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Antimicrobial resistance and molecular subtypes of Salmonella enterica serovar Typhi isolates from Kolkata, India over a 15 years period 1998-2012.
Int J Med Microbiol. 2017 Jan; 307(1):28-36.IJ

Abstract

Typhoid fever, caused by Salmonella enterica serovar Typhi (S. Typhi), remains an unresolved public health problem in India. Emergence of antimicrobial resistant strains poses a great concern for typhoid treatment and influences reshaping of current S. Typhi population. We included representative S. Typhi strains (n=164) from retrospective studies, both community and hospital based, conducted at National Institute of Cholera and Enteric Diseases, Kolkata during 15 years period (1998-2012) to analyze their antimicrobial resistance (AMR) profiles, mechanism of AMR and molecular subtypes of the strains. More than 60% of the S. Typhi isolates were obtained from community based studies. During the study period, steady decline (46.4%-15.6%) in isolation of multidrug-resistant (MDR, resistant to ampicillin, chloramphenicol and co-trimoxazole) S. Typhi was noticed with parallel increase of nalidixic acid-resistant (NALR) strains (60.7%-93.8%) and ciprofloxacin resistant (CIPR) strains (0%-25%). Of 53 MDR strains, 46 (86.8%) were NALR showing decreased ciprofloxacin susceptible (DCS) (MIC for ciprofloxacin 0.12-0.5μg/ml) phenotype. Conjugative IncHI1 (230kb) and non-conjugative non-IncHI1 (180kb) plasmids were found in 23 (43.4%) and 14 (26.4%) MDR strains respectively, plasmid was absent in 16 (30.2%) MDR strains. MDR strains with or without plasmid shared the same set of resistance genes (blaTEM-1, catA1, sul1, sul2, strA and strB) and class 1 integron possessing dfrA7 gene cassette. Two S. Typhi strains harbored 50kb transferrable plasmids carrying dfrA15 and aadA1 gene cassettes in class 1 integron. The majority of the strains (135/164, 82.3%) belonged to H58 haplotype. Among the MDR isolates, fluoroquinolone resistant or combined resistant isolates (n=147), 127 (86.4%) were H58 and 20 (13.6%) belonged to non-H58. NALRS. Typhi strains with decreased susceptibility or resistance to ciprofloxacin had point mutation(s) in quinolone resistance-determining region of gyrA and parC genes. Pulsed-field gel electrophoresis showed more diversity among NALRS. Typhi than MDR strains. Results of this study generated information useful for better understanding of the disease epidemiology and its control in endemic settings.

Authors+Show Affiliations

Microbiology Division, National Institute of Cholera and Enteric Diseases, P-33 C.I.T Road, Kolkata, West Bengal 700010, India.Microbiology Division, National Institute of Cholera and Enteric Diseases, P-33 C.I.T Road, Kolkata, West Bengal 700010, India.Microbiology Division, Apollo Gleneagles Hospitals, 58 Canal Circular Road, Kolkata, West Bengal 700054, India.Microbiology Division, Calcutta Medical Research Institute, 7/2 Diamond Harbour Road, Kolkata, West Bengal 700027, India.Microbiology Division, National Institute of Cholera and Enteric Diseases, P-33 C.I.T Road, Kolkata, West Bengal 700010, India. Electronic address: shanta1232001@yahoo.co.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27916384

