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Developmental validation of the HomyGene19+14Y System.
Int J Legal Med. 2017 May; 131(3):605-620.IJ

Abstract

The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications. In this study, the multiplex system was validated for sensitivity, specificity, DNA mixtures, stability, precision, stutter, reproducibility, parallel tests, PCR-based conditions, and population analysis according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation guidelines. A total of 212 alleles were detected for the 18 autosomal STR loci among 528 Guangdong Han individuals, and 431 haplotypes were found for 14 Y-STRs among 452 unrelated males. The combined match probability (CMP) of the HG19+14Y System was calculated as 2.39 × 10-29. All the validation results showed that the HG19+14Y System would be a robust, reliable, highly polymorphic, and informative forensic kit.

Authors+Show Affiliations

School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China.School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.AGCU ScienTech Incorporation, Wuxi, 214174, China.HOMY GeneTech Incorporation, Foshan, 518000, China.HOMY GeneTech Incorporation, Foshan, 518000, China.HOMY GeneTech Incorporation, Foshan, 518000, China.School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China. liuchaogzf@163.com. Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China. liuchaogzf@163.com.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

27909867

Citation

Du, Weian, et al. "Developmental Validation of the HomyGene19+14Y System." International Journal of Legal Medicine, vol. 131, no. 3, 2017, pp. 605-620.
Du W, Chen L, Liu H, et al. Developmental validation of the HomyGene19+14Y System. Int J Legal Med. 2017;131(3):605-620.
Du, W., Chen, L., Liu, H., Qiu, P., Li, F., Gao, J., Zhou, Y., Wang, B., & Liu, C. (2017). Developmental validation of the HomyGene19+14Y System. International Journal of Legal Medicine, 131(3), 605-620. https://doi.org/10.1007/s00414-016-1505-2
Du W, et al. Developmental Validation of the HomyGene19+14Y System. Int J Legal Med. 2017;131(3):605-620. PubMed PMID: 27909867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental validation of the HomyGene19+14Y System. AU - Du,Weian, AU - Chen,Ling, AU - Liu,Hong, AU - Qiu,Pingming, AU - Li,Fayuan, AU - Gao,Jing, AU - Zhou,Yu, AU - Wang,Bangchao, AU - Liu,Chao, Y1 - 2016/12/01/ PY - 2016/10/08/received PY - 2016/11/22/accepted PY - 2016/12/3/pubmed PY - 2017/11/1/medline PY - 2016/12/3/entrez KW - DNA typing KW - Forensic science KW - Multiplex PCR KW - STR KW - Short tandem repeat KW - Validation SP - 605 EP - 620 JF - International journal of legal medicine JO - Int J Legal Med VL - 131 IS - 3 N2 - The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications. In this study, the multiplex system was validated for sensitivity, specificity, DNA mixtures, stability, precision, stutter, reproducibility, parallel tests, PCR-based conditions, and population analysis according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation guidelines. A total of 212 alleles were detected for the 18 autosomal STR loci among 528 Guangdong Han individuals, and 431 haplotypes were found for 14 Y-STRs among 452 unrelated males. The combined match probability (CMP) of the HG19+14Y System was calculated as 2.39 × 10-29. All the validation results showed that the HG19+14Y System would be a robust, reliable, highly polymorphic, and informative forensic kit. SN - 1437-1596 UR - https://www.unboundmedicine.com/medline/citation/27909867/Developmental_validation_of_the_HomyGene19+14Y_System_ L2 - https://dx.doi.org/10.1007/s00414-016-1505-2 DB - PRIME DP - Unbound Medicine ER -