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Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats.
J Physiol Biochem. 2017 May; 73(2):207-214.JP

Abstract

Vasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perfusion (MBP and CBP respectively) in both stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar rats. CBP and MBP were measured using a laser-Doppler flow meter before and after intra-renal infusion of tempol, the superoxide dismutase (SOD) mimetic or tempol plus catalase, the hydrogen peroxide-degrading enzyme. Tempol infusion significantly elevated blood perfusion within the renal medulla (MBP) in both SHRSP (by 43 ± 7%, P < 0.001) and Wistar rats (by 17 ± 2%, P < 0.05) but the magnitude of the increase was significantly greater in the SHRSP (P < 0.01). When the enzyme catalase and tempol were co-infused, MBP was again significantly increased in SHRSP (by 57 ± 6%, P < 0.001) and Wistar rats (by 33 ± 6%, P < 0.001), with a significantly greater increase in perfusion being induced in the SHRSP relative to the Wistar rats (P < 0.01). Notably, this increase was significantly greater than in those animals infused with tempol alone (P < 0.01). These results suggest that ROS plays a proportionally greater role in reducing renal vascular compliance, particularly within the renal medulla, in normotensive and hypertensive animals, with effects being greater in the hypertensive animals. This supports the hypothesis that SHRSP renal vasculature might be subjected to elevated level of oxidative stress relative to normotensive animals.

Authors+Show Affiliations

Department of Physiology, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia. aahmeda@ksu.edu.sa.Department of Physiology, University College Cork, Cork, Republic of, Ireland.Department of Physiology, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia.Department of Physiology, University College Cork, Cork, Republic of, Ireland.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27933463

Citation

Ahmeda, Ahmad F., et al. "Effect of Tempol and Tempol Plus Catalase On Intra-renal Haemodynamics in Spontaneously Hypertensive Stroke-prone (SHSP) and Wistar Rats." Journal of Physiology and Biochemistry, vol. 73, no. 2, 2017, pp. 207-214.
Ahmeda AF, Rae MG, Al Otaibi MF, et al. Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats. J Physiol Biochem. 2017;73(2):207-214.
Ahmeda, A. F., Rae, M. G., Al Otaibi, M. F., Anweigi, L. M., & Johns, E. J. (2017). Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats. Journal of Physiology and Biochemistry, 73(2), 207-214. https://doi.org/10.1007/s13105-016-0541-1
Ahmeda AF, et al. Effect of Tempol and Tempol Plus Catalase On Intra-renal Haemodynamics in Spontaneously Hypertensive Stroke-prone (SHSP) and Wistar Rats. J Physiol Biochem. 2017;73(2):207-214. PubMed PMID: 27933463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats. AU - Ahmeda,Ahmad F, AU - Rae,Mark G, AU - Al Otaibi,Mohammed F, AU - Anweigi,Lamyia M, AU - Johns,Edward J, Y1 - 2016/12/09/ PY - 2016/09/05/received PY - 2016/11/24/accepted PY - 2016/12/10/pubmed PY - 2017/10/5/medline PY - 2016/12/10/entrez KW - Catalase KW - Reactive oxygen species KW - Renal blood perfusion KW - SHRSP KW - Tempol SP - 207 EP - 214 JF - Journal of physiology and biochemistry JO - J Physiol Biochem VL - 73 IS - 2 N2 - Vasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perfusion (MBP and CBP respectively) in both stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar rats. CBP and MBP were measured using a laser-Doppler flow meter before and after intra-renal infusion of tempol, the superoxide dismutase (SOD) mimetic or tempol plus catalase, the hydrogen peroxide-degrading enzyme. Tempol infusion significantly elevated blood perfusion within the renal medulla (MBP) in both SHRSP (by 43 ± 7%, P < 0.001) and Wistar rats (by 17 ± 2%, P < 0.05) but the magnitude of the increase was significantly greater in the SHRSP (P < 0.01). When the enzyme catalase and tempol were co-infused, MBP was again significantly increased in SHRSP (by 57 ± 6%, P < 0.001) and Wistar rats (by 33 ± 6%, P < 0.001), with a significantly greater increase in perfusion being induced in the SHRSP relative to the Wistar rats (P < 0.01). Notably, this increase was significantly greater than in those animals infused with tempol alone (P < 0.01). These results suggest that ROS plays a proportionally greater role in reducing renal vascular compliance, particularly within the renal medulla, in normotensive and hypertensive animals, with effects being greater in the hypertensive animals. This supports the hypothesis that SHRSP renal vasculature might be subjected to elevated level of oxidative stress relative to normotensive animals. SN - 1877-8755 UR - https://www.unboundmedicine.com/medline/citation/27933463/Effect_of_tempol_and_tempol_plus_catalase_on_intra_renal_haemodynamics_in_spontaneously_hypertensive_stroke_prone__SHSP__and_Wistar_rats_ DB - PRIME DP - Unbound Medicine ER -