Tags

Type your tag names separated by a space and hit enter

Calea uniflora Less. attenuates the inflammatory response to carrageenan-induced pleurisy in mice.
Int Immunopharmacol. 2017 Jan; 42:139-149.II

Abstract

Calea uniflora Less. (family Asteraceae), also named "arnica" and "erva-de-lagarto", is a native plant to the South and Southeast of Brazil. This species was used to treat rheumatism, respiratory diseases, and digestive problems in Brazilian folk medicine. In vitro studies have shown the important biological effects of C. uniflora. However no studies have focused on the mechanism of action of anti-inflammatory activity of C. uniflora. The aim of this study was to evaluate the anti-inflammatory effects of the crude extract, its fractions, and isolated compounds obtained from of C. uniflora, using mouse model of carrageenan-induced inflammation. The following inflammatory parameters: leukocyte influx, degree of exudation, myeloperoxidase (MPO) and adenosine deaminase (ADA) activities, nitric oxide metabolites (NOx), proinflammatory cytokines and phosphorylation of the p65 subunit of NF-κB (p-p65 NF-κB), and p38 mitogen-activated protein kinase (p-p38 MAPK) levels were determined. The crude extract of C. uniflora, its fractions and its isolated compounds reduced the leukocyte influx, degree of exudation, MPO and ADA activities, NOx, TNF-α, IFN-γ, MCP-1 and IL-6 levels (p<0.05). The isolated compounds reduced p-p65 NF-κB and p-p38 MAPK levels (p<0.01). This study demonstrated that C. uniflora exhibits a significant anti-inflammatory activity via inhibition of the leukocyte influx and degree of exudation. These effects were associated with a decrease in the levels of several proinflammatory mediators. The mechanism of the anti-inflammatory action of C. uniflora may be, at least in part, via the inhibition of p65 NF-κB and p38 MAPK activation by the isolated compounds.

Authors+Show Affiliations

Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Pathology, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.. Electronic address: tania.frode@ufsc.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27912150

Citation

da Rosa, Julia Salvan, et al. "Calea Uniflora Less. Attenuates the Inflammatory Response to Carrageenan-induced Pleurisy in Mice." International Immunopharmacology, vol. 42, 2017, pp. 139-149.
da Rosa JS, de Mello SV, Vicente G, et al. Calea uniflora Less. attenuates the inflammatory response to carrageenan-induced pleurisy in mice. Int Immunopharmacol. 2017;42:139-149.
da Rosa, J. S., de Mello, S. V., Vicente, G., Moon, Y. J., Daltoé, F. P., Lima, T. C., de Jesus Souza, R., Biavatti, M. W., & Fröde, T. S. (2017). Calea uniflora Less. attenuates the inflammatory response to carrageenan-induced pleurisy in mice. International Immunopharmacology, 42, 139-149. https://doi.org/10.1016/j.intimp.2016.11.029
da Rosa JS, et al. Calea Uniflora Less. Attenuates the Inflammatory Response to Carrageenan-induced Pleurisy in Mice. Int Immunopharmacol. 2017;42:139-149. PubMed PMID: 27912150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calea uniflora Less. attenuates the inflammatory response to carrageenan-induced pleurisy in mice. AU - da Rosa,Julia Salvan, AU - de Mello,Silvana Virginia Gagliotti Vigil, AU - Vicente,Geison, AU - Moon,Yeo Jim K, AU - Daltoé,Felipe Perozzo, AU - Lima,Tamires Cardoso, AU - de Jesus Souza,Rafaela, AU - Biavatti,Maique Weber, AU - Fröde,Tânia Silvia, Y1 - 2016/11/30/ PY - 2016/06/21/received PY - 2016/10/27/revised PY - 2016/11/24/accepted PY - 2016/12/3/pubmed PY - 2017/4/15/medline PY - 2016/12/3/entrez KW - Anti-inflammatory effect KW - Calea uniflora Less KW - MAPK KW - NF-κB KW - Proinflammatory cytokine KW - Proinflammatory enzyme SP - 139 EP - 149 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 42 N2 - Calea uniflora Less. (family Asteraceae), also named "arnica" and "erva-de-lagarto", is a native plant to the South and Southeast of Brazil. This species was used to treat rheumatism, respiratory diseases, and digestive problems in Brazilian folk medicine. In vitro studies have shown the important biological effects of C. uniflora. However no studies have focused on the mechanism of action of anti-inflammatory activity of C. uniflora. The aim of this study was to evaluate the anti-inflammatory effects of the crude extract, its fractions, and isolated compounds obtained from of C. uniflora, using mouse model of carrageenan-induced inflammation. The following inflammatory parameters: leukocyte influx, degree of exudation, myeloperoxidase (MPO) and adenosine deaminase (ADA) activities, nitric oxide metabolites (NOx), proinflammatory cytokines and phosphorylation of the p65 subunit of NF-κB (p-p65 NF-κB), and p38 mitogen-activated protein kinase (p-p38 MAPK) levels were determined. The crude extract of C. uniflora, its fractions and its isolated compounds reduced the leukocyte influx, degree of exudation, MPO and ADA activities, NOx, TNF-α, IFN-γ, MCP-1 and IL-6 levels (p<0.05). The isolated compounds reduced p-p65 NF-κB and p-p38 MAPK levels (p<0.01). This study demonstrated that C. uniflora exhibits a significant anti-inflammatory activity via inhibition of the leukocyte influx and degree of exudation. These effects were associated with a decrease in the levels of several proinflammatory mediators. The mechanism of the anti-inflammatory action of C. uniflora may be, at least in part, via the inhibition of p65 NF-κB and p38 MAPK activation by the isolated compounds. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/27912150/Calea_uniflora_Less__attenuates_the_inflammatory_response_to_carrageenan_induced_pleurisy_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(16)30485-4 DB - PRIME DP - Unbound Medicine ER -