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Genetic elements associated with antimicrobial resistance among avian pathogenic Escherichia coli.
Ann Clin Microbiol Antimicrob. 2016 Nov 25; 15(1):59.AC

Abstract

BACKGROUND

Avian-pathogenic Escherichia coli (APEC) are pathogenic strains of E. coli that are responsible for one of the most predominant bacterial disease affecting poultry worldwide called avian colibacillosis. This study describes the genetic determinants implicated in antimicrobial resistance among APEC isolated from different broiler farms in Egypt.

METHODS

A total of 116 APEC were investigated by serotyping, antimicrobial resistance patterns to 10 antimicrobials, and the genetic mechanisms underlying the antimicrobial-resistant phenotypes.

RESULTS

Antibiogram results showed that the highest resistance was observed for ampicillin, tetracycline, nalidixic acid, and chloramphenicol. The detected carriage rate of integron was 29.3% (34/116). Further characterization of gene cassettes revealed the presence gene cassettes encoding resistance to trimethoprim (dfrA1, dfrA5, dfrA7, dfrA12), streptomycin/spectinomycin (aadA1, aadA2, aadA5, aadA23), and streptothricin (sat2). To our knowledge, this the first description of the presence of aadA23 in APEC isolates. Analysis of other antimicrobial resistance types not associated with integrons revealed the predominance of resistance genes encoding resistance to tetracycline (tetA and tetB), ampicillin (bla TEM), chloramphenicol (cat1), kanamycin (aphA1), and sulphonamide (sul1 and sul2). Among ciprofloxacin-resistant isolates, the S83L mutation was the most frequently substitution observed in the quinolone resistance-determining region of gyrA (56.3%). The bla TEM and bla CTX-M-1 genes were the most prevalent among APEC isolates producing extended-spectrum beta-lactamase (ESβL).

CONCLUSIONS

These findings provided important clues about the role of integron-mediated resistance genes together with other independent resistance genes and chromosomal mutations in shaping the epidemiology of antimicrobial resistance in E. coli isolates from poultry farms in Egypt.

Authors+Show Affiliations

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.Department of Poultry diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. mm_elhadidy@mans.edu.eg. Foodborne Pathogens, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050, Brussels, Belgium. mm_elhadidy@mans.edu.eg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27887603

Citation

Awad, Amal, et al. "Genetic Elements Associated With Antimicrobial Resistance Among Avian Pathogenic Escherichia Coli." Annals of Clinical Microbiology and Antimicrobials, vol. 15, no. 1, 2016, p. 59.
Awad A, Arafat N, Elhadidy M. Genetic elements associated with antimicrobial resistance among avian pathogenic Escherichia coli. Ann Clin Microbiol Antimicrob. 2016;15(1):59.
Awad, A., Arafat, N., & Elhadidy, M. (2016). Genetic elements associated with antimicrobial resistance among avian pathogenic Escherichia coli. Annals of Clinical Microbiology and Antimicrobials, 15(1), 59.
Awad A, Arafat N, Elhadidy M. Genetic Elements Associated With Antimicrobial Resistance Among Avian Pathogenic Escherichia Coli. Ann Clin Microbiol Antimicrob. 2016 Nov 25;15(1):59. PubMed PMID: 27887603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic elements associated with antimicrobial resistance among avian pathogenic Escherichia coli. AU - Awad,Amal, AU - Arafat,Nagah, AU - Elhadidy,Mohamed, Y1 - 2016/11/25/ PY - 2016/09/02/received PY - 2016/11/17/accepted PY - 2016/11/27/entrez PY - 2016/11/27/pubmed PY - 2017/1/17/medline KW - Antimicrobial resistance KW - E. coli KW - Genes KW - Integron KW - Poultry SP - 59 EP - 59 JF - Annals of clinical microbiology and antimicrobials JO - Ann. Clin. Microbiol. Antimicrob. VL - 15 IS - 1 N2 - BACKGROUND: Avian-pathogenic Escherichia coli (APEC) are pathogenic strains of E. coli that are responsible for one of the most predominant bacterial disease affecting poultry worldwide called avian colibacillosis. This study describes the genetic determinants implicated in antimicrobial resistance among APEC isolated from different broiler farms in Egypt. METHODS: A total of 116 APEC were investigated by serotyping, antimicrobial resistance patterns to 10 antimicrobials, and the genetic mechanisms underlying the antimicrobial-resistant phenotypes. RESULTS: Antibiogram results showed that the highest resistance was observed for ampicillin, tetracycline, nalidixic acid, and chloramphenicol. The detected carriage rate of integron was 29.3% (34/116). Further characterization of gene cassettes revealed the presence gene cassettes encoding resistance to trimethoprim (dfrA1, dfrA5, dfrA7, dfrA12), streptomycin/spectinomycin (aadA1, aadA2, aadA5, aadA23), and streptothricin (sat2). To our knowledge, this the first description of the presence of aadA23 in APEC isolates. Analysis of other antimicrobial resistance types not associated with integrons revealed the predominance of resistance genes encoding resistance to tetracycline (tetA and tetB), ampicillin (bla TEM), chloramphenicol (cat1), kanamycin (aphA1), and sulphonamide (sul1 and sul2). Among ciprofloxacin-resistant isolates, the S83L mutation was the most frequently substitution observed in the quinolone resistance-determining region of gyrA (56.3%). The bla TEM and bla CTX-M-1 genes were the most prevalent among APEC isolates producing extended-spectrum beta-lactamase (ESβL). CONCLUSIONS: These findings provided important clues about the role of integron-mediated resistance genes together with other independent resistance genes and chromosomal mutations in shaping the epidemiology of antimicrobial resistance in E. coli isolates from poultry farms in Egypt. SN - 1476-0711 UR - https://www.unboundmedicine.com/medline/citation/27887603/Genetic_elements_associated_with_antimicrobial_resistance_among_avian_pathogenic_Escherichia_coli_ L2 - https://ann-clinmicrob.biomedcentral.com/articles/10.1186/s12941-016-0174-9 DB - PRIME DP - Unbound Medicine ER -