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Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice.
PLoS One. 2016; 11(11):e0166557.Plos

Abstract

The purpose of this study was to investigate the effects of leaf extract from Lithocarpus polystachyus Rehd. on type II diabetes mellitus (T2DM) and the active ingredients of this effect. In addition, this study determined, for the first time, the underlying molecular and pharmacological mechanisms of the extracts on hyperglycemia using long-term double high diet-fed and streptozotocin (STZ) induced type II diabetic mice. In the present study, leaf extract, phloridzin and trilobatin were assessed in vivo (gavage) and in vitro (non-invasive micro-test technique, NMT) in experimental T2DM mice. The biochemical parameters were measured including blood glucose and blood lipid level, liver biochemical indexes, and hepatic glycogen. The relative expression of glycometabolism-related genes was detected. The effect of leaf extracts on physiological glucose flux in liver tissue from control and T2DM mice was also investigated. Body weight of experimental T2DM mice increased significantly after the first week, but stabilized over the subsequent three weeks; body weight of all other groups did not change during the four weeks' study. After four weeks, all treatment groups decreased blood glucose, and treatment with leaf extract had numerous positive effects: a) promoted in glucose uptake in liver, b) increased synthesis of liver glycogen, c) reduced oxidative stress, d) up-regulation of glucokinase (GK), glucose transporter 2 (GLUT2), insulin receptor (IR) and insulin receptor substrate (IRS) expression in liver, e) down-regulation of glucose-6-phosphatase (G-6-P) expression, and f) ameliorated blood lipid levels. Both treatment with trilobatin or phloridzin accelerated liver glycogen synthesis, decreased oxidative stress and increased expression of GK. IRS and phosphoenolpyruvate carboxykinase (PEPCK) were both up-regulated after treatment with trilobatin. Expression of GLUT2, PEPCK and G-6-P were also increased in liver tissue after treatment with phloridzin. Our data indicate that leaf extract from L. polystachyus Rehd. has a preferable hypoglycemic effects than trilobatin or phloridzin alone. Leaf extract significantly increased glucose uptake and hepatic glycogen synthesis while also inducing a decline of hepatic gluconeogenesis and oxidative stress in T2DM mice. From this study, we draw conclusions that L. polystachyus promoted glycogen synthesis in T2DM mice, and that the active compounds were not only the trilobatin or phloridzin.

Authors+Show Affiliations

College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. Guangxi Forestry Research Institute, Nanning, Guangxi, China.College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.Food Engineering Department, Universidad del Valle, Cali, Valle del Cauca, Colombia.Agricultural and Biological Engineering Department, Institute of Agricultural and Food Science, University of Florida, Gainesville, Florida, United States of America.College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27893760

Citation

Wang, Jinfei, et al. "Leaf Extract From Lithocarpus Polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice." PloS One, vol. 11, no. 11, 2016, pp. e0166557.
Wang J, Huang Y, Li K, et al. Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice. PLoS One. 2016;11(11):e0166557.
Wang, J., Huang, Y., Li, K., Chen, Y., Vanegas, D., McLamore, E. S., & Shen, Y. (2016). Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice. PloS One, 11(11), e0166557. https://doi.org/10.1371/journal.pone.0166557
Wang J, et al. Leaf Extract From Lithocarpus Polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice. PLoS One. 2016;11(11):e0166557. PubMed PMID: 27893760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice. AU - Wang,Jinfei, AU - Huang,Yumin, AU - Li,Kaixiang, AU - Chen,Yingying, AU - Vanegas,Diana, AU - McLamore,Eric Scott, AU - Shen,Yingbai, Y1 - 2016/11/28/ PY - 2016/05/28/received PY - 2016/10/31/accepted PY - 2016/11/29/entrez PY - 2016/11/29/pubmed PY - 2017/6/28/medline SP - e0166557 EP - e0166557 JF - PloS one JO - PLoS One VL - 11 IS - 11 N2 - The purpose of this study was to investigate the effects of leaf extract from Lithocarpus polystachyus Rehd. on type II diabetes mellitus (T2DM) and the active ingredients of this effect. In addition, this study determined, for the first time, the underlying molecular and pharmacological mechanisms of the extracts on hyperglycemia using long-term double high diet-fed and streptozotocin (STZ) induced type II diabetic mice. In the present study, leaf extract, phloridzin and trilobatin were assessed in vivo (gavage) and in vitro (non-invasive micro-test technique, NMT) in experimental T2DM mice. The biochemical parameters were measured including blood glucose and blood lipid level, liver biochemical indexes, and hepatic glycogen. The relative expression of glycometabolism-related genes was detected. The effect of leaf extracts on physiological glucose flux in liver tissue from control and T2DM mice was also investigated. Body weight of experimental T2DM mice increased significantly after the first week, but stabilized over the subsequent three weeks; body weight of all other groups did not change during the four weeks' study. After four weeks, all treatment groups decreased blood glucose, and treatment with leaf extract had numerous positive effects: a) promoted in glucose uptake in liver, b) increased synthesis of liver glycogen, c) reduced oxidative stress, d) up-regulation of glucokinase (GK), glucose transporter 2 (GLUT2), insulin receptor (IR) and insulin receptor substrate (IRS) expression in liver, e) down-regulation of glucose-6-phosphatase (G-6-P) expression, and f) ameliorated blood lipid levels. Both treatment with trilobatin or phloridzin accelerated liver glycogen synthesis, decreased oxidative stress and increased expression of GK. IRS and phosphoenolpyruvate carboxykinase (PEPCK) were both up-regulated after treatment with trilobatin. Expression of GLUT2, PEPCK and G-6-P were also increased in liver tissue after treatment with phloridzin. Our data indicate that leaf extract from L. polystachyus Rehd. has a preferable hypoglycemic effects than trilobatin or phloridzin alone. Leaf extract significantly increased glucose uptake and hepatic glycogen synthesis while also inducing a decline of hepatic gluconeogenesis and oxidative stress in T2DM mice. From this study, we draw conclusions that L. polystachyus promoted glycogen synthesis in T2DM mice, and that the active compounds were not only the trilobatin or phloridzin. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27893760/Leaf_Extract_from_Lithocarpus_polystachyus_Rehd__Promote_Glycogen_Synthesis_in_T2DM_Mice_ L2 - https://dx.plos.org/10.1371/journal.pone.0166557 DB - PRIME DP - Unbound Medicine ER -