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Production of endogenous hydrogen sulfide in human gingival tissue.
Arch Oral Biol. 2017 Feb; 74:108-113.AO

Abstract

OBJECTIVE

Endogenous hydrogen sulfide (H2S) has recently been shown to play an important role in inflammation, but the role of endogenous H2S in the human gingival tissue is unknown. The aim of this study was to investigate whether gingiva had enzymes for H2S synthesis, and whether the effect of these enzymes for H2S production changed with periodontal inflammation.

DESIGN

Gingival tissues were collected from patients undergoing periodontal operation including gingivitis, moderate chronic periodontitis, severe chronic periodontitis and normal controls. RT-PCR and western blotting were performed to measure mRNA and protein levels of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) for H2S production. Immunohistochemistry was carried out to detect the location of the enzymes. H2S levels and synthesis in gingival tissue were evaluated with modified methylene blue method.

RESULTS

The mRNA and protein of CBS and CSE were both expressed in human gingiva and raised significantly in moderate and severe periodontitis compared of that in healthy control. CBS, but not CSE, increased in gingivitis (p<0.05). However, there was no significant difference of H2S level and synthesis among these groups (p>0.05).

CONCLUSIONS

Both CBS and CSE were expressed in human gingival tissue. The mRNA and protein levels of CBS and CSE were up-regulated in periodontitis.

Authors+Show Affiliations

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.Atherosclerosis Research Center, School of Pharmacy, Department of Pathophysiology, Nanjing Medical University, Nanjing, China.Atherosclerosis Research Center, School of Pharmacy, Department of Pathophysiology, Nanjing Medical University, Nanjing, China.Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China. Electronic address: njcn@njmu.edu.cn.Atherosclerosis Research Center, School of Pharmacy, Department of Pathophysiology, Nanjing Medical University, Nanjing, China. Electronic address: yongji@njmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27930932

Citation

Chun-Mei, Jiang, et al. "Production of Endogenous Hydrogen Sulfide in Human Gingival Tissue." Archives of Oral Biology, vol. 74, 2017, pp. 108-113.
Chun-Mei J, Wu C, Guo-Liang M, et al. Production of endogenous hydrogen sulfide in human gingival tissue. Arch Oral Biol. 2017;74:108-113.
Chun-Mei, J., Wu, C., Guo-Liang, M., Yue, G., Ning, C., & Ji, Y. (2017). Production of endogenous hydrogen sulfide in human gingival tissue. Archives of Oral Biology, 74, 108-113. https://doi.org/10.1016/j.archoralbio.2016.11.016
Chun-Mei J, et al. Production of Endogenous Hydrogen Sulfide in Human Gingival Tissue. Arch Oral Biol. 2017;74:108-113. PubMed PMID: 27930932.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of endogenous hydrogen sulfide in human gingival tissue. AU - Chun-Mei,Jiang, AU - Wu,Chen, AU - Guo-Liang,Meng, AU - Yue,Gu, AU - Ning,Chen, AU - Ji,Yong, Y1 - 2016/11/28/ PY - 2016/06/22/received PY - 2016/10/28/revised PY - 2016/11/27/accepted PY - 2016/12/9/pubmed PY - 2017/12/30/medline PY - 2016/12/9/entrez KW - Cystathionine β-synthase KW - Cystathionine γ-lyase KW - Endogenous hydrogen sulfide KW - Periodontal inflammation SP - 108 EP - 113 JF - Archives of oral biology JO - Arch Oral Biol VL - 74 N2 - OBJECTIVE: Endogenous hydrogen sulfide (H2S) has recently been shown to play an important role in inflammation, but the role of endogenous H2S in the human gingival tissue is unknown. The aim of this study was to investigate whether gingiva had enzymes for H2S synthesis, and whether the effect of these enzymes for H2S production changed with periodontal inflammation. DESIGN: Gingival tissues were collected from patients undergoing periodontal operation including gingivitis, moderate chronic periodontitis, severe chronic periodontitis and normal controls. RT-PCR and western blotting were performed to measure mRNA and protein levels of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) for H2S production. Immunohistochemistry was carried out to detect the location of the enzymes. H2S levels and synthesis in gingival tissue were evaluated with modified methylene blue method. RESULTS: The mRNA and protein of CBS and CSE were both expressed in human gingiva and raised significantly in moderate and severe periodontitis compared of that in healthy control. CBS, but not CSE, increased in gingivitis (p<0.05). However, there was no significant difference of H2S level and synthesis among these groups (p>0.05). CONCLUSIONS: Both CBS and CSE were expressed in human gingival tissue. The mRNA and protein levels of CBS and CSE were up-regulated in periodontitis. SN - 1879-1506 UR - https://www.unboundmedicine.com/medline/citation/27930932/Production_of_endogenous_hydrogen_sulfide_in_human_gingival_tissue_ DB - PRIME DP - Unbound Medicine ER -