DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor.
Biosens Bioelectron. 2017 Apr 15; 90:251-257.BB

Abstract

Proximal metallic nanoparticles (NPs) could quench the electrochemiluminescence (ECL) emission of semiconductor quantum dots (QDs) due to Förster energy transfer (FRET), but at a certain distance, the coupling of light-emission with surface plasmon resonance (SPR) result in enhanced ECL. Thus, the modification strategies and distances control between QDs and metallic NPs are critical for the ECL intensity of QDs. In this strategy, a SPR enhanced ECL sensor based on DNA tetrahedral scaffolds modified platform was reported for the detection of telomerase activity. Due to the rigid three-dimensional structure, DNA tetrahedral scaffolds grafting on the electrode surface could accurately modulate the distance between CdS QDs and luminol labelled gold nanoparticles (L-Au NPs), meanwhile provide an enhanced spatial dimension and accessibility for the assembly of multiple L-Au NPs. The ECL intensities of both CdS QDs (-1.25V vs. SCE) and luminol (+0.33V vs. SCE) gradually increased along with the formation of multiple L-Au NPs at the vertex of DNA tetrahedral scaffolds induced by telomerase, bringing in a dual-potential ECL analysis. The proposed method showed high sensitivity for the identification of telomerase and was successfully applied for the differentiation of cancer cells from normal cells. This work suggests that DNA tetrahedral scaffolds could serve as an excellent choice for the construction of SPR-ECL system.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Feng QM

State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China.

MeSH

Biosensing TechniquesCadmium CompoundsCell Line, TumorGoldHeLa CellsHumansImmobilized Nucleic AcidsLuminescent AgentsLuminescent MeasurementsLuminolMetal NanoparticlesNanostructuresNeoplasmsQuantum DotsSelenium CompoundsSurface Plasmon ResonanceTelomerase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27914369

Citation

Feng, Qiu-Mei, et al. "DNA Tetrahedral Scaffolds-based Platform for the Construction of Electrochemiluminescence Biosensor." Biosensors & Bioelectronics, vol. 90, 2017, pp. 251-257.

Feng QM, Zhou Z, Li MX, et al. DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor. Biosens Bioelectron. 2017;90:251-257.

Feng, Q. M., Zhou, Z., Li, M. X., Zhao, W., Xu, J. J., & Chen, H. Y. (2017). DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor. Biosensors & Bioelectronics, 90, 251-257. https://doi.org/10.1016/j.bios.2016.11.060

Feng QM, et al. DNA Tetrahedral Scaffolds-based Platform for the Construction of Electrochemiluminescence Biosensor. Biosens Bioelectron. 2017 Apr 15;90:251-257. PubMed PMID: 27914369.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor. AU - Feng,Qiu-Mei, AU - Zhou,Zhen, AU - Li,Mei-Xing, AU - Zhao,Wei, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2016/11/27/ PY - 2016/08/09/received PY - 2016/11/14/revised PY - 2016/11/25/accepted PY - 2016/12/4/pubmed PY - 2017/3/14/medline PY - 2016/12/4/entrez KW - Au nanoparticles KW - CdS quantum dots KW - DNA tetrahedral scaffolds KW - Electrochemiluminescence KW - Surface plasmon resonance SP - 251 EP - 257 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 90 N2 - Proximal metallic nanoparticles (NPs) could quench the electrochemiluminescence (ECL) emission of semiconductor quantum dots (QDs) due to Förster energy transfer (FRET), but at a certain distance, the coupling of light-emission with surface plasmon resonance (SPR) result in enhanced ECL. Thus, the modification strategies and distances control between QDs and metallic NPs are critical for the ECL intensity of QDs. In this strategy, a SPR enhanced ECL sensor based on DNA tetrahedral scaffolds modified platform was reported for the detection of telomerase activity. Due to the rigid three-dimensional structure, DNA tetrahedral scaffolds grafting on the electrode surface could accurately modulate the distance between CdS QDs and luminol labelled gold nanoparticles (L-Au NPs), meanwhile provide an enhanced spatial dimension and accessibility for the assembly of multiple L-Au NPs. The ECL intensities of both CdS QDs (-1.25V vs. SCE) and luminol (+0.33V vs. SCE) gradually increased along with the formation of multiple L-Au NPs at the vertex of DNA tetrahedral scaffolds induced by telomerase, bringing in a dual-potential ECL analysis. The proposed method showed high sensitivity for the identification of telomerase and was successfully applied for the differentiation of cancer cells from normal cells. This work suggests that DNA tetrahedral scaffolds could serve as an excellent choice for the construction of SPR-ECL system. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27914369/DNA_tetrahedral_scaffolds_based_platform_for_the_construction_of_electrochemiluminescence_biosensor_ DB - PRIME DP - Unbound Medicine ER -

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DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor.Biosens Bioelectron. 2017 Apr 15; 90:251-257.BB