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Altered growth factor sensitivity in EL2 rat fibroblasts: influence of this biological characteristic on cell growth.
Eur J Cell Biol. 1989 Jun; 49(1):196-201.EJ

Abstract

Extensive evidence indicate that platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) play a key role in the stimulation of the 3T3 fibroblast replication: in this connection, PDGF and EGF act as a competence and a progression factor, respectively. We have previously demonstrated that EGF alone leads density-arrested EL2 rat fibroblasts to synthesize DNA and proliferate in serum-free cultures. Here, we have analyzed the role of EGF in the control of EL2 cell proliferation. Our data show a dose-related effect of EGF on DNA synthesis and cell growth, with maximal stimulation for both parameters at 20 ng/ml. On the other hand, autocrine production of PDGF or PDGF-like substances by EL2 cells is seemingly excluded by experiments with anti-PDGF serum or medium conditioned by EL2 fibroblasts. EGF binding studies show that EL2 cells possess high affinity EGF receptors, at a density level 3 to 4-fold higher than other fibroblastic lines. In addition, EL2 cells show a normal down-regulation of EGF receptors, following exposure to EGF, but PDGF, fibroblast growth factor (FGF), transforming growth factor beta (TGF beta) and bombesin have not decreased the affinity of EGF receptor for its ligand. Moreover, in EL2 cells, the EGF is able to induce the synthesis of putative intracellular regulatory proteins that govern the PDGF-induced competence in 3T3 cells. Our data indicate that EGF in EL2 cells may act as both a competence and a progression factor, via induction of the mechanisms, regulated in other cell lines by cooperation between different growth factors.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Experimental Medicine and Biochemical Sciences, 2nd University of Rome, Tor Vergata, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2788084

Citation

Di Francesco, P, et al. "Altered Growth Factor Sensitivity in EL2 Rat Fibroblasts: Influence of This Biological Characteristic On Cell Growth." European Journal of Cell Biology, vol. 49, no. 1, 1989, pp. 196-201.
Di Francesco P, Testa EP, Testa U, et al. Altered growth factor sensitivity in EL2 rat fibroblasts: influence of this biological characteristic on cell growth. Eur J Cell Biol. 1989;49(1):196-201.
Di Francesco, P., Testa, E. P., Testa, U., & Liboi, E. (1989). Altered growth factor sensitivity in EL2 rat fibroblasts: influence of this biological characteristic on cell growth. European Journal of Cell Biology, 49(1), 196-201.
Di Francesco P, et al. Altered Growth Factor Sensitivity in EL2 Rat Fibroblasts: Influence of This Biological Characteristic On Cell Growth. Eur J Cell Biol. 1989;49(1):196-201. PubMed PMID: 2788084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered growth factor sensitivity in EL2 rat fibroblasts: influence of this biological characteristic on cell growth. AU - Di Francesco,P, AU - Testa,E P, AU - Testa,U, AU - Liboi,E, PY - 1989/6/1/pubmed PY - 1989/6/1/medline PY - 1989/6/1/entrez SP - 196 EP - 201 JF - European journal of cell biology JO - Eur J Cell Biol VL - 49 IS - 1 N2 - Extensive evidence indicate that platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) play a key role in the stimulation of the 3T3 fibroblast replication: in this connection, PDGF and EGF act as a competence and a progression factor, respectively. We have previously demonstrated that EGF alone leads density-arrested EL2 rat fibroblasts to synthesize DNA and proliferate in serum-free cultures. Here, we have analyzed the role of EGF in the control of EL2 cell proliferation. Our data show a dose-related effect of EGF on DNA synthesis and cell growth, with maximal stimulation for both parameters at 20 ng/ml. On the other hand, autocrine production of PDGF or PDGF-like substances by EL2 cells is seemingly excluded by experiments with anti-PDGF serum or medium conditioned by EL2 fibroblasts. EGF binding studies show that EL2 cells possess high affinity EGF receptors, at a density level 3 to 4-fold higher than other fibroblastic lines. In addition, EL2 cells show a normal down-regulation of EGF receptors, following exposure to EGF, but PDGF, fibroblast growth factor (FGF), transforming growth factor beta (TGF beta) and bombesin have not decreased the affinity of EGF receptor for its ligand. Moreover, in EL2 cells, the EGF is able to induce the synthesis of putative intracellular regulatory proteins that govern the PDGF-induced competence in 3T3 cells. Our data indicate that EGF in EL2 cells may act as both a competence and a progression factor, via induction of the mechanisms, regulated in other cell lines by cooperation between different growth factors.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0171-9335 UR - https://www.unboundmedicine.com/medline/citation/2788084/Altered_growth_factor_sensitivity_in_EL2_rat_fibroblasts:_influence_of_this_biological_characteristic_on_cell_growth_ DB - PRIME DP - Unbound Medicine ER -