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Increased CD4+CD45RA-FoxP3low cells alter the balance between Treg and Th17 cells in colitis mice.
World J Gastroenterol 2016; 22(42):9356-9367WJ

Abstract

AIM

To investigate the role of regulatory T cell (Treg) subsets in the balance between Treg and T helper 17 (Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.

METHODS

Treg cells, Treg cell subsets, Th17 cells, and CD4+CD25+FoxP3+IL-17+ cells from the lamina propria of colon (LPC) and other ulcerative colitis (UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3 (FoxP3), interleukin 17A (IL-17A), and RORC mRNA levels were assessed by real-time PCR, while interleukin-10 (IL-10) and IL-17A levels were detected with a Cytometric Beads Array.

RESULTS

In peripheral blood monocytes (PBMC), mesenteric lymph node (MLN), lamina propria of jejunum (LPJ) and LPC from UC mice, Treg cell numbers were increased (P < 0.05), and FoxP3 and IL-10 mRNA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17A levels in PBMC, LPJ, and serum. The number of FrI subset cells (CD4+CD45RA+FoxP3low) was increased in the spleen, MLN, LPJ, and LPC. FrII subset cells (CD4+CD45RA-FoxP3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of FrIII cells (CD4+CD45RA-FoxP3low) and CD4+CD25+FoxP3+IL-17A+ cells was increased. FoxP3 mRNA levels in CD4+CD45RA-FoxP3low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17A and RORC mRNA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP3high, and CD4+CD45RA-FoxP3low cells was higher in LPC relative to other tissues.

CONCLUSION

Increased numbers of CD4+CD45RA-FoxP3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.

Authors+Show Affiliations

Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27895423

Citation

Ma, Ya-Hui, et al. "Increased CD4+CD45RA-FoxP3low Cells Alter the Balance Between Treg and Th17 Cells in Colitis Mice." World Journal of Gastroenterology, vol. 22, no. 42, 2016, pp. 9356-9367.
Ma YH, Zhang J, Chen X, et al. Increased CD4+CD45RA-FoxP3low cells alter the balance between Treg and Th17 cells in colitis mice. World J Gastroenterol. 2016;22(42):9356-9367.
Ma, Y. H., Zhang, J., Chen, X., Xie, Y. F., Pang, Y. H., & Liu, X. J. (2016). Increased CD4+CD45RA-FoxP3low cells alter the balance between Treg and Th17 cells in colitis mice. World Journal of Gastroenterology, 22(42), pp. 9356-9367.
Ma YH, et al. Increased CD4+CD45RA-FoxP3low Cells Alter the Balance Between Treg and Th17 Cells in Colitis Mice. World J Gastroenterol. 2016 Nov 14;22(42):9356-9367. PubMed PMID: 27895423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased CD4+CD45RA-FoxP3low cells alter the balance between Treg and Th17 cells in colitis mice. AU - Ma,Ya-Hui, AU - Zhang,Jie, AU - Chen,Xue, AU - Xie,You-Fu, AU - Pang,Yan-Hua, AU - Liu,Xin-Juan, PY - 2016/05/10/received PY - 2016/08/05/revised PY - 2016/08/19/accepted PY - 2016/11/30/entrez PY - 2016/11/30/pubmed PY - 2017/4/25/medline KW - Regulatory T cells KW - T helper 17 cells KW - Treg cells subsets KW - Ulcerative colitis SP - 9356 EP - 9367 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 22 IS - 42 N2 - AIM: To investigate the role of regulatory T cell (Treg) subsets in the balance between Treg and T helper 17 (Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis. METHODS: Treg cells, Treg cell subsets, Th17 cells, and CD4+CD25+FoxP3+IL-17+ cells from the lamina propria of colon (LPC) and other ulcerative colitis (UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3 (FoxP3), interleukin 17A (IL-17A), and RORC mRNA levels were assessed by real-time PCR, while interleukin-10 (IL-10) and IL-17A levels were detected with a Cytometric Beads Array. RESULTS: In peripheral blood monocytes (PBMC), mesenteric lymph node (MLN), lamina propria of jejunum (LPJ) and LPC from UC mice, Treg cell numbers were increased (P < 0.05), and FoxP3 and IL-10 mRNA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17A levels in PBMC, LPJ, and serum. The number of FrI subset cells (CD4+CD45RA+FoxP3low) was increased in the spleen, MLN, LPJ, and LPC. FrII subset cells (CD4+CD45RA-FoxP3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of FrIII cells (CD4+CD45RA-FoxP3low) and CD4+CD25+FoxP3+IL-17A+ cells was increased. FoxP3 mRNA levels in CD4+CD45RA-FoxP3low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17A and RORC mRNA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP3high, and CD4+CD45RA-FoxP3low cells was higher in LPC relative to other tissues. CONCLUSION: Increased numbers of CD4+CD45RA-FoxP3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/27895423/Increased_CD4+CD45RA_FoxP3low_cells_alter_the_balance_between_Treg_and_Th17_cells_in_colitis_mice_ L2 - http://www.wjgnet.com/1007-9327/full/v22/i42/9356.htm DB - PRIME DP - Unbound Medicine ER -