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Immunoadjuvant potential of cross-linked dextran microspheres mixed with chitosan nanospheres encapsulated with tetanus toxoid.
Pharm Biol. 2017 Dec; 55(1):212-217.PB

Abstract

CONTEXT

Nasal mucosa is a desirable route for mucosal vaccine delivery. Mucosal co-administration of chitosan nanoparticles with absorption enhancers such as cross-linked dextran microspheres (CDM, Sephadex®) is a promising antigen delivery system.

OBJECTIVE

In the current study, the chitosan nanospheres loaded with tetanus toxoid (CHT:TT NPs) was prepared and characterized. The immune responses against tetanus toxoid after nasal administration of CHT:TT NPs alone or mixed with CDM were also determined.

MATERIALS AND METHODS

Chitosan nanospheres were prepared by ionic gelation method. Particle size, releasing profile and antigen stability were evaluated by dynamic light scattering, diffusion chamber and SDS-PAGE methods, respectively. Rabbits were nasally immunized with different formulations loaded with 40 Lf TT. After three times immunizations with 2 weeks intervals, sera IgG titres and nasal lavage sIgA titres were determined.

RESULTS

Mean size of CHT NPs and CHT:TT NPs were 205 ± 42 nm and 432 ± 85 nm, respectively. The release profile showed that 42.4 ± 10.5% of TT was released after 30 min and reached to a steady state after 1.5 h. Stability of encapsulated TT in nanospheres was confirmed by SDS-PAGE. The antibody titres showed that CHT:TT NPs-induced antibody titres were higher than TT solution. CHT NPs mixed with CDM induced the systemic IgG and nasal lavage sIgA titres higher than intranasal administration of TT solution (p < 0.001).

DISCUSSION AND CONCLUSION

As the results indicated, these CHT:TT NPs when co-administered with CDM were able to induce more immune responses and have the potential to be used in mucosal immunization.

Authors+Show Affiliations

a Student Research Committee, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.b Department of Pharmaceutical Control, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.c Nanotechnology Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27927058

Citation

Pirouzmand, Haniyeh, et al. "Immunoadjuvant Potential of Cross-linked Dextran Microspheres Mixed With Chitosan Nanospheres Encapsulated With Tetanus Toxoid." Pharmaceutical Biology, vol. 55, no. 1, 2017, pp. 212-217.
Pirouzmand H, Khameneh B, Tafaghodi M. Immunoadjuvant potential of cross-linked dextran microspheres mixed with chitosan nanospheres encapsulated with tetanus toxoid. Pharm Biol. 2017;55(1):212-217.
Pirouzmand, H., Khameneh, B., & Tafaghodi, M. (2017). Immunoadjuvant potential of cross-linked dextran microspheres mixed with chitosan nanospheres encapsulated with tetanus toxoid. Pharmaceutical Biology, 55(1), 212-217.
Pirouzmand H, Khameneh B, Tafaghodi M. Immunoadjuvant Potential of Cross-linked Dextran Microspheres Mixed With Chitosan Nanospheres Encapsulated With Tetanus Toxoid. Pharm Biol. 2017;55(1):212-217. PubMed PMID: 27927058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoadjuvant potential of cross-linked dextran microspheres mixed with chitosan nanospheres encapsulated with tetanus toxoid. AU - Pirouzmand,Haniyeh, AU - Khameneh,Bahman, AU - Tafaghodi,Mohsen, PY - 2016/12/9/entrez PY - 2016/12/9/pubmed PY - 2017/3/11/medline KW - Nasal immunization KW - drug delivery KW - dry powder formulation KW - immune responses SP - 212 EP - 217 JF - Pharmaceutical biology JO - Pharm Biol VL - 55 IS - 1 N2 - CONTEXT: Nasal mucosa is a desirable route for mucosal vaccine delivery. Mucosal co-administration of chitosan nanoparticles with absorption enhancers such as cross-linked dextran microspheres (CDM, Sephadex®) is a promising antigen delivery system. OBJECTIVE: In the current study, the chitosan nanospheres loaded with tetanus toxoid (CHT:TT NPs) was prepared and characterized. The immune responses against tetanus toxoid after nasal administration of CHT:TT NPs alone or mixed with CDM were also determined. MATERIALS AND METHODS: Chitosan nanospheres were prepared by ionic gelation method. Particle size, releasing profile and antigen stability were evaluated by dynamic light scattering, diffusion chamber and SDS-PAGE methods, respectively. Rabbits were nasally immunized with different formulations loaded with 40 Lf TT. After three times immunizations with 2 weeks intervals, sera IgG titres and nasal lavage sIgA titres were determined. RESULTS: Mean size of CHT NPs and CHT:TT NPs were 205 ± 42 nm and 432 ± 85 nm, respectively. The release profile showed that 42.4 ± 10.5% of TT was released after 30 min and reached to a steady state after 1.5 h. Stability of encapsulated TT in nanospheres was confirmed by SDS-PAGE. The antibody titres showed that CHT:TT NPs-induced antibody titres were higher than TT solution. CHT NPs mixed with CDM induced the systemic IgG and nasal lavage sIgA titres higher than intranasal administration of TT solution (p < 0.001). DISCUSSION AND CONCLUSION: As the results indicated, these CHT:TT NPs when co-administered with CDM were able to induce more immune responses and have the potential to be used in mucosal immunization. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/27927058/Immunoadjuvant_potential_of_cross_linked_dextran_microspheres_mixed_with_chitosan_nanospheres_encapsulated_with_tetanus_toxoid_ L2 - http://www.tandfonline.com/doi/full/10.1080/13880209.2016.1257032 DB - PRIME DP - Unbound Medicine ER -