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Dietary fish oil replacement by linseed oil: Effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (Puntius gonionotus) fingerlings.

Abstract

Silver barb (Puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in Asia. This study in silver barb was carried out to evaluate the effects of total or partial substitution of dietary fish oil (FO) with linseed oil (LO) on growth, nutrient utilization, whole-body composition, muscle and liver fatty acid composition. Fish (12.1±0.4g of initial body weight) were fed for 60days with five experimental iso-proteinous, iso-lipidic and iso-caloric diets in which FO (control diet) was replaced by 33.3%, 50%, 66.7% and 100% LO. Final weight, weight gain, percent weight gain, SGR decreased linearly (p<0.001) with increasing LO levels in the diets. Dietary LO substitution levels did not significantly (p>0.05) affect the feed conversion ratio (FCR), protein efficiency ratio (PER) and whole body proximate composition. Furthermore, enhanced level of LO increased α-linolenic acid (ALA; 18:3n3) and linoleic acid (LA; 18:2n6) and decreased eicosapentaenoic acid (EPA; 20:5n3) and docosahexaenoic acid (DHA; 22:6n3) in muscle and liver. To understand the molecular mechanism of long chain-polyunsaturated fatty acid (LC-PUFA) biosynthesis, we cloned and characterized the fatty acyl Δ6 desaturase (Δ6 fad) cDNA and investigated its expression in various organs/tissues following replacement of FO with LO in the diet. The full-length Δ6 fad cDNA was 2056bp encoding 444 amino acids and was widely expressed in various organs/tissues. Replacement of FO with LO increased the expression of Δ6 fad mRNA in liver, muscle and intestine but no significant difference was found in the brain.

Authors+Show Affiliations

Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India. Electronic address: ashis73in@yahoo.co.in.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27913275

Citation

Nayak, Madhusmita, et al. "Dietary Fish Oil Replacement By Linseed Oil: Effect On Growth, Nutrient Utilization, Tissue Fatty Acid Composition and Desaturase Gene Expression in Silver Barb (Puntius Gonionotus) Fingerlings." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 205, 2017, pp. 1-12.
Nayak M, Saha A, Pradhan A, et al. Dietary fish oil replacement by linseed oil: Effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (Puntius gonionotus) fingerlings. Comp Biochem Physiol B, Biochem Mol Biol. 2017;205:1-12.
Nayak, M., Saha, A., Pradhan, A., Samanta, M., & Giri, S. S. (2017). Dietary fish oil replacement by linseed oil: Effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (Puntius gonionotus) fingerlings. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 205, 1-12. https://doi.org/10.1016/j.cbpb.2016.11.009
Nayak M, et al. Dietary Fish Oil Replacement By Linseed Oil: Effect On Growth, Nutrient Utilization, Tissue Fatty Acid Composition and Desaturase Gene Expression in Silver Barb (Puntius Gonionotus) Fingerlings. Comp Biochem Physiol B, Biochem Mol Biol. 2017;205:1-12. PubMed PMID: 27913275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fish oil replacement by linseed oil: Effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (Puntius gonionotus) fingerlings. AU - Nayak,Madhusmita, AU - Saha,Ashis, AU - Pradhan,Avinash, AU - Samanta,Mrinal, AU - Giri,Shiba Shankar, Y1 - 2016/11/30/ PY - 2016/07/22/received PY - 2016/11/14/revised PY - 2016/11/24/accepted PY - 2016/12/4/pubmed PY - 2017/2/7/medline PY - 2016/12/4/entrez KW - Fatty acid KW - Fish oil KW - Growth KW - Linseed oil KW - Silver barb KW - Δ6 fad SP - 1 EP - 12 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 205 N2 - Silver barb (Puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in Asia. This study in silver barb was carried out to evaluate the effects of total or partial substitution of dietary fish oil (FO) with linseed oil (LO) on growth, nutrient utilization, whole-body composition, muscle and liver fatty acid composition. Fish (12.1±0.4g of initial body weight) were fed for 60days with five experimental iso-proteinous, iso-lipidic and iso-caloric diets in which FO (control diet) was replaced by 33.3%, 50%, 66.7% and 100% LO. Final weight, weight gain, percent weight gain, SGR decreased linearly (p<0.001) with increasing LO levels in the diets. Dietary LO substitution levels did not significantly (p>0.05) affect the feed conversion ratio (FCR), protein efficiency ratio (PER) and whole body proximate composition. Furthermore, enhanced level of LO increased α-linolenic acid (ALA; 18:3n3) and linoleic acid (LA; 18:2n6) and decreased eicosapentaenoic acid (EPA; 20:5n3) and docosahexaenoic acid (DHA; 22:6n3) in muscle and liver. To understand the molecular mechanism of long chain-polyunsaturated fatty acid (LC-PUFA) biosynthesis, we cloned and characterized the fatty acyl Δ6 desaturase (Δ6 fad) cDNA and investigated its expression in various organs/tissues following replacement of FO with LO in the diet. The full-length Δ6 fad cDNA was 2056bp encoding 444 amino acids and was widely expressed in various organs/tissues. Replacement of FO with LO increased the expression of Δ6 fad mRNA in liver, muscle and intestine but no significant difference was found in the brain. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/27913275/Dietary_fish_oil_replacement_by_linseed_oil:_Effect_on_growth_nutrient_utilization_tissue_fatty_acid_composition_and_desaturase_gene_expression_in_silver_barb__Puntius_gonionotus__fingerlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(16)30176-2 DB - PRIME DP - Unbound Medicine ER -