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Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability.
Langmuir. 2016 12 06; 32(48):12894-12904.L

Abstract

Omega-O-acylceramides (acylCer), a subclass of sphingolipids with an ultralong N-acyl chain (from 20 to 38 carbons, most usually 30 and 32 carbons), are crucial components of the skin permeability barrier. AcylCer are involved in the formation of the long periodicity lamellar phase (LPP, 12-13 nm), which is essential for preventing water loss from the body. Lower levels of acylCer and LPP accompany skin diseases, such as atopic dermatitis, lamellar ichthyosis, and psoriasis. We studied how the concentration and structure of acylCer influence the organization and permeability barrier properties of model lipid membranes. For simple model membranes composed of the sphingosine-containing acylCer (EOS), N-lignoceroyl sphingosine, lignoceric acid, cholesterol (Chol), and cholesteryl sulfate (CholS), the LPP formed at 10% Cer EOS (of the total Cer) and the short periodicity phase disappeared at 30% Cer EOS. Surprisingly, membranes with the LPP had higher permeabilities than the control membrane without acylCer. In the complex models consisting of acylCer (EOS, phytosphingosine EOP, dihydrosphingosine EOdS, or their mixture; at 10% of the total Cer), a six-component Cer mixture, a free fatty acid mixture, cholesterol (Chol), and cholesteryl sulfate (CholS), acylCer decreased the membrane permeability to model permeants (with the strongest effects for acylCer EOP and EOdS) when compared with the permeability of the control membrane without acylCer. However, in the complex model, only a mixture of acylCer EOS, EOdS, and EOP and not the individual acylCer formed both the LPP and orthorhombic chain packing at the 10% level. Thus, the relationships between acylCer, LPP formation, and permeability barrier function are not trivial. Lipid heterogeneity is essential-only the most complex model with nine Cer subclasses mimicked both the organization and permeability of stratum corneum lipid membranes.

Authors+Show Affiliations

Faculty of Pharmacy, Charles University , Hradec Králové 500 05, Czech Republic.Faculty of Pharmacy, Charles University , Hradec Králové 500 05, Czech Republic.University of Chemistry and Technology Prague , Prague 166 28, Czech Republic.Faculty of Pharmacy, Charles University , Hradec Králové 500 05, Czech Republic.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27934529

Citation

Opálka, Lukáš, et al. "Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity On Microstructure and Permeability." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 32, no. 48, 2016, pp. 12894-12904.
Opálka L, Kováčik A, Maixner J, et al. Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability. Langmuir. 2016;32(48):12894-12904.
Opálka, L., Kováčik, A., Maixner, J., & Vávrová