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Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPARα-Dependent AMPK Activation.
J Cell Physiol. 2017 Aug; 232(8):2168-2177.JC

Abstract

BACKGROUND

Palmitoleic acid, since described as lipokine, increases glucose uptake by modulation of 5'AMP-activated protein kinase (AMPK), as well as increasing lipolysis by activation of peroxisome proliferator-activated receptor-α (PPARα), in adipose tissue. However, in liver, the effects of palmitoleic acid on glucose metabolism and the role of PPARα remain unknown.

OBJECTIVE

To investigate whether palmitoleic acid improved the hepatic insulin sensitivity of obese mice.

METHODS

C57BL6 and PPARα knockout (KO) mice were fed for 12 weeks with a standard diet (SD) or high-fat diet (HF), and in the last 2 weeks were treated with oleic or palmitoleic acid.

RESULTS

Palmitoleic acid promoted a faster uptake of glucose in the body, associated with higher insulin concentration; however, even when stimulated with insulin, palmitoleic acid did not modulate the insulin pathway (AKT, IRS). Palmitoleic acid increased the phosphorylation of AMPK, upregulated glucokinase and downregulated SREBP-1. Regarding AMPK downstream, palmitoleic acid increased the production of FGF-21 and stimulated the expression of PPARα. Palmitoleic acid treatment did not increase AMPK phosphorylation, modulate glucokinase or increase FGF-21 in liver of PPARα KO mice.

CONCLUSIONS

In mice fed with a high-fat diet, palmitoleic acid supplementation stimulated the uptake of glucose in liver through activation of AMPK and FGF-21, dependent on PPARα. J. Cell. Physiol. 232: 2168-2177, 2017. © 2016 Wiley Periodicals, Inc.

Authors+Show Affiliations

Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27925195

Citation

de Souza, Camila O., et al. "Palmitoleic Acid Improves Metabolic Functions in Fatty Liver By PPARα-Dependent AMPK Activation." Journal of Cellular Physiology, vol. 232, no. 8, 2017, pp. 2168-2177.
de Souza CO, Teixeira AAS, Biondo LA, et al. Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPARα-Dependent AMPK Activation. J Cell Physiol. 2017;232(8):2168-2177.
de Souza, C. O., Teixeira, A. A. S., Biondo, L. A., Lima Junior, E. A., Batatinha, H. A. P., & Rosa Neto, J. C. (2017). Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPARα-Dependent AMPK Activation. Journal of Cellular Physiology, 232(8), 2168-2177. https://doi.org/10.1002/jcp.25715
de Souza CO, et al. Palmitoleic Acid Improves Metabolic Functions in Fatty Liver By PPARα-Dependent AMPK Activation. J Cell Physiol. 2017;232(8):2168-2177. PubMed PMID: 27925195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPARα-Dependent AMPK Activation. AU - de Souza,Camila O, AU - Teixeira,Alexandre A S, AU - Biondo,Luana A, AU - Lima Junior,Edson A, AU - Batatinha,Helena A P, AU - Rosa Neto,Jose C, Y1 - 2017/03/24/ PY - 2016/10/27/received PY - 2016/11/30/accepted PY - 2016/12/8/pubmed PY - 2017/8/8/medline PY - 2016/12/8/entrez SP - 2168 EP - 2177 JF - Journal of cellular physiology JO - J Cell Physiol VL - 232 IS - 8 N2 - BACKGROUND: Palmitoleic acid, since described as lipokine, increases glucose uptake by modulation of 5'AMP-activated protein kinase (AMPK), as well as increasing lipolysis by activation of peroxisome proliferator-activated receptor-α (PPARα), in adipose tissue. However, in liver, the effects of palmitoleic acid on glucose metabolism and the role of PPARα remain unknown. OBJECTIVE: To investigate whether palmitoleic acid improved the hepatic insulin sensitivity of obese mice. METHODS: C57BL6 and PPARα knockout (KO) mice were fed for 12 weeks with a standard diet (SD) or high-fat diet (HF), and in the last 2 weeks were treated with oleic or palmitoleic acid. RESULTS: Palmitoleic acid promoted a faster uptake of glucose in the body, associated with higher insulin concentration; however, even when stimulated with insulin, palmitoleic acid did not modulate the insulin pathway (AKT, IRS). Palmitoleic acid increased the phosphorylation of AMPK, upregulated glucokinase and downregulated SREBP-1. Regarding AMPK downstream, palmitoleic acid increased the production of FGF-21 and stimulated the expression of PPARα. Palmitoleic acid treatment did not increase AMPK phosphorylation, modulate glucokinase or increase FGF-21 in liver of PPARα KO mice. CONCLUSIONS: In mice fed with a high-fat diet, palmitoleic acid supplementation stimulated the uptake of glucose in liver through activation of AMPK and FGF-21, dependent on PPARα. J. Cell. Physiol. 232: 2168-2177, 2017. © 2016 Wiley Periodicals, Inc. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/27925195/Palmitoleic_Acid_Improves_Metabolic_Functions_in_Fatty_Liver_by_PPARα_Dependent_AMPK_Activation_ L2 - https://doi.org/10.1002/jcp.25715 DB - PRIME DP - Unbound Medicine ER -