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Effect of flaxseed oil and microalgae DHA on the production performance, fatty acids and total lipids of egg yolk and plasma in laying hens.

Abstract

The incorporation of omega-3 polyunsaturated fatty acids (PUFA) in the egg is dependent on both the transfer efficiency of preformed dietary omega-3 fatty acids to the eggs as well as endogenous PUFA metabolism and deposition. Employing an experimental design consisting of 70 Lohmann LSL-Classic hens (n=10/treatment) in a 6-week feeding trial, we examined the impact of graded levels of either flaxseed oil (alpha-linolenic acid, ALA) or algal DHA (preformed docosahexaenoic acid, DHA), each supplying 0.20%, 0.40% and 0.60% total omega-3s. The control diet was practically low in omega-3s. Study parameters included monitoring the changes of fatty acid contents in yolk, measures of hen performance, eggshell quality, total lipids and fatty acid contents of plasma. Data were analysed as a complete randomized design using Proc Mixed procedure of SAS. No significant differences were observed between treatments with respect to hen performance, eggshell quality and cholesterol content in plasma and egg yolk. Individual and total omega-3 PUFA in the yolk and plasma increased (P<0.0001) linearly as a function of total omega-3 PUFA intake. At the highest inclusion levels, DHA-fed hens incorporated 3-fold more DHA in eggs compared with ALA-fed hens (179±5.55 vs. 66.7±2.25mg/yolk, respectively). In both treatment groups, maximal enrichment of total n-3 PUFA was observed by week-2, declined by week-4 and leveled thereafter. In addition, accumulation of DHA in egg yolk showed linear (P<0.0001) and quadratic (P<0.05) effects for flaxseed oil (R2=0.89) and algal DHA (R2=0.95). The current data, based on defined level of total omega-3s in the background diet, provides evidence to suggest that exogenous as well as endogenous synthesis of DHA may be subject to a similar basis of regulation, and serve to highlight potential regulatory aspects explaining the limitations in the deposition of endogenously produced omega-3 LCPUFA.

Authors+Show Affiliations

Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.Department of Chemistry, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada; Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada; Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba R3T 2E1, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Research Centre, Winnipeg MB R2H 2A6, Canada. Electronic address: j_house@umanitoba.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27914517

Citation

Neijat, M, et al. "Effect of Flaxseed Oil and Microalgae DHA On the Production Performance, Fatty Acids and Total Lipids of Egg Yolk and Plasma in Laying Hens." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 115, 2016, pp. 77-88.
Neijat M, Ojekudo O, House JD. Effect of flaxseed oil and microalgae DHA on the production performance, fatty acids and total lipids of egg yolk and plasma in laying hens. Prostaglandins Leukot Essent Fatty Acids. 2016;115:77-88.
Neijat, M., Ojekudo, O., & House, J. D. (2016). Effect of flaxseed oil and microalgae DHA on the production performance, fatty acids and total lipids of egg yolk and plasma in laying hens. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 115, 77-88. https://doi.org/10.1016/j.plefa.2016.10.010
Neijat M, Ojekudo O, House JD. Effect of Flaxseed Oil and Microalgae DHA On the Production Performance, Fatty Acids and Total Lipids of Egg Yolk and Plasma in Laying Hens. Prostaglandins Leukot Essent Fatty Acids. 2016;115:77-88. PubMed PMID: 27914517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of flaxseed oil and microalgae DHA on the production performance, fatty acids and total lipids of egg yolk and plasma in laying hens. AU - Neijat,M, AU - Ojekudo,O, AU - House,J D, Y1 - 2016/11/09/ PY - 2016/08/12/received PY - 2016/10/24/revised PY - 2016/10/30/accepted PY - 2016/12/5/entrez PY - 2016/12/5/pubmed PY - 2017/11/14/medline KW - Egg yolk KW - Fatty acid profile KW - Flaxseed oil and algal DHA SP - 77 EP - 88 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 115 N2 - The incorporation of omega-3 polyunsaturated fatty acids (PUFA) in the egg is dependent on both the transfer efficiency of preformed dietary omega-3 fatty acids to the eggs as well as endogenous PUFA metabolism and deposition. Employing an experimental design consisting of 70 Lohmann LSL-Classic hens (n=10/treatment) in a 6-week feeding trial, we examined the impact of graded levels of either flaxseed oil (alpha-linolenic acid, ALA) or algal DHA (preformed docosahexaenoic acid, DHA), each supplying 0.20%, 0.40% and 0.60% total omega-3s. The control diet was practically low in omega-3s. Study parameters included monitoring the changes of fatty acid contents in yolk, measures of hen performance, eggshell quality, total lipids and fatty acid contents of plasma. Data were analysed as a complete randomized design using Proc Mixed procedure of SAS. No significant differences were observed between treatments with respect to hen performance, eggshell quality and cholesterol content in plasma and egg yolk. Individual and total omega-3 PUFA in the yolk and plasma increased (P<0.0001) linearly as a function of total omega-3 PUFA intake. At the highest inclusion levels, DHA-fed hens incorporated 3-fold more DHA in eggs compared with ALA-fed hens (179±5.55 vs. 66.7±2.25mg/yolk, respectively). In both treatment groups, maximal enrichment of total n-3 PUFA was observed by week-2, declined by week-4 and leveled thereafter. In addition, accumulation of DHA in egg yolk showed linear (P<0.0001) and quadratic (P<0.05) effects for flaxseed oil (R2=0.89) and algal DHA (R2=0.95). The current data, based on defined level of total omega-3s in the background diet, provides evidence to suggest that exogenous as well as endogenous synthesis of DHA may be subject to a similar basis of regulation, and serve to highlight potential regulatory aspects explaining the limitations in the deposition of endogenously produced omega-3 LCPUFA. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/27914517/Effect_of_flaxseed_oil_and_microalgae_DHA_on_the_production_performance_fatty_acids_and_total_lipids_of_egg_yolk_and_plasma_in_laying_hens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(16)30124-7 DB - PRIME DP - Unbound Medicine ER -