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[Evaluation of an Xpert EV (Cepheid®) molecular diagnostic technique for enteroviral meningitis].
An Pediatr (Barc). 2017 Oct; 87(4):201-205.AP

Abstract

INTRODUCTION

Polymerase chain reaction (PCR) assays have shown to be useful and quick for the diagnosis of enterovirus in aseptic meningitis. The aim of our study was to analyse the changes in clinical practice after the introduction of a real-time polymerase chain reaction (RT-PCR) technique using the Xpert EV (Cepheid®) assay for the qualitative detection of enterovirus RNA in cerebrospinal fluid specimens from children with suspected viral meningitis.

METHODS

A retrospective study was performed in children older than 1year, diagnosed with enterovirus meningitis in a third level hospital from November 2006 to February 2013. The first period, before the availability of Xpert EV (Cepheid®) (Group1, November 2006-August 2010) was compared with the later period (Group2, September 2010-February 2013). Clinical characteristics, the mean length of stay, and the cost per inpatient cases, were compared between the 2periods.

RESULTS

Forty-one patients (60.9% male) were included, with a median age of 64 months (interquartile range 28-96). Twenty-six patients (63.4%) were included in Group2. There were non-statistically significant differences in the epidemiological, disease severity, and laboratory characteristics between both periods of study. A significant difference was observed in the mean length of stay, with it being shorter in Group2 (48hours vs 40.5hours, P=.039), and a significant lower inpatient cost per case (€779.77 vs €656.05, P<.05).

CONCLUSION

Xpert EV (Cepheid®) assay was useful for decreasing the length of hospital stay and the costs associated with hospitalisation in children with enterovirus meningitis.

Authors+Show Affiliations

Servicio de Pediatría, Hospital Universitario de Getafe, Getafe, Madrid, España. Electronic address: natalia.alonso.perez@gmail.com.Servicio de Pediatría, Hospital Universitario de Getafe, Getafe, Madrid, España.Servicio de Pediatría, Hospital Universitario de Getafe, Getafe, Madrid, España.Servicio de Pediatría, Hospital Universitario de Getafe, Getafe, Madrid, España; Hospital Universitario de Getafe, Getafe, Madrid, España.Servicio de Microbiología, Hospital Universitario de Getafe, Getafe, Madrid, España.Servicio de Microbiología, Hospital Universitario de Getafe, Getafe, Madrid, España.Servicio de Pediatría, Hospital Universitario de Getafe, Getafe, Madrid, España.

Pub Type(s)

Journal Article

Language

spa

PubMed ID

27919639

Citation

Alonso Pérez, Natalia, et al. "[Evaluation of an Xpert EV (Cepheid®) Molecular Diagnostic Technique for Enteroviral Meningitis]." Anales De Pediatria (Barcelona, Spain : 2003), vol. 87, no. 4, 2017, pp. 201-205.
Alonso Pérez N, Sagastizabal Cardelus B, Prieto Tato LM, et al. [Evaluation of an Xpert EV (Cepheid®) molecular diagnostic technique for enteroviral meningitis]. An Pediatr (Barc). 2017;87(4):201-205.
Alonso Pérez, N., Sagastizabal Cardelus, B., Prieto Tato, L. M., Guillén Martín, S., González Torralba, A., García Bermejo, I., & Ramos Amador, J. T. (2017). [Evaluation of an Xpert EV (Cepheid®) molecular diagnostic technique for enteroviral meningitis]. Anales De Pediatria (Barcelona, Spain : 2003), 87(4), 201-205. https://doi.org/10.1016/j.anpedi.2016.09.010
Alonso Pérez N, et al. [Evaluation of an Xpert EV (Cepheid®) Molecular Diagnostic Technique for Enteroviral Meningitis]. An Pediatr (Barc). 2017;87(4):201-205. PubMed PMID: 27919639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Evaluation of an Xpert EV (Cepheid®) molecular diagnostic technique for enteroviral meningitis]. AU - Alonso Pérez,Natalia, AU - Sagastizabal Cardelus,Belén, AU - Prieto Tato,Luis Manuel, AU - Guillén Martín,Sara, AU - González Torralba,Ana, AU - García Bermejo,Isabel, AU - Ramos Amador,José Tomás, Y1 - 2016/12/03/ PY - 2016/05/25/received PY - 2016/09/19/revised PY - 2016/09/26/accepted PY - 2016/12/7/pubmed PY - 2019/1/25/medline PY - 2016/12/7/entrez KW - Aseptic meningitis KW - Enterovirus KW - Meningitis aséptica KW - Polymerase chain reaction KW - Reacción en cadena de la polimerasa SP - 201 EP - 205 JF - Anales de pediatria (Barcelona, Spain : 2003) JO - An Pediatr (Barc) VL - 87 IS - 4 N2 - INTRODUCTION: Polymerase chain reaction (PCR) assays have shown to be useful and quick for the diagnosis of enterovirus in aseptic meningitis. The aim of our study was to analyse the changes in clinical practice after the introduction of a real-time polymerase chain reaction (RT-PCR) technique using the Xpert EV (Cepheid®) assay for the qualitative detection of enterovirus RNA in cerebrospinal fluid specimens from children with suspected viral meningitis. METHODS: A retrospective study was performed in children older than 1year, diagnosed with enterovirus meningitis in a third level hospital from November 2006 to February 2013. The first period, before the availability of Xpert EV (Cepheid®) (Group1, November 2006-August 2010) was compared with the later period (Group2, September 2010-February 2013). Clinical characteristics, the mean length of stay, and the cost per inpatient cases, were compared between the 2periods. RESULTS: Forty-one patients (60.9% male) were included, with a median age of 64 months (interquartile range 28-96). Twenty-six patients (63.4%) were included in Group2. There were non-statistically significant differences in the epidemiological, disease severity, and laboratory characteristics between both periods of study. A significant difference was observed in the mean length of stay, with it being shorter in Group2 (48hours vs 40.5hours, P=.039), and a significant lower inpatient cost per case (€779.77 vs €656.05, P<.05). CONCLUSION: Xpert EV (Cepheid®) assay was useful for decreasing the length of hospital stay and the costs associated with hospitalisation in children with enterovirus meningitis. SN - 1695-9531 UR - https://www.unboundmedicine.com/medline/citation/27919639/[Evaluation_of_an_Xpert_EV__Cepheid®__molecular_diagnostic_technique_for_enteroviral_meningitis]_ L2 - http://www.elsevier.es/en/linksolver/ft/pii/S1695-4033(16)30270-3 DB - PRIME DP - Unbound Medicine ER -