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Homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and Triticum mosaic virus resistance gene Wsm3 from Thinopyrum intermedium to wheat.
Theor Appl Genet. 2017 Mar; 130(3):549-556.TA

Abstract

KEY MESSAGE

Here, we report the production of a wheat- Thinopyrum intermedium recombinant stock conferring resistance to wheat streak mosaic virus and Triticum mosaic virus. Wheat streak mosaic caused by the wheat streak mosaic virus (WSMV) is an important disease of bread wheat (Triticum aestivum) worldwide. To date, only three genes conferring resistance to WSMV have been named and two, Wsm1 and Wsm3, were derived from the distantly related wild relative Thinopyrum intermedium. Wsm3 is only available in the form of a compensating wheat-Th. intermedium whole-arm Robertsonian translocation T7BS·7S#3L. Whole-arm alien transfers usually suffer from linkage drag, which prevents their use in cultivar improvement. Here, we report ph1b-induced homoeologous recombination to shorten the Th. intermedium segment and recover a recombinant chromosome consisting of the short arm of wheat chromosome 7B, part of the long arm of 7B, and the distal 43% of the long arm derived from the Th. intermedium chromosome arm 7S#3L. The recombinant chromosome T7BS·7BL-7S#3L confers resistance to WSMV at 18 and 24 °C and also confers resistance to Triticum mosaic virus, but only at 18 °C. Wsm3 is the only gene conferring resistance to WSMV at a high temperature level of 24 °C. We also developed a user-friendly molecular marker that will allow to monitor the transfer of Wsm3 in breeding programs. Wsm3 is presently being transferred to adapted hard red winter wheat cultivars and can be used directly in wheat improvement.

Authors+Show Affiliations

Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.Agricultural Research Center-Hays, Kansas State University, Hays, KS, 67601, USA.Laboratory of Cell and Chromosome Engineering, College of Life Sciences, Henan Agricultural University, 450002, Zhengzhou, Henan, People's Republic of China.Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA. friebe@ksu.edu.Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27900400

Citation

Danilova, Tatiana V., et al. "Homoeologous Recombination-based Transfer and Molecular Cytogenetic Mapping of a Wheat Streak Mosaic Virus and Triticum Mosaic Virus Resistance Gene Wsm3 From Thinopyrum Intermedium to Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 3, 2017, pp. 549-556.
Danilova TV, Zhang G, Liu W, et al. Homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and Triticum mosaic virus resistance gene Wsm3 from Thinopyrum intermedium to wheat. Theor Appl Genet. 2017;130(3):549-556.
Danilova, T. V., Zhang, G., Liu, W., Friebe, B., & Gill, B. S. (2017). Homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and Triticum mosaic virus resistance gene Wsm3 from Thinopyrum intermedium to wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(3), 549-556. https://doi.org/10.1007/s00122-016-2834-8
Danilova TV, et al. Homoeologous Recombination-based Transfer and Molecular Cytogenetic Mapping of a Wheat Streak Mosaic Virus and Triticum Mosaic Virus Resistance Gene Wsm3 From Thinopyrum Intermedium to Wheat. Theor Appl Genet. 2017;130(3):549-556. PubMed PMID: 27900400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and Triticum mosaic virus resistance gene Wsm3 from Thinopyrum intermedium to wheat. AU - Danilova,Tatiana V, AU - Zhang,Guorong, AU - Liu,Wenxuan, AU - Friebe,Bernd, AU - Gill,Bikram S, Y1 - 2016/11/29/ PY - 2016/10/07/received PY - 2016/11/18/accepted PY - 2016/12/3/pubmed PY - 2017/3/7/medline PY - 2016/12/1/entrez SP - 549 EP - 556 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 3 N2 - KEY MESSAGE: Here, we report the production of a wheat- Thinopyrum intermedium recombinant stock conferring resistance to wheat streak mosaic virus and Triticum mosaic virus. Wheat streak mosaic caused by the wheat streak mosaic virus (WSMV) is an important disease of bread wheat (Triticum aestivum) worldwide. To date, only three genes conferring resistance to WSMV have been named and two, Wsm1 and Wsm3, were derived from the distantly related wild relative Thinopyrum intermedium. Wsm3 is only available in the form of a compensating wheat-Th. intermedium whole-arm Robertsonian translocation T7BS·7S#3L. Whole-arm alien transfers usually suffer from linkage drag, which prevents their use in cultivar improvement. Here, we report ph1b-induced homoeologous recombination to shorten the Th. intermedium segment and recover a recombinant chromosome consisting of the short arm of wheat chromosome 7B, part of the long arm of 7B, and the distal 43% of the long arm derived from the Th. intermedium chromosome arm 7S#3L. The recombinant chromosome T7BS·7BL-7S#3L confers resistance to WSMV at 18 and 24 °C and also confers resistance to Triticum mosaic virus, but only at 18 °C. Wsm3 is the only gene conferring resistance to WSMV at a high temperature level of 24 °C. We also developed a user-friendly molecular marker that will allow to monitor the transfer of Wsm3 in breeding programs. Wsm3 is presently being transferred to adapted hard red winter wheat cultivars and can be used directly in wheat improvement. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/27900400/Homoeologous_recombination_based_transfer_and_molecular_cytogenetic_mapping_of_a_wheat_streak_mosaic_virus_and_Triticum_mosaic_virus_resistance_gene_Wsm3_from_Thinopyrum_intermedium_to_wheat_ L2 - https://dx.doi.org/10.1007/s00122-016-2834-8 DB - PRIME DP - Unbound Medicine ER -