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Identification of caleosin and oleosin in oil bodies of pine pollen.
Plant Physiol Biochem. 2017 Feb; 111:20-29.PP

Abstract

Unique proteins including steroleosin, caleosin, oleosin-L, and oleosin-G have been identified in seed oil bodies of pine (Pinus massoniana). In this study, mature pollen grains with wing-like bladders were collected from pine (Pinus elliottii). Ultrastructural studies showed that oil bodies were present in pollen grains, but not the attached bladders, and the presence of oil bodies was further confirmed by fluorescent staining with BODIPY 493/503. Stable oil bodies were successfully purified from pine pollen grains, and analyzed to be mainly composed of triacylglycerols. Putative oleosin and caleosin in pine pollen oil bodies were detected by immunoassaying with antibodies against sesame seed caleosin and lily pollen oleosin. Complete cDNA fragments encoding these two pollen oil-body proteins were obtained by PCR cloning. Sequence alignment showed that pine pollen caleosin (27 kDa) was highly homologous to pine seed caleosin (28 kDa) except for the lack of an appendix of eight residues at the C-terminus in accord with the 1 kDa difference in their molecular masses. Pine pollen oleosin (15 kDa) was highly homologous to pine seed oleosin-G (14 kDa) except for an insertion of eight residues at the N-terminus in accord with the 1 kDa difference in their molecular masses.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan.Department of Forestry and Natural Resources, National Chiayi University, Chiayi 600, Taiwan.Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan. Electronic address: villy@nmmba.gov.tw.Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan. Electronic address: TCTZEN@dragon.nchu.edu.tw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27889638

Citation

Pasaribu, Buntora, et al. "Identification of Caleosin and Oleosin in Oil Bodies of Pine Pollen." Plant Physiology and Biochemistry : PPB, vol. 111, 2017, pp. 20-29.
Pasaribu B, Chen CS, Liao YK, et al. Identification of caleosin and oleosin in oil bodies of pine pollen. Plant Physiol Biochem. 2017;111:20-29.
Pasaribu, B., Chen, C. S., Liao, Y. K., Jiang, P. L., & Tzen, J. T. C. (2017). Identification of caleosin and oleosin in oil bodies of pine pollen. Plant Physiology and Biochemistry : PPB, 111, 20-29. https://doi.org/10.1016/j.plaphy.2016.11.010
Pasaribu B, et al. Identification of Caleosin and Oleosin in Oil Bodies of Pine Pollen. Plant Physiol Biochem. 2017;111:20-29. PubMed PMID: 27889638.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of caleosin and oleosin in oil bodies of pine pollen. AU - Pasaribu,Buntora, AU - Chen,Chii-Shiarng, AU - Liao,Yue Ken, AU - Jiang,Pei-Luen, AU - Tzen,Jason T C, Y1 - 2016/11/18/ PY - 2016/09/12/received PY - 2016/11/14/revised PY - 2016/11/17/accepted PY - 2016/11/28/pubmed PY - 2017/5/2/medline PY - 2016/11/28/entrez KW - Caleosin KW - Oil body KW - Oleosin KW - Pine KW - Pollen SP - 20 EP - 29 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 111 N2 - Unique proteins including steroleosin, caleosin, oleosin-L, and oleosin-G have been identified in seed oil bodies of pine (Pinus massoniana). In this study, mature pollen grains with wing-like bladders were collected from pine (Pinus elliottii). Ultrastructural studies showed that oil bodies were present in pollen grains, but not the attached bladders, and the presence of oil bodies was further confirmed by fluorescent staining with BODIPY 493/503. Stable oil bodies were successfully purified from pine pollen grains, and analyzed to be mainly composed of triacylglycerols. Putative oleosin and caleosin in pine pollen oil bodies were detected by immunoassaying with antibodies against sesame seed caleosin and lily pollen oleosin. Complete cDNA fragments encoding these two pollen oil-body proteins were obtained by PCR cloning. Sequence alignment showed that pine pollen caleosin (27 kDa) was highly homologous to pine seed caleosin (28 kDa) except for the lack of an appendix of eight residues at the C-terminus in accord with the 1 kDa difference in their molecular masses. Pine pollen oleosin (15 kDa) was highly homologous to pine seed oleosin-G (14 kDa) except for an insertion of eight residues at the N-terminus in accord with the 1 kDa difference in their molecular masses. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27889638/Identification_of_caleosin_and_oleosin_in_oil_bodies_of_pine_pollen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30440-5 DB - PRIME DP - Unbound Medicine ER -