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Neonatal Repeated Exposure to Isoflurane not Sevoflurane in Mice Reversibly Impaired Spatial Cognition at Juvenile-Age.
Neurochem Res. 2017 Feb; 42(2):595-605.NR

Abstract

Inhalation anesthetics facilitate surgical procedures in millions of children each year. However, animal studies demonstrate that exposure to the inhalation anesthetic isoflurane may cause neuronal cell death in developing brains. The long-term cytotoxic effects of sevoflurane, the most popular pediatric anesthetic, have not been compared with isoflurane. Thus, this study was designed to compare the effects of equipotent doses of these two anesthetics on neonatal long-term neurotoxicity. Postnatal 7-day-old (P7) C57/BL male mice were exposed to 1.5% isoflurane or 2.2% sevoflurane 2 h a day for 3 days. Non-anesthetized mice served as controls. The effects of anesthesia on learning and memory were assessed using the Morris Water Maze (MWM) at Postnatal days 30 (P30) and P60 respectively. The hippocampal content of N-methyl-D-aspartate receptor subunits (NMDA), brain-derived neurotrophic factor (BDNF), and synaptophysin (Syn) were determined by Western Blot. Neuron structure and apoptosis were assessed via Nissl and TUNEL staining, respectively. The isoflurane group exhibited cognitive impairment at P30. Repeated inhalation of isoflurane or sevoflurane caused different degrees of apoptosis and damaged hippocampal neurons in neonatal mice, particularly isoflurane. In neonatal mice, repeated exposure to isoflurane, but not sevoflurane, caused spatial cognitive impairments in juvenile mice. Our findings suggest that isoflurane induces significantly greater neurodegeneration than an equipotent minimum alveolar concentration of sevoflurane.

Authors+Show Affiliations

Department of Anesthesiology, Tongji Hospital, Tongji University, Shanghai, 200065, China. jianhuiliu_1246@163.com.Department of Anesthesiology, Tongji Hospital, Tongji University, Shanghai, 200065, China.Department of Anesthesiology, Tongji Hospital, Tongji University, Shanghai, 200065, China.Department of Anesthesiology, Tongji Hospital, Tongji University, Shanghai, 200065, China.Department of Image and Radiology, Renji Hospital, Jiaotong University, Shanghai, 200127, China.Department of Image and Radiology, Tongji Hospital, Tongji University, Shanghai, 200065, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27882447

Citation

Liu, Jianhui, et al. "Neonatal Repeated Exposure to Isoflurane Not Sevoflurane in Mice Reversibly Impaired Spatial Cognition at Juvenile-Age." Neurochemical Research, vol. 42, no. 2, 2017, pp. 595-605.
Liu J, Zhao Y, Yang J, et al. Neonatal Repeated Exposure to Isoflurane not Sevoflurane in Mice Reversibly Impaired Spatial Cognition at Juvenile-Age. Neurochem Res. 2017;42(2):595-605.
Liu, J., Zhao, Y., Yang, J., Zhang, X., Zhang, W., & Wang, P. (2017). Neonatal Repeated Exposure to Isoflurane not Sevoflurane in Mice Reversibly Impaired Spatial Cognition at Juvenile-Age. Neurochemical Research, 42(2), 595-605. https://doi.org/10.1007/s11064-016-2114-7
Liu J, et al. Neonatal Repeated Exposure to Isoflurane Not Sevoflurane in Mice Reversibly Impaired Spatial Cognition at Juvenile-Age. Neurochem Res. 2017;42(2):595-605. PubMed PMID: 27882447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neonatal Repeated Exposure to Isoflurane not Sevoflurane in Mice Reversibly Impaired Spatial Cognition at Juvenile-Age. AU - Liu,Jianhui, AU - Zhao,Yanhong, AU - Yang,Junjun, AU - Zhang,Xiaoqing, AU - Zhang,Wei, AU - Wang,Peijun, Y1 - 2016/11/24/ PY - 2016/11/12/received PY - 2016/11/16/accepted PY - 2016/11/25/pubmed PY - 2017/3/8/medline PY - 2016/11/25/entrez KW - Cognitive impairment KW - Hippocampus KW - Isoflurane KW - Sevoflurane SP - 595 EP - 605 JF - Neurochemical research JO - Neurochem. Res. VL - 42 IS - 2 N2 - Inhalation anesthetics facilitate surgical procedures in millions of children each year. However, animal studies demonstrate that exposure to the inhalation anesthetic isoflurane may cause neuronal cell death in developing brains. The long-term cytotoxic effects of sevoflurane, the most popular pediatric anesthetic, have not been compared with isoflurane. Thus, this study was designed to compare the effects of equipotent doses of these two anesthetics on neonatal long-term neurotoxicity. Postnatal 7-day-old (P7) C57/BL male mice were exposed to 1.5% isoflurane or 2.2% sevoflurane 2 h a day for 3 days. Non-anesthetized mice served as controls. The effects of anesthesia on learning and memory were assessed using the Morris Water Maze (MWM) at Postnatal days 30 (P30) and P60 respectively. The hippocampal content of N-methyl-D-aspartate receptor subunits (NMDA), brain-derived neurotrophic factor (BDNF), and synaptophysin (Syn) were determined by Western Blot. Neuron structure and apoptosis were assessed via Nissl and TUNEL staining, respectively. The isoflurane group exhibited cognitive impairment at P30. Repeated inhalation of isoflurane or sevoflurane caused different degrees of apoptosis and damaged hippocampal neurons in neonatal mice, particularly isoflurane. In neonatal mice, repeated exposure to isoflurane, but not sevoflurane, caused spatial cognitive impairments in juvenile mice. Our findings suggest that isoflurane induces significantly greater neurodegeneration than an equipotent minimum alveolar concentration of sevoflurane. SN - 1573-6903 UR - https://www.unboundmedicine.com/medline/citation/27882447/Neonatal_Repeated_Exposure_to_Isoflurane_not_Sevoflurane_in_Mice_Reversibly_Impaired_Spatial_Cognition_at_Juvenile_Age_ L2 - https://doi.org/10.1007/s11064-016-2114-7 DB - PRIME DP - Unbound Medicine ER -