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Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach.
Yeast. 2017 04; 34(4):179-188.Y

Abstract

The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MSE system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. Copyright © 2016 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil. Escola de Engenharia, Pontifícia Universidade Católica de Goiás, 74605-010, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil. Programa de Pós-graduação em Patologia Molecular, Faculdade de Medicina, Universidade de Brasília, 70910-900, Brasília, Distrito Federal, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.Laboratório de Microscopia, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, 70910-900, Brasília, Distrito Federal, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27886402

Citation

Casaletti, L, et al. "Analysis of Paracoccidioides Lutzii Mitochondria: a Proteomic Approach." Yeast (Chichester, England), vol. 34, no. 4, 2017, pp. 179-188.
Casaletti L, Lima PS, Oliveira LN, et al. Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach. Yeast. 2017;34(4):179-188.
Casaletti, L., Lima, P. S., Oliveira, L. N., Borges, C. L., Báo, S. N., Bailão, A. M., & Soares, C. M. (2017). Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach. Yeast (Chichester, England), 34(4), 179-188. https://doi.org/10.1002/yea.3225
Casaletti L, et al. Analysis of Paracoccidioides Lutzii Mitochondria: a Proteomic Approach. Yeast. 2017;34(4):179-188. PubMed PMID: 27886402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach. AU - Casaletti,L, AU - Lima,P S, AU - Oliveira,L N, AU - Borges,C L, AU - Báo,S N, AU - Bailão,A M, AU - Soares,C M A, Y1 - 2017/01/26/ PY - 2016/06/27/received PY - 2016/11/16/revised PY - 2016/11/20/accepted PY - 2016/11/26/pubmed PY - 2017/12/12/medline PY - 2016/11/26/entrez KW - MSE proteomics KW - Paracoccidioides lutzii KW - metabolism and energy production KW - mitochondrial proteome SP - 179 EP - 188 JF - Yeast (Chichester, England) JO - Yeast VL - 34 IS - 4 N2 - The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MSE system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. Copyright © 2016 John Wiley & Sons, Ltd. SN - 1097-0061 UR - https://www.unboundmedicine.com/medline/citation/27886402/Analysis_of_Paracoccidioides_lutzii_mitochondria:_a_proteomic_approach_ L2 - https://doi.org/10.1002/yea.3225 DB - PRIME DP - Unbound Medicine ER -