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Comparison of methenamine silver nitrate and Giemsa stain for detection of Pneumocystis carinii in bronchoalveolar lavage specimens from HIV infected patients.
APMIS. 1989 Aug; 97(8):745-7.A

Abstract

Seventy-seven bronchoalveolar lavages from human immunosuppressive virus infected patients with pulmonary symptoms were examined routinely for the presence of Pneumocystis carinii, using Giemsa stain to detect trophozoites and methenamine silver nitrate to detect cysts. Thirty-seven samples were found positive with both methods, but a further 10 were diagnosed with Giemsa indicating that the trophozoite stain is more sensitive. As Giemsa stain is simple, quick, cheap and familiar to most microbiological laboratories it should be used for screening of samples to be examined for Pneumocystis carinii. In cases where trophozoites are not detected by Giemsa a supplementary cyst stain may be performed in order to rule out the presumably few cases where cysts are present as the only evidence of Pneumocystis carinii infection.

Authors+Show Affiliations

Department of Clinical Microbiology, Hvidovre Hospital, Copenhagen, Denmark.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2788442

Citation

Holten-Andersen, W, and H J. Kolmos. "Comparison of Methenamine Silver Nitrate and Giemsa Stain for Detection of Pneumocystis Carinii in Bronchoalveolar Lavage Specimens From HIV Infected Patients." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 97, no. 8, 1989, pp. 745-7.
Holten-Andersen W, Kolmos HJ. Comparison of methenamine silver nitrate and Giemsa stain for detection of Pneumocystis carinii in bronchoalveolar lavage specimens from HIV infected patients. APMIS. 1989;97(8):745-7.
Holten-Andersen, W., & Kolmos, H. J. (1989). Comparison of methenamine silver nitrate and Giemsa stain for detection of Pneumocystis carinii in bronchoalveolar lavage specimens from HIV infected patients. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 97(8), 745-7.
Holten-Andersen W, Kolmos HJ. Comparison of Methenamine Silver Nitrate and Giemsa Stain for Detection of Pneumocystis Carinii in Bronchoalveolar Lavage Specimens From HIV Infected Patients. APMIS. 1989;97(8):745-7. PubMed PMID: 2788442.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of methenamine silver nitrate and Giemsa stain for detection of Pneumocystis carinii in bronchoalveolar lavage specimens from HIV infected patients. AU - Holten-Andersen,W, AU - Kolmos,H J, PY - 1989/8/1/pubmed PY - 1989/8/1/medline PY - 1989/8/1/entrez SP - 745 EP - 7 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 97 IS - 8 N2 - Seventy-seven bronchoalveolar lavages from human immunosuppressive virus infected patients with pulmonary symptoms were examined routinely for the presence of Pneumocystis carinii, using Giemsa stain to detect trophozoites and methenamine silver nitrate to detect cysts. Thirty-seven samples were found positive with both methods, but a further 10 were diagnosed with Giemsa indicating that the trophozoite stain is more sensitive. As Giemsa stain is simple, quick, cheap and familiar to most microbiological laboratories it should be used for screening of samples to be examined for Pneumocystis carinii. In cases where trophozoites are not detected by Giemsa a supplementary cyst stain may be performed in order to rule out the presumably few cases where cysts are present as the only evidence of Pneumocystis carinii infection. SN - 0903-4641 UR - https://www.unboundmedicine.com/medline/citation/2788442/Comparison_of_methenamine_silver_nitrate_and_Giemsa_stain_for_detection_of_Pneumocystis_carinii_in_bronchoalveolar_lavage_specimens_from_HIV_infected_patients_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0903-4641&date=1989&volume=97&spage=745 DB - PRIME DP - Unbound Medicine ER -