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Long Non-Coding RNA Uc.187 Is Upregulated in Preeclampsia and Modulates Proliferation, Apoptosis, and Invasion of HTR-8/SVneo Trophoblast Cells.
J Cell Biochem 2017; 118(6):1462-1470JC

Abstract

Among the preeclampsia-related long non-cording RNAs (lncRNAs) screened with a gene chip in our preliminary study, uc.187 attracted our attention because of its high conservation across different species and significant positive correlation with preeclampsia (PE). The literature and bioinformatics analysis suggested that lncRNA uc.187 might be associated with cell growth, invasion, and apoptosis. The expression of uc.187 in severe preeclamptic placentas (n = 31) and normal placentas (n = 18) was evaluated by real-time reverse transcription polymerase chain reaction (qRT-PCR). We constructed a silencing lentivirus vector (uc.187 siRNA) to explore the biological function of uc.187 in the development and progression of HTR-8/SVneo trophoblast cells in vitro. Furthermore, we utilized CCK8 analysis, a transwell invasion assay, and flow cytometry to determine the role of uc.187 in the proliferation, invasion, and apoptosis of HTR-8/SVneo trophoblast cells. The proteins related to proliferation (PCNA, Ki67), invasion (MMP-2/-9 and TIMP-1), and apoptosis (caspase-3, Bcl-2) were evaluated with a Western blot assay. The results showed that there was an obvious upregulation of uc.187 expression in preeclamptic placental tissues. In addition, uc.187 silencing enhanced cell proliferation and invasion and reduced the cellular apoptotic response. Taken together, our findings suggest for the first time that abnormal expression of lncRNA uc.187 may lead to the aberrant biological behavior of HTR-8/SVneo cells. Therefore, we propose uc.187 as a novel lncRNA molecule that might contribute to the development of PE and might represent a potential diagnostic and therapeutic target for this disease. J. Cell. Biochem. 118: 1462-1470, 2017. © 2016 Wiley Periodicals, Inc.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Maternal and Child Health Care Hospital of Nantong, Nantong, Jiangsu 226018, China.Department of Plastic & Cosmetic Surgery, State key Laboratory of Reproductive Medicine, Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.Department of Plastic & Cosmetic Surgery, State key Laboratory of Reproductive Medicine, Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.Department of Obstetrics and Gynecology, Maternal and Child Health Care Hospital of Nantong, Nantong, Jiangsu 226018, China.Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27883216

Citation

Cao, Chunyu, et al. "Long Non-Coding RNA Uc.187 Is Upregulated in Preeclampsia and Modulates Proliferation, Apoptosis, and Invasion of HTR-8/SVneo Trophoblast Cells." Journal of Cellular Biochemistry, vol. 118, no. 6, 2017, pp. 1462-1470.
Cao C, Li J, Li J, et al. Long Non-Coding RNA Uc.187 Is Upregulated in Preeclampsia and Modulates Proliferation, Apoptosis, and Invasion of HTR-8/SVneo Trophoblast Cells. J Cell Biochem. 2017;118(6):1462-1470.
Cao, C., Li, J., Li, J., Liu, L., Cheng, X., & Jia, R. (2017). Long Non-Coding RNA Uc.187 Is Upregulated in Preeclampsia and Modulates Proliferation, Apoptosis, and Invasion of HTR-8/SVneo Trophoblast Cells. Journal of Cellular Biochemistry, 118(6), pp. 1462-1470. doi:10.1002/jcb.25805.
Cao C, et al. Long Non-Coding RNA Uc.187 Is Upregulated in Preeclampsia and Modulates Proliferation, Apoptosis, and Invasion of HTR-8/SVneo Trophoblast Cells. J Cell Biochem. 2017;118(6):1462-1470. PubMed PMID: 27883216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long Non-Coding RNA Uc.187 Is Upregulated in Preeclampsia and Modulates Proliferation, Apoptosis, and Invasion of HTR-8/SVneo Trophoblast Cells. AU - Cao,Chunyu, AU - Li,Jingyun, AU - Li,Jun, AU - Liu,Lan, AU - Cheng,Xiaoyan, AU - Jia,Ruizhe, Y1 - 2017/01/10/ PY - 2016/09/21/received PY - 2016/11/22/accepted PY - 2016/11/25/pubmed PY - 2017/11/29/medline PY - 2016/11/25/entrez KW - APOPTOSIS KW - INVASION KW - LONG NON-CODING RNA KW - PROLIFERATION KW - TROPHOBLAST CELLS KW - lncRNA UC.187 SP - 1462 EP - 1470 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 118 IS - 6 N2 - Among the preeclampsia-related long non-cording RNAs (lncRNAs) screened with a gene chip in our preliminary study, uc.187 attracted our attention because of its high conservation across different species and significant positive correlation with preeclampsia (PE). The literature and bioinformatics analysis suggested that lncRNA uc.187 might be associated with cell growth, invasion, and apoptosis. The expression of uc.187 in severe preeclamptic placentas (n = 31) and normal placentas (n = 18) was evaluated by real-time reverse transcription polymerase chain reaction (qRT-PCR). We constructed a silencing lentivirus vector (uc.187 siRNA) to explore the biological function of uc.187 in the development and progression of HTR-8/SVneo trophoblast cells in vitro. Furthermore, we utilized CCK8 analysis, a transwell invasion assay, and flow cytometry to determine the role of uc.187 in the proliferation, invasion, and apoptosis of HTR-8/SVneo trophoblast cells. The proteins related to proliferation (PCNA, Ki67), invasion (MMP-2/-9 and TIMP-1), and apoptosis (caspase-3, Bcl-2) were evaluated with a Western blot assay. The results showed that there was an obvious upregulation of uc.187 expression in preeclamptic placental tissues. In addition, uc.187 silencing enhanced cell proliferation and invasion and reduced the cellular apoptotic response. Taken together, our findings suggest for the first time that abnormal expression of lncRNA uc.187 may lead to the aberrant biological behavior of HTR-8/SVneo cells. Therefore, we propose uc.187 as a novel lncRNA molecule that might contribute to the development of PE and might represent a potential diagnostic and therapeutic target for this disease. J. Cell. Biochem. 118: 1462-1470, 2017. © 2016 Wiley Periodicals, Inc. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/27883216/Long_Non_Coding_RNA_Uc_187_Is_Upregulated_in_Preeclampsia_and_Modulates_Proliferation_Apoptosis_and_Invasion_of_HTR_8/SVneo_Trophoblast_Cells_ L2 - https://doi.org/10.1002/jcb.25805 DB - PRIME DP - Unbound Medicine ER -