Tags

Type your tag names separated by a space and hit enter

Relevant variations and neuroprotecive effect of hydrogen sulfide in a rat glaucoma model.
Neuroscience. 2017 01 26; 341:27-41.N

Abstract

Glaucoma is an irreversible and blinding neurodegenerative disease of the eye, and is characterized by progressive loss of retinal ganglion cells (RGCs). Since endogenous hydrogen sulfide (H2S) was reported to be involved in neurodegeneration in the central nervous system, the authors aimed to develop a chronic ocular hypertension (COH) rat model simulating glaucoma and therein test the H2S level together with the retinal protein expressions of related synthases, and further investigated the effect of exogenous H2S supplement on RGC survival. COH rat model was induced by cross-linking hydrogel injection into anterior chamber, and the performance of the model was assessed by intraocular pressure (IOP) measurement, RGC counting and retinal morphological analysis. Endogenous H2S level was detected along with the retinal protein expressions of H2S-related synthases cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST) in the COH rats. Retinal H2S level and RGC survival were evaluated again after NaHS (a H2S donor) treatment in the COH rats. The results showed that the COH model succeeded in simulating glaucoma features, and retinal H2S level decreased significantly when the retinal protein expressions of CBS, CSE and 3-MST were downregulated generally in the COH rats. Furthermore, the decrease of retinal H2S level and loss of RGCs were both improved by NaHS treatment in experimental glaucoma, without obvious variation of IOP. Our study revealed that the intracameral injection of cross-linking hydrogel worked efficiently in modeling glaucoma, and H2S had protective effect on RGCs and might be involved in the pathological mechanism of glaucomatous neuropathy.

Authors+Show Affiliations

Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China. Electronic address: guolei607@126.com.Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China.Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China. Electronic address: yszhong68@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27890826

Citation

Huang, Shouyue, et al. "Relevant Variations and Neuroprotecive Effect of Hydrogen Sulfide in a Rat Glaucoma Model." Neuroscience, vol. 341, 2017, pp. 27-41.
Huang S, Huang P, Liu X, et al. Relevant variations and neuroprotecive effect of hydrogen sulfide in a rat glaucoma model. Neuroscience. 2017;341:27-41.
Huang, S., Huang, P., Liu, X., Lin, Z., Wang, J., Xu, S., Guo, L., Leung, C. K., & Zhong, Y. (2017). Relevant variations and neuroprotecive effect of hydrogen sulfide in a rat glaucoma model. Neuroscience, 341, 27-41. https://doi.org/10.1016/j.neuroscience.2016.11.019
Huang S, et al. Relevant Variations and Neuroprotecive Effect of Hydrogen Sulfide in a Rat Glaucoma Model. Neuroscience. 2017 01 26;341:27-41. PubMed PMID: 27890826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relevant variations and neuroprotecive effect of hydrogen sulfide in a rat glaucoma model. AU - Huang,Shouyue, AU - Huang,Ping, AU - Liu,Xiaohong, AU - Lin,Zhongjing, AU - Wang,Jing, AU - Xu,Shuo, AU - Guo,Lei, AU - Leung,Christopher Kai-Shun, AU - Zhong,Yisheng, Y1 - 2016/11/24/ PY - 2016/06/21/received PY - 2016/11/12/revised PY - 2016/11/16/accepted PY - 2016/11/29/pubmed PY - 2017/7/4/medline PY - 2016/11/29/entrez KW - chronic ocular hypertension KW - glaucomatous neuropathy KW - hydrogel KW - hydrogen sulfide KW - retinal ganglion cells SP - 27 EP - 41 JF - Neuroscience JO - Neuroscience VL - 341 N2 - Glaucoma is an irreversible and blinding neurodegenerative disease of the eye, and is characterized by progressive loss of retinal ganglion cells (RGCs). Since endogenous hydrogen sulfide (H2S) was reported to be involved in neurodegeneration in the central nervous system, the authors aimed to develop a chronic ocular hypertension (COH) rat model simulating glaucoma and therein test the H2S level together with the retinal protein expressions of related synthases, and further investigated the effect of exogenous H2S supplement on RGC survival. COH rat model was induced by cross-linking hydrogel injection into anterior chamber, and the performance of the model was assessed by intraocular pressure (IOP) measurement, RGC counting and retinal morphological analysis. Endogenous H2S level was detected along with the retinal protein expressions of H2S-related synthases cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST) in the COH rats. Retinal H2S level and RGC survival were evaluated again after NaHS (a H2S donor) treatment in the COH rats. The results showed that the COH model succeeded in simulating glaucoma features, and retinal H2S level decreased significantly when the retinal protein expressions of CBS, CSE and 3-MST were downregulated generally in the COH rats. Furthermore, the decrease of retinal H2S level and loss of RGCs were both improved by NaHS treatment in experimental glaucoma, without obvious variation of IOP. Our study revealed that the intracameral injection of cross-linking hydrogel worked efficiently in modeling glaucoma, and H2S had protective effect on RGCs and might be involved in the pathological mechanism of glaucomatous neuropathy. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/27890826/Relevant_variations_and_neuroprotecive_effect_of_hydrogen_sulfide_in_a_rat_glaucoma_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(16)30640-6 DB - PRIME DP - Unbound Medicine ER -