Tags

Type your tag names separated by a space and hit enter

Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin.
J Ethnopharmacol. 2017 Jan 04; 195:334-342.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Perilla frutescens (L.) Britt. (Lamiaceae) is a traditional herb that is consumed in East Asian countries as a traditional medicine. This traditional herb has been documented for centuries to treat various diseases such as depression, allergies, inflammation and asthma. However, the effect of Perilla frutescens on skin has not been characterized well.

AIM OF THE STUDY

The present study aimed to investigate the effect of Perilla frutescens leaves extract (PLE) on ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin.

MATERIALS AND METHODS

Human dermal fibroblasts and Skh-1 hairless mice were irradiated with UV and treated with PLE. Protein and mRNA levels of various target molecules were analyzed by western blotting and quantitative RT-PCR, respectively. Histological changes of mouse skin were analyzed by H&E staining. To elucidate underlying mechanism of PLE, activator protein-1 (AP-1) DNA binding assay and the measurement of reactive oxygen species (ROS) were performed.

RESULTS

PLE significantly inhibited basal and UV-induced MMP-1 and MMP-3 expression dose-dependently, and also decreased UV-induced phosphorylation of extracellular signal-regulated kinases and c-Jun N-terminal kinases. This inhibitory effects of PLE on MMP-1 and MMP-3 were mediated by reduction of ROS generation and AP-1 DNA binding activity induced by UV. Furthermore, PLE promoted type I procollagen production irrespective of UV irradiation. In the UV-irradiated animal model, PLE significantly reduced epidermal skin thickness and MMP-13 expression induced by UV.

CONCLUSION

Our results demonstrate that PLE has the protective effect against UV-induced dermal matrix damage. Therefore, we suggest that PLE can be a potential agent for prevention of skin aging.

Authors+Show Affiliations

Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea.Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea.Korea Food Research Institute, Seongnam-si, Kyeonggi-do 463-746, Korea.Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea.Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea.Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea. Electronic address: ivymed27@snu.ac.kr.Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea; SNU Institute on Aging, 101, Daehak-ro Jongno-gu, Seoul, Korea. Electronic address: jhchung@snu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27888134

Citation

Bae, Jung-Soo, et al. "Perilla Frutescens Leaves Extract Ameliorates Ultraviolet Radiation-induced Extracellular Matrix Damage in Human Dermal Fibroblasts and Hairless Mice Skin." Journal of Ethnopharmacology, vol. 195, 2017, pp. 334-342.
Bae JS, Han M, Shin HS, et al. Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin. J Ethnopharmacol. 2017;195:334-342.
Bae, J. S., Han, M., Shin, H. S., Kim, M. K., Shin, C. Y., Lee, D. H., & Chung, J. H. (2017). Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin. Journal of Ethnopharmacology, 195, 334-342. https://doi.org/10.1016/j.jep.2016.11.039
Bae JS, et al. Perilla Frutescens Leaves Extract Ameliorates Ultraviolet Radiation-induced Extracellular Matrix Damage in Human Dermal Fibroblasts and Hairless Mice Skin. J Ethnopharmacol. 2017 Jan 4;195:334-342. PubMed PMID: 27888134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin. AU - Bae,Jung-Soo, AU - Han,Mira, AU - Shin,Hee Soon, AU - Kim,Min-Kyoung, AU - Shin,Chang-Yup, AU - Lee,Dong Hun, AU - Chung,Jin Ho, Y1 - 2016/11/22/ PY - 2016/07/18/received PY - 2016/10/31/revised PY - 2016/11/21/accepted PY - 2016/11/27/pubmed PY - 2017/7/4/medline PY - 2016/11/27/entrez KW - Matrix metalloproteinases KW - Perilla frutescens leaves extract KW - Photoaging KW - Reactive oxygen species KW - Type I collagen KW - Ultraviolet SP - 334 EP - 342 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 195 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Perilla frutescens (L.) Britt. (Lamiaceae) is a traditional herb that is consumed in East Asian countries as a traditional medicine. This traditional herb has been documented for centuries to treat various diseases such as depression, allergies, inflammation and asthma. However, the effect of Perilla frutescens on skin has not been characterized well. AIM OF THE STUDY: The present study aimed to investigate the effect of Perilla frutescens leaves extract (PLE) on ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin. MATERIALS AND METHODS: Human dermal fibroblasts and Skh-1 hairless mice were irradiated with UV and treated with PLE. Protein and mRNA levels of various target molecules were analyzed by western blotting and quantitative RT-PCR, respectively. Histological changes of mouse skin were analyzed by H&E staining. To elucidate underlying mechanism of PLE, activator protein-1 (AP-1) DNA binding assay and the measurement of reactive oxygen species (ROS) were performed. RESULTS: PLE significantly inhibited basal and UV-induced MMP-1 and MMP-3 expression dose-dependently, and also decreased UV-induced phosphorylation of extracellular signal-regulated kinases and c-Jun N-terminal kinases. This inhibitory effects of PLE on MMP-1 and MMP-3 were mediated by reduction of ROS generation and AP-1 DNA binding activity induced by UV. Furthermore, PLE promoted type I procollagen production irrespective of UV irradiation. In the UV-irradiated animal model, PLE significantly reduced epidermal skin thickness and MMP-13 expression induced by UV. CONCLUSION: Our results demonstrate that PLE has the protective effect against UV-induced dermal matrix damage. Therefore, we suggest that PLE can be a potential agent for prevention of skin aging. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27888134/Perilla_frutescens_leaves_extract_ameliorates_ultraviolet_radiation_induced_extracellular_matrix_damage_in_human_dermal_fibroblasts_and_hairless_mice_skin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)32026-8 DB - PRIME DP - Unbound Medicine ER -