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Metabolic differences between cold stored and machine perfused porcine kidneys: A 1H NMR based study.
Cryobiology. 2017 02; 74:115-120.C

Abstract

Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two hours following retrieval. Paired kidneys then underwent 24 h of HMP or SCS or served as time zero controls. Metabolite quantification in both storage fluid and kidney tissue was performed using one dimensional 1H NMR spectroscopy. For each metabolite, the net gain for each storage modality was determined by comparing the total amount in each closed system (i.e. total amount in storage fluid and kidney combined) compared with controls. 26 metabolites were included for analysis. Total system metabolite quantities following HMP or SCS were greater for 14 compared with controls (all p < 0.05). In addition to metabolic differences with control kidneys, the net metabolic gain during HMP was greater than SCS for 8 metabolites (all p < 0.05). These included metabolites related to central metabolism (lactate, glutamate, aspartate, fumarate and acetate). The metabolic environments of both perfusion fluid and the kidney tissue are strikingly different between SCS and HMP systems in this animal model. The total amount of central metabolites such as lactate and glutamate observed in the HMP kidney system suggests a greater degree of de novo metabolic activity than in the SCS system. Maintenance of central metabolic pathways may contribute to the clinical benefits of HMP.

Authors+Show Affiliations

Department of Renal Surgery, University Hospitals Birmingham, Birmingham, UK; Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Department of Renal Surgery, University Hospitals Birmingham, Birmingham, UK; Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.Department of Renal Surgery, University Hospitals Birmingham, Birmingham, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27919740

Citation

Nath, Jay, et al. "Metabolic Differences Between Cold Stored and Machine Perfused Porcine Kidneys: a 1H NMR Based Study." Cryobiology, vol. 74, 2017, pp. 115-120.
Nath J, Smith TB, Patel K, et al. Metabolic differences between cold stored and machine perfused porcine kidneys: A 1H NMR based study. Cryobiology. 2017;74:115-120.
Nath, J., Smith, T. B., Patel, K., Ebbs, S. R., Hollis, A., Tennant, D. A., Ludwig, C., & Ready, A. R. (2017). Metabolic differences between cold stored and machine perfused porcine kidneys: A 1H NMR based study. Cryobiology, 74, 115-120. https://doi.org/10.1016/j.cryobiol.2016.11.006
Nath J, et al. Metabolic Differences Between Cold Stored and Machine Perfused Porcine Kidneys: a 1H NMR Based Study. Cryobiology. 2017;74:115-120. PubMed PMID: 27919740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic differences between cold stored and machine perfused porcine kidneys: A 1H NMR based study. AU - Nath,Jay, AU - Smith,Tom B, AU - Patel,Kamlesh, AU - Ebbs,Sam R, AU - Hollis,Alex, AU - Tennant,Daniel A, AU - Ludwig,Christian, AU - Ready,Andrew R, Y1 - 2016/12/03/ PY - 2016/07/11/received PY - 2016/10/18/revised PY - 2016/11/21/accepted PY - 2016/12/7/pubmed PY - 2017/9/8/medline PY - 2016/12/7/entrez KW - Hypothermic machine perfusion KW - Kidney KW - Metabolism KW - NMR KW - Organ preservation KW - Transplantation SP - 115 EP - 120 JF - Cryobiology JO - Cryobiology VL - 74 N2 - Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two hours following retrieval. Paired kidneys then underwent 24 h of HMP or SCS or served as time zero controls. Metabolite quantification in both storage fluid and kidney tissue was performed using one dimensional 1H NMR spectroscopy. For each metabolite, the net gain for each storage modality was determined by comparing the total amount in each closed system (i.e. total amount in storage fluid and kidney combined) compared with controls. 26 metabolites were included for analysis. Total system metabolite quantities following HMP or SCS were greater for 14 compared with controls (all p < 0.05). In addition to metabolic differences with control kidneys, the net metabolic gain during HMP was greater than SCS for 8 metabolites (all p < 0.05). These included metabolites related to central metabolism (lactate, glutamate, aspartate, fumarate and acetate). The metabolic environments of both perfusion fluid and the kidney tissue are strikingly different between SCS and HMP systems in this animal model. The total amount of central metabolites such as lactate and glutamate observed in the HMP kidney system suggests a greater degree of de novo metabolic activity than in the SCS system. Maintenance of central metabolic pathways may contribute to the clinical benefits of HMP. SN - 1090-2392 UR - https://www.unboundmedicine.com/medline/citation/27919740/Metabolic_differences_between_cold_stored_and_machine_perfused_porcine_kidneys:_A_1H_NMR_based_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0011-2240(16)30439-4 DB - PRIME DP - Unbound Medicine ER -