Tags

Type your tag names separated by a space and hit enter

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes.
PLoS One. 2016; 11(12):e0167531.Plos

Abstract

Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-diphenyl-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2'-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis.

Authors+Show Affiliations

School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Dourados, MS, Brazil.School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Dourados, MS, Brazil.Department of Biochemistry, Federal University of São Paulo, São Paulo, SP, Brazil.Department of Biochemistry, Federal University of São Paulo, São Paulo, SP, Brazil. Center for Interdisciplinary Research Biochemistry, University of Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, Mogi das Cruzes, SP, Brazil.Federal University of Mato Grosso do Sul, Center of Biological and Health Sciences, Laboratory of Natural Products and Mass Spectrometry, Campo Grande, MS, Brazil.Agricultural College of Bragança, Polytechnic Institute of Bragança, Campus Santa Apolónia, Bragança, Portugal and Centre of Molecular and Environmental Biology, University of Minho, Campus de Gualtar, Braga, Portugal.School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Dourados, MS, Brazil.School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Dourados, MS, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27907185

Citation

Santos, Uilson P., et al. "Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract From Hancornia Speciosa Gomes." PloS One, vol. 11, no. 12, 2016, pp. e0167531.
Santos UP, Campos JF, Torquato HF, et al. Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes. PLoS One. 2016;11(12):e0167531.
Santos, U. P., Campos, J. F., Torquato, H. F., Paredes-Gamero, E. J., Carollo, C. A., Estevinho, L. M., de Picoli Souza, K., & Dos Santos, E. L. (2016). Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes. PloS One, 11(12), e0167531. https://doi.org/10.1371/journal.pone.0167531
Santos UP, et al. Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract From Hancornia Speciosa Gomes. PLoS One. 2016;11(12):e0167531. PubMed PMID: 27907185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes. AU - Santos,Uilson P, AU - Campos,Jaqueline F, AU - Torquato,Heron Fernandes V, AU - Paredes-Gamero,Edgar Julian, AU - Carollo,Carlos Alexandre, AU - Estevinho,Leticia M, AU - de Picoli Souza,Kely, AU - Dos Santos,Edson Lucas, Y1 - 2016/12/01/ PY - 2016/09/05/received PY - 2016/11/09/accepted PY - 2016/12/2/entrez PY - 2016/12/3/pubmed PY - 2017/7/1/medline SP - e0167531 EP - e0167531 JF - PloS one JO - PLoS One VL - 11 IS - 12 N2 - Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-diphenyl-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2'-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27907185/Antioxidant_Antimicrobial_and_Cytotoxic_Properties_as_Well_as_the_Phenolic_Content_of_the_Extract_from_Hancornia_speciosa_Gomes_ L2 - https://dx.plos.org/10.1371/journal.pone.0167531 DB - PRIME DP - Unbound Medicine ER -