Tags

Type your tag names separated by a space and hit enter

Genetic and transcriptional study of glutathione metabolism in Oenococcus oeni.
Int J Food Microbiol 2017; 242:61-69IJ

Abstract

Although Oenococcus oeni is the main species that is responsible for malolactic fermentation (MLF), harsh wine conditions can limit its performance. Although several mechanisms underlying the response to stress have been studied in this species, little is known regarding the cellular systems that protect against oxidative stress in other bacteria, such as glutathione (GSH). O. oeni cannot synthesize GSH but contains several genes related to its utilization. In this study, the relative expression (RE) of the seven genes involved in the GSH redox system found in O. oeni PSU-1 (gshR, gpo, three glutaredoxin-like genes and two subunits of an hypothetical transporter) has been measured. The study was performed using three strains, with each exhibiting a different GSH uptake capacity. The strains were grown in a stress-adaptation medium supplemented with 5mM GSH and under different adaptation stress conditions (pH4 and 6% ethanol). The RE showed that only some of these genes, including one for a possible glutaredoxin (OEOE_RS04215) and cydC for a subunit of a putative GSH transporter (OEOE_RS1995), responded to the addition of GSH. The presence of ethanol had a relevant effect on gene expression. Among the studied genes, the one for a NrdH-redoxin (OEOE_RS00645) showed a common response to ethanol in the strains, being over-expressed when grown with GSH. In most cases, the transcriptional changes were more evident for the strain with a higher capacity of GSH uptake. Malolactic performance of the three strains after pre-adaptation was evaluated in wine-like media (12% ethanol and pH3.4). It was observed that the addition of GSH during pre-adaptation growth had a protective role in the cells exposed to low pH and ethanol, resulting in a quicker MLF.

Authors+Show Affiliations

Grup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel·lí Domingo, 1, 43007 Tarragona, Catalonia, Spain.Grup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel·lí Domingo, 1, 43007 Tarragona, Catalonia, Spain.Grup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel·lí Domingo, 1, 43007 Tarragona, Catalonia, Spain.Grup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel·lí Domingo, 1, 43007 Tarragona, Catalonia, Spain. Electronic address: cristina.reguant@urv.cat.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27889506

Citation

Margalef-Català, Mar, et al. "Genetic and Transcriptional Study of Glutathione Metabolism in Oenococcus Oeni." International Journal of Food Microbiology, vol. 242, 2017, pp. 61-69.
Margalef-Català M, Araque I, Bordons A, et al. Genetic and transcriptional study of glutathione metabolism in Oenococcus oeni. Int J Food Microbiol. 2017;242:61-69.
Margalef-Català, M., Araque, I., Bordons, A., & Reguant, C. (2017). Genetic and transcriptional study of glutathione metabolism in Oenococcus oeni. International Journal of Food Microbiology, 242, pp. 61-69. doi:10.1016/j.ijfoodmicro.2016.11.013.
Margalef-Català M, et al. Genetic and Transcriptional Study of Glutathione Metabolism in Oenococcus Oeni. Int J Food Microbiol. 2017 Feb 2;242:61-69. PubMed PMID: 27889506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and transcriptional study of glutathione metabolism in Oenococcus oeni. AU - Margalef-Català,Mar, AU - Araque,Isabel, AU - Bordons,Albert, AU - Reguant,Cristina, Y1 - 2016/11/16/ PY - 2016/03/15/received PY - 2016/11/08/revised PY - 2016/11/14/accepted PY - 2016/11/28/pubmed PY - 2017/4/19/medline PY - 2016/11/28/entrez KW - Glutaredoxin KW - Glutathione KW - Malolactic fermentation KW - NdrH KW - Oenococcus oeni KW - qPCR SP - 61 EP - 69 JF - International journal of food microbiology JO - Int. J. Food Microbiol. VL - 242 N2 - Although Oenococcus oeni is the main species that is responsible for malolactic fermentation (MLF), harsh wine conditions can limit its performance. Although several mechanisms underlying the response to stress have been studied in this species, little is known regarding the cellular systems that protect against oxidative stress in other bacteria, such as glutathione (GSH). O. oeni cannot synthesize GSH but contains several genes related to its utilization. In this study, the relative expression (RE) of the seven genes involved in the GSH redox system found in O. oeni PSU-1 (gshR, gpo, three glutaredoxin-like genes and two subunits of an hypothetical transporter) has been measured. The study was performed using three strains, with each exhibiting a different GSH uptake capacity. The strains were grown in a stress-adaptation medium supplemented with 5mM GSH and under different adaptation stress conditions (pH4 and 6% ethanol). The RE showed that only some of these genes, including one for a possible glutaredoxin (OEOE_RS04215) and cydC for a subunit of a putative GSH transporter (OEOE_RS1995), responded to the addition of GSH. The presence of ethanol had a relevant effect on gene expression. Among the studied genes, the one for a NrdH-redoxin (OEOE_RS00645) showed a common response to ethanol in the strains, being over-expressed when grown with GSH. In most cases, the transcriptional changes were more evident for the strain with a higher capacity of GSH uptake. Malolactic performance of the three strains after pre-adaptation was evaluated in wine-like media (12% ethanol and pH3.4). It was observed that the addition of GSH during pre-adaptation growth had a protective role in the cells exposed to low pH and ethanol, resulting in a quicker MLF. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/27889506/Genetic_and_transcriptional_study_of_glutathione_metabolism_in_Oenococcus_oeni_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(16)30610-9 DB - PRIME DP - Unbound Medicine ER -