Tags

Type your tag names separated by a space and hit enter

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping-by-Sequencing.
Plant Genome. 2016 11; 9(3)PG

Abstract

Basal stalk rot (BSR), caused by the ascomycete fungus (Lib.) de Bary, is a serious disease of sunflower (L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 × RHA 439. A genotyping-by-sequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401.36 cM. The RILs were tested in five environments (locations and years) for resistance to BSR. Quantitative trait loci were identified in each environment separately and also with integrated data across environments. A total of six QTL were identified in all five environments: one of each on LGs 4, 9, 10, 11, 16, and 17. The most significant QTL, and , were identified at multiple environments on LGs 10 and 17, explaining 31.6 and 20.2% of the observed phenotypic variance, respectively. The remaining four QTL, , , , and , were detected in only one environment on LGs 4, 9, 11, and 16, respectively. Each of these QTL explains between 6.4 and 10.5% of the observed phenotypic variation in the RIL population. Alleles conferring increased resistance were contributed by both parents. The potential of the and in marker-assisted selection (MAS) breeding are discussed.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

27902793

Citation

Talukder, Zahirul I., et al. "SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower Using Genotyping-by-Sequencing." The Plant Genome, vol. 9, no. 3, 2016.
Talukder ZI, Seiler GJ, Song Q, et al. SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping-by-Sequencing. Plant Genome. 2016;9(3).
Talukder, Z. I., Seiler, G. J., Song, Q., Ma, G., & Qi, L. (2016). SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping-by-Sequencing. The Plant Genome, 9(3). https://doi.org/10.3835/plantgenome2016.03.0035
Talukder ZI, et al. SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower Using Genotyping-by-Sequencing. Plant Genome. 2016;9(3) PubMed PMID: 27902793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping-by-Sequencing. AU - Talukder,Zahirul I, AU - Seiler,Gerald J, AU - Song,Qijian, AU - Ma,Guojia, AU - Qi,Lili, PY - 2016/12/1/entrez PY - 2016/12/3/pubmed PY - 2018/8/24/medline JF - The plant genome JO - Plant Genome VL - 9 IS - 3 N2 - Basal stalk rot (BSR), caused by the ascomycete fungus (Lib.) de Bary, is a serious disease of sunflower (L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 × RHA 439. A genotyping-by-sequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401.36 cM. The RILs were tested in five environments (locations and years) for resistance to BSR. Quantitative trait loci were identified in each environment separately and also with integrated data across environments. A total of six QTL were identified in all five environments: one of each on LGs 4, 9, 10, 11, 16, and 17. The most significant QTL, and , were identified at multiple environments on LGs 10 and 17, explaining 31.6 and 20.2% of the observed phenotypic variance, respectively. The remaining four QTL, , , , and , were detected in only one environment on LGs 4, 9, 11, and 16, respectively. Each of these QTL explains between 6.4 and 10.5% of the observed phenotypic variation in the RIL population. Alleles conferring increased resistance were contributed by both parents. The potential of the and in marker-assisted selection (MAS) breeding are discussed. SN - 1940-3372 UR - https://www.unboundmedicine.com/medline/citation/27902793/SNP_Discovery_and_QTL_Mapping_of_Sclerotinia_Basal_Stalk_Rot_Resistance_in_Sunflower_using_Genotyping_by_Sequencing_ L2 - https://doi.org/10.3835/plantgenome2016.03.0035 DB - PRIME DP - Unbound Medicine ER -