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Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia.
Infect Genet Evol. 2017 03; 48:10-18.IG

Abstract

South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n=55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii.

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Authors+Show Affiliations

Lacroix A
Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
Duong V
Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
Hul V
Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
San S
National Veterinary Research Institute, Ministry of Agriculture Forestry and Fisheries, Cambodia.
Davun H
National Veterinary Research Institute, Ministry of Agriculture Forestry and Fisheries, Cambodia.
Omaliss K
Forest Administration, Ministry of Agriculture Forestry and Fisheries, Cambodia.
Chea S
Wildlife Conservation Society, Cambodia.
Hassanin A
Muséum national d'Histoire naturelle, Institut de Systématique, Evolution, Biodiversité (ISYEB), UMR 7205 MNHN CNRS UPMC, France.
Theppangna W
National Animal Health Laboratory, Ministry of Agriculture Forestry and Fisheries, Lao Democratic People's Republic.
Silithammavong S
Wildlife Conservation Society, Lao Democratic People's Republic; Metabiota Inc., Vientiane, Lao Democratic People's Republic.
Khammavong K
Wildlife Conservation Society, Lao Democratic People's Republic.
Singhalath S
Wildlife Conservation Society, Lao Democratic People's Republic.
Greatorex Z
Wildlife Conservation Society, Lao Democratic People's Republic.
Fine AE
Wildlife Conservation Society, Vietnam Program, Hanoi, Vietnam.
Goldstein T
One Health Institute, School of Veterinary Medicine, University of California, Davis, USA.
Olson S
Wildlife Conservation Society, Wildlife Health Program, Bronx, New York, USA.
Joly DO
Wildlife Conservation Society, Wildlife Health Program, Bronx, New York, USA; Metabiota Inc., Nanaimo, British Columbia, Canada.
Keatts L
Wildlife Conservation Society, Cambodia.
Dussart P
Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
Afelt A
Institute of Physical Geography, Faculty of Geography and Regional Studies, University of Warsaw, Warsaw, Poland.
Frutos R
Cirad, UMR 17, Cirad-Ird, TA-A17/G, Montpellier, France; Université de Montpellier, IES, UMR 5214, CNRS-UM, Montpellier, France. Electronic address: roger.frutos@ies.univ-montp2.fr.
Buchy P
Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia; GSK Vaccines R&D, 150 Beach road, # 22-00, 189720, Singapore. Electronic address: buchyphilippe@hotmail.com.

MeSH

AnimalsBase SequenceCambodiaChiropteraCoronavirusDisease ReservoirsEvolution, MolecularGenes, ViralGenetic VariationLaosPhylogenyPhylogeographyRNA-Dependent RNA PolymeraseSequence Analysis, DNAViral Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27932284

Clinical Trial Links

Epidemiological Investigation of COVID-19 in Laos Using a One-Health Approach in Human and Animals

Citation

Lacroix, Audrey, et al. "Genetic Diversity of Coronaviruses in Bats in Lao PDR and Cambodia." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 48, 2017, pp. 10-18.
Lacroix A, Duong V, Hul V, et al. Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia. Infect Genet Evol. 2017;48:10-18.
Lacroix, A., Duong, V., Hul, V., San, S., Davun, H., Omaliss, K., Chea, S., Hassanin, A., Theppangna, W., Silithammavong, S., Khammavong, K., Singhalath, S., Greatorex, Z., Fine, A. E., Goldstein, T., Olson, S., Joly, D. O., Keatts, L., Dussart, P., ... Buchy, P. (2017). Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 48, 10-18. https://doi.org/10.1016/j.meegid.2016.11.029
Lacroix A, et al. Genetic Diversity of Coronaviruses in Bats in Lao PDR and Cambodia. Infect Genet Evol. 2017;48:10-18. PubMed PMID: 27932284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia. AU - Lacroix,Audrey, AU - Duong,Veasna, AU - Hul,Vibol, AU - San,Sorn, AU - Davun,Hull, AU - Omaliss,Keo, AU - Chea,Sokha, AU - Hassanin,Alexandre, AU - Theppangna,Watthana, AU - Silithammavong,Soubanh, AU - Khammavong,Kongsy, AU - Singhalath,Sinpakone, AU - Greatorex,Zoe, AU - Fine,Amanda E, AU - Goldstein,Tracey, AU - Olson,Sarah, AU - Joly,Damien O, AU - Keatts,Lucy, AU - Dussart,Philippe, AU - Afelt,Aneta, AU - Frutos,Roger, AU - Buchy,Philippe, Y1 - 2016/12/06/ PY - 2016/08/01/received PY - 2016/11/26/revised PY - 2016/11/26/accepted PY - 2016/12/10/pubmed PY - 2017/11/4/medline PY - 2016/12/10/entrez KW - Bats KW - Cambodia KW - Coronaviruses KW - Genetic diversity KW - Lao PDR SP - 10 EP - 18 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 48 N2 - South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n=55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/27932284/Genetic_diversity_of_coronaviruses_in_bats_in_Lao_PDR_and_Cambodia_ DB - PRIME DP - Unbound Medicine ER -