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Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease.
J Nutr. 2017 01; 147(1):52-60.JN

Abstract

BACKGROUND

As the prevalence of nonalcoholic fatty liver disease (NAFLD), including steatosis and nonalcoholic steatohepatitis, is increasing, novel dietary approaches are required for the prevention and treatment of NAFLD.

OBJECTIVE

We evaluated the potential of mung bean protein isolate (MuPI) to prevent NAFLD progression.

METHODS

In Expts. 1 and 2, the hepatic triglyceride (TG) concentration was compared between 8-wk-old male mice fed a high-fat diet (61% of energy from fat) containing casein, MuPI, and soy protein isolate and an MuPI-constituent amino acid mixture as a source of amino acids (18% of energy) for 4 wk. In Expt. 3, hepatic fatty acid synthase (Fasn) expression was evaluated in 8-wk-old male Fasn-promoter-reporter mice fed a casein- or MuPI-containing high-fat diet for 20 wk. In Expt. 4, hepatic fibrosis was examined in 8-wk-old male mice fed an atherogenic diet (61% of energy from fat, containing 1.3 g cholesterol/100 g diet) containing casein or MuPI (18% of energy) as a protein source for 20 wk.

RESULTS

In the high fat-diet mice, the hepatic TG concentration in the MuPI group decreased by 66% and 47% in Expt. 1 compared with the casein group (P < 0.001) and the soy protein isolate group (P = 0.001), respectively, and decreased by 56% in Expt. 2 compared with the casein group (P = 0.011). However, there was no difference between the MuPI-constituent amino acid mixture and casein groups in Expt. 2. In Expt. 3, Fasn-promoter-reporter activity and hepatic TG concentration were lower in the MuPI group than in those fed casein (P < 0.05). In Expt. 4, in mice fed an atherogenic diet, hepatic fibrosis was not induced in the MuPI group, whereas it developed overtly in the casein group.

CONCLUSION

MuPI potently reduced hepatic lipid accumulation in mice and may be a potential foodstuff to prevent NAFLD onset and progression.

Authors+Show Affiliations

Metabolism and Nutrition Research Unit, Institute for Frontier Science Initiative. Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.Metabolism and Nutrition Research Unit, Institute for Frontier Science Initiative. Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.Division of Diabetes and Endocrinology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.Division of Diabetes and Endocrinology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan. Division of Medical Chemistry, Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Hyogo, Japan.Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan; and.Health and Nutrition Research Department, Research and Development Division for Future Creation, Fuji Oil Co., Ltd., Tsukuba-mirai, Ibaraki, Japan.Health and Nutrition Research Department, Research and Development Division for Future Creation, Fuji Oil Co., Ltd., Tsukuba-mirai, Ibaraki, Japan.Department of Disease Control and Homeostasis, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.Health and Nutrition Research Department, Research and Development Division for Future Creation, Fuji Oil Co., Ltd., Tsukuba-mirai, Ibaraki, Japan.Metabolism and Nutrition Research Unit, Institute for Frontier Science Initiative, inoue-h@staff.kanazawa-u.ac.jp. Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27903831

Citation

Watanabe, Hitoshi, et al. "Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice With Diet-Induced, Nonalcoholic Fatty Liver Disease." The Journal of Nutrition, vol. 147, no. 1, 2017, pp. 52-60.
Watanabe H, Inaba Y, Kimura K, et al. Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease. J Nutr. 2017;147(1):52-60.
Watanabe, H., Inaba, Y., Kimura, K., Asahara, S. I., Kido, Y., Matsumoto, M., Motoyama, T., Tachibana, N., Kaneko, S., Kohno, M., & Inoue, H. (2017). Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease. The Journal of Nutrition, 147(1), 52-60. https://doi.org/10.3945/jn.116.231662
Watanabe H, et al. Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice With Diet-Induced, Nonalcoholic Fatty Liver Disease. J Nutr. 2017;147(1):52-60. PubMed PMID: 27903831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease. AU - Watanabe,Hitoshi, AU - Inaba,Yuka, AU - Kimura,Kumi, AU - Asahara,Shun-Ichiro, AU - Kido,Yoshiaki, AU - Matsumoto,Michihiro, AU - Motoyama,Takayasu, AU - Tachibana,Nobuhiko, AU - Kaneko,Shuichi, AU - Kohno,Mitsutaka, AU - Inoue,Hiroshi, Y1 - 2016/11/30/ PY - 2016/07/01/received PY - 2016/07/28/revised PY - 2016/11/01/accepted PY - 2016/12/3/pubmed PY - 2017/6/16/medline PY - 2016/12/2/entrez KW - de novo lipogenesis KW - hepatic fibrosis KW - hepatic steatosis KW - mung bean protein isolate KW - nonalcoholic fatty liver disease KW - soy protein isolate SP - 52 EP - 60 JF - The Journal of nutrition JO - J. Nutr. VL - 147 IS - 1 N2 - BACKGROUND: As the prevalence of nonalcoholic fatty liver disease (NAFLD), including steatosis and nonalcoholic steatohepatitis, is increasing, novel dietary approaches are required for the prevention and treatment of NAFLD. OBJECTIVE: We evaluated the potential of mung bean protein isolate (MuPI) to prevent NAFLD progression. METHODS: In Expts. 1 and 2, the hepatic triglyceride (TG) concentration was compared between 8-wk-old male mice fed a high-fat diet (61% of energy from fat) containing casein, MuPI, and soy protein isolate and an MuPI-constituent amino acid mixture as a source of amino acids (18% of energy) for 4 wk. In Expt. 3, hepatic fatty acid synthase (Fasn) expression was evaluated in 8-wk-old male Fasn-promoter-reporter mice fed a casein- or MuPI-containing high-fat diet for 20 wk. In Expt. 4, hepatic fibrosis was examined in 8-wk-old male mice fed an atherogenic diet (61% of energy from fat, containing 1.3 g cholesterol/100 g diet) containing casein or MuPI (18% of energy) as a protein source for 20 wk. RESULTS: In the high fat-diet mice, the hepatic TG concentration in the MuPI group decreased by 66% and 47% in Expt. 1 compared with the casein group (P < 0.001) and the soy protein isolate group (P = 0.001), respectively, and decreased by 56% in Expt. 2 compared with the casein group (P = 0.011). However, there was no difference between the MuPI-constituent amino acid mixture and casein groups in Expt. 2. In Expt. 3, Fasn-promoter-reporter activity and hepatic TG concentration were lower in the MuPI group than in those fed casein (P < 0.05). In Expt. 4, in mice fed an atherogenic diet, hepatic fibrosis was not induced in the MuPI group, whereas it developed overtly in the casein group. CONCLUSION: MuPI potently reduced hepatic lipid accumulation in mice and may be a potential foodstuff to prevent NAFLD onset and progression. SN - 1541-6100 UR - https://www.unboundmedicine.com/medline/citation/27903831/Dietary_Mung_Bean_Protein_Reduces_Hepatic_Steatosis_Fibrosis_and_Inflammation_in_Male_Mice_with_Diet_Induced_Nonalcoholic_Fatty_Liver_Disease_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.3945/jn.116.231662 DB - PRIME DP - Unbound Medicine ER -