. oeni being the most versatile species with 65% of resistant isolates, while Lactobacillus spp. and P. parvulus were the most strongly affected, especially those recovered from barrel/filter, with less than 10% of resistant isolates. This study evidences the presence of local strains able to be used as starter cultures, and also enabled the assessment of the risks derived from the presence of spoilage LAB strains resistant to WLC.

Authors+Show Affiliations

Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.Departamento de Investigación y Posgrado en Alimentos, Facultad de Química, Universidad Autónoma de Querétaro Santiago de Querétaro, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27877164

Citation

Miranda-Castilleja, Dalia E., et al. "Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions." Frontiers in Microbiology, vol. 7, 2016, p. 1769.
Miranda-Castilleja DE, Martínez-Peniche RÁ, Aldrete-Tapia JA, et al. Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions. Front Microbiol. 2016;7:1769.
Miranda-Castilleja, D. E., Martínez-Peniche, R. Á., Aldrete-Tapia, J. A., Soto-Muñoz, L., Iturriaga, M. H., Pacheco-Aguilar, J. R., & Arvizu-Medrano, S. M. (2016). Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions. Frontiers in Microbiology, 7, p. 1769.
Miranda-Castilleja DE, et al. Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions. Front Microbiol. 2016;7:1769. PubMed PMID: 27877164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions. AU - Miranda-Castilleja,Dalia E, AU - Martínez-Peniche,Ramón Álvar, AU - Aldrete-Tapia,J A, AU - Soto-Muñoz,Lourdes, AU - Iturriaga,Montserrat H, AU - Pacheco-Aguilar,J R, AU - Arvizu-Medrano,Sofía M, Y1 - 2016/11/08/ PY - 2016/08/17/received PY - 2016/09/21/accepted PY - 2016/11/24/entrez PY - 2016/11/24/pubmed PY - 2016/11/24/medline KW - Oenococcus oeni KW - malolactic fermentation KW - multiplex PCR KW - starter cultures KW - wine spoilage SP - 1769 EP - 1769 JF - Frontiers in microbiology JO - Front Microbiol VL - 7 N2 - Native lactic acid bacteria (LAB) are capable of growing during winemaking, thereby strongly affecting wine quality. The species of LAB present in musts, wines during malolactic fermentation (MLF), and barrels/filters were investigated in wineries from the emerging wine region of Queretaro, México using multiplex PCR and culture. The resistance to wine-like conditions (WLC): ethanol (10, 12, and 13%), SO2 (30 mg⋅l-1), and low pH (3.5) of native LAB strains was also studied. Five species were detected within 61 samples obtained: Oenococcus oeni, Lactobacillus plantarum, Pediococcus parvulus, Lactobacillus hilgardi, and Lactobacillus brevis. Four species (excepting L. brevis) were found in must; O. oeni and P. parvulus were ubiquitous in wine and L. plantarum and L. brevis were mainly present at the initial stage of MLF, while L. hilgardii was mostly detected at the advanced stage. Furthermore, some species detected in barrel/filter, prove them to be hazardous reservoirs. From 822 LAB isolates, only 119 resisted WLC with 10% ethanol; the number of strains able to grow in WLC with 13% ethanol decreased approximately by 50%, O. oeni being the most versatile species with 65% of resistant isolates, while Lactobacillus spp. and P. parvulus were the most strongly affected, especially those recovered from barrel/filter, with less than 10% of resistant isolates. This study evidences the presence of local strains able to be used as starter cultures, and also enabled the assessment of the risks derived from the presence of spoilage LAB strains resistant to WLC. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/27877164/Distribution_of_Native_Lactic_Acid_Bacteria_in_Wineries_of_Queretaro_Mexico_and_Their_Resistance_to_Wine_Like_Conditions_ L2 - https://doi.org/10.3389/fmicb.2016.01769 DB - PRIME DP - Unbound Medicine ER -