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Virulence traits and different nle profiles in cattle and human verotoxin-producing Escherichia coli O157:H7 strains from Argentina.
Microb Pathog. 2017 Jan; 102:102-108.MP

Abstract

Verotoxin-producing E. coli (VTEC) O157:H7 is the dominant serotype isolated from patients with HUS and, Argentina has the highest rate of HUS in the world. Molecular typing had allowed to identify subpopulations related to the origin and virulence of O157:H7 strains. Our aim was to perform a genetic characterization of 43 O157:H7 strains isolated in Argentine mostly from cattle and humans in order to establish the potential public health risk. For it, we used a combination of molecular subtyping methods in order to identify clade 8_rhsA (C3468G), LSPA-6 and virulence profiles and, a cytotoxicity assay on Vero cell. All isolates carried the clade 8 SNP variant and 98% of them belonged to lineage I/II (2% lineage II). Isolates were grouped into eleven nle profiles, 46% were positive for all nle genes, while the remaining isolates, except two, showed incomplete OI-71, particularly lacked nleF. All isolates showed the plasmid profile ehxA-espP-katP-stcE and harbored ehaA, elfA, iha and lpfA variants lpfA1-3 and lpfA2-2 and, ECSP_0242. The frequencies of the remaining ECSP genes were 95% ECSP_2687, 88% ECSP_3286, 86% ECSP_3620, 53% ECSP_2870/2872 and 44% ECSP_1733. All O157:H7 strains, except the isolate identified as lineage II, were cytotoxic on Vero cells. Among Argentinean strains, most genetic markers occur at equal relative frequencies among clinical and bovine isolates, showing diversity mostly in nle genes profiles. The belonging of the isolates to hypervirulent clade 8 and lineage I/II, the high prevalence of nle and putative virulence factors genes, would allow assigning most O157:H7 strains of this region a high risk to public health.

Authors+Show Affiliations

Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina.Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina.Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina.Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina. Electronic address: avbustaman@vet.unicen.edu.ar.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27914959

Citation

González, Juliana, et al. "Virulence Traits and Different Nle Profiles in Cattle and Human Verotoxin-producing Escherichia Coli O157:H7 Strains From Argentina." Microbial Pathogenesis, vol. 102, 2017, pp. 102-108.
González J, Sanso AM, Cadona JS, et al. Virulence traits and different nle profiles in cattle and human verotoxin-producing Escherichia coli O157:H7 strains from Argentina. Microb Pathog. 2017;102:102-108.
González, J., Sanso, A. M., Cadona, J. S., & Bustamante, A. V. (2017). Virulence traits and different nle profiles in cattle and human verotoxin-producing Escherichia coli O157:H7 strains from Argentina. Microbial Pathogenesis, 102, 102-108. https://doi.org/10.1016/j.micpath.2016.11.022
González J, et al. Virulence Traits and Different Nle Profiles in Cattle and Human Verotoxin-producing Escherichia Coli O157:H7 Strains From Argentina. Microb Pathog. 2017;102:102-108. PubMed PMID: 27914959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virulence traits and different nle profiles in cattle and human verotoxin-producing Escherichia coli O157:H7 strains from Argentina. AU - González,Juliana, AU - Sanso,Andrea Mariel, AU - Cadona,Jimena Soledad, AU - Bustamante,Ana Victoria, Y1 - 2016/11/30/ PY - 2016/08/02/received PY - 2016/10/28/revised PY - 2016/11/29/accepted PY - 2016/12/5/pubmed PY - 2017/4/7/medline PY - 2016/12/5/entrez KW - O157:H7 KW - Subtyping KW - Verotoxin-producing Escherichia coli KW - Virulence factors SP - 102 EP - 108 JF - Microbial pathogenesis JO - Microb Pathog VL - 102 N2 - Verotoxin-producing E. coli (VTEC) O157:H7 is the dominant serotype isolated from patients with HUS and, Argentina has the highest rate of HUS in the world. Molecular typing had allowed to identify subpopulations related to the origin and virulence of O157:H7 strains. Our aim was to perform a genetic characterization of 43 O157:H7 strains isolated in Argentine mostly from cattle and humans in order to establish the potential public health risk. For it, we used a combination of molecular subtyping methods in order to identify clade 8_rhsA (C3468G), LSPA-6 and virulence profiles and, a cytotoxicity assay on Vero cell. All isolates carried the clade 8 SNP variant and 98% of them belonged to lineage I/II (2% lineage II). Isolates were grouped into eleven nle profiles, 46% were positive for all nle genes, while the remaining isolates, except two, showed incomplete OI-71, particularly lacked nleF. All isolates showed the plasmid profile ehxA-espP-katP-stcE and harbored ehaA, elfA, iha and lpfA variants lpfA1-3 and lpfA2-2 and, ECSP_0242. The frequencies of the remaining ECSP genes were 95% ECSP_2687, 88% ECSP_3286, 86% ECSP_3620, 53% ECSP_2870/2872 and 44% ECSP_1733. All O157:H7 strains, except the isolate identified as lineage II, were cytotoxic on Vero cells. Among Argentinean strains, most genetic markers occur at equal relative frequencies among clinical and bovine isolates, showing diversity mostly in nle genes profiles. The belonging of the isolates to hypervirulent clade 8 and lineage I/II, the high prevalence of nle and putative virulence factors genes, would allow assigning most O157:H7 strains of this region a high risk to public health. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/27914959/Virulence_traits_and_different_nle_profiles_in_cattle_and_human_verotoxin_producing_Escherichia_coli_O157:H7_strains_from_Argentina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(16)30430-2 DB - PRIME DP - Unbound Medicine ER -