Citation

Das, Surojit, et al. "Antimicrobial Resistance and Molecular Subtypes of Salmonella Enterica Serovar Typhi Isolates From Kolkata, India Over a 15 Years Period 1998-2012." International Journal of Medical Microbiology : IJMM, vol. 307, no. 1, 2017, pp. 28-36.
Das S, Samajpati S, Ray U, et al. Antimicrobial resistance and molecular subtypes of Salmonella enterica serovar Typhi isolates from Kolkata, India over a 15 years period 1998-2012. Int J Med Microbiol. 2017;307(1):28-36.
Das, S., Samajpati, S., Ray, U., Roy, I., & Dutta, S. (2017). Antimicrobial resistance and molecular subtypes of Salmonella enterica serovar Typhi isolates from Kolkata, India over a 15 years period 1998-2012. International Journal of Medical Microbiology : IJMM, 307(1), 28-36. https://doi.org/10.1016/j.ijmm.2016.11.006
Das S, et al. Antimicrobial Resistance and Molecular Subtypes of Salmonella Enterica Serovar Typhi Isolates From Kolkata, India Over a 15 Years Period 1998-2012. Int J Med Microbiol. 2017;307(1):28-36. PubMed PMID: 27916384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial resistance and molecular subtypes of Salmonella enterica serovar Typhi isolates from Kolkata, India over a 15 years period 1998-2012. AU - Das,Surojit, AU - Samajpati,Sriparna, AU - Ray,Ujjwayini, AU - Roy,Indranil, AU - Dutta,Shanta, Y1 - 2016/11/25/ PY - 2016/08/29/received PY - 2016/11/19/revised PY - 2016/11/20/accepted PY - 2016/12/6/pubmed PY - 2017/3/9/medline PY - 2016/12/6/entrez KW - Antimicrobial resistance KW - H58 haplotype KW - IncHI1 plasmid KW - Pulsed-field gel electrophoresis KW - Quinoloneresistance-determining region KW - Salmonella Typhi SP - 28 EP - 36 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 307 IS - 1 N2 - Typhoid fever, caused by Salmonella enterica serovar Typhi (S. Typhi), remains an unresolved public health problem in India. Emergence of antimicrobial resistant strains poses a great concern for typhoid treatment and influences reshaping of current S. Typhi population. We included representative S. Typhi strains (n=164) from retrospective studies, both community and hospital based, conducted at National Institute of Cholera and Enteric Diseases, Kolkata during 15 years period (1998-2012) to analyze their antimicrobial resistance (AMR) profiles, mechanism of AMR and molecular subtypes of the strains. More than 60% of the S. Typhi isolates were obtained from community based studies. During the study period, steady decline (46.4%-15.6%) in isolation of multidrug-resistant (MDR, resistant to ampicillin, chloramphenicol and co-trimoxazole) S. Typhi was noticed with parallel increase of nalidixic acid-resistant (NALR) strains (60.7%-93.8%) and ciprofloxacin resistant (CIPR) strains (0%-25%). Of 53 MDR strains, 46 (86.8%) were NALR showing decreased ciprofloxacin susceptible (DCS) (MIC for ciprofloxacin 0.12-0.5μg/ml) phenotype. Conjugative IncHI1 (230kb) and non-conjugative non-IncHI1 (180kb) plasmids were found in 23 (43.4%) and 14 (26.4%) MDR strains respectively, plasmid was absent in 16 (30.2%) MDR strains. MDR strains with or without plasmid shared the same set of resistance genes (blaTEM-1, catA1, sul1, sul2, strA and strB) and class 1 integron possessing dfrA7 gene cassette. Two S. Typhi strains harbored 50kb transferrable plasmids carrying dfrA15 and aadA1 gene cassettes in class 1 integron. The majority of the strains (135/164, 82.3%) belonged to H58 haplotype. Among the MDR isolates, fluoroquinolone resistant or combined resistant isolates (n=147), 127 (86.4%) were H58 and 20 (13.6%) belonged to non-H58. NALRS. Typhi strains with decreased susceptibility or resistance to ciprofloxacin had point mutation(s) in quinolone resistance-determining region of gyrA and parC genes. Pulsed-field gel electrophoresis showed more diversity among NALRS. Typhi than MDR strains. Results of this study generated information useful for better understanding of the disease epidemiology and its control in endemic settings. SN - 1618-0607 UR - https://www.unboundmedicine.com/medline/citation/27916384/Antimicrobial_resistance_and_molecular_subtypes_of_Salmonella_enterica_serovar_Typhi_isolates_from_Kolkata_India_over_a_15_years_period_1998_2012_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(16)30235-1 DB - PRIME DP - Unbound Medicine ER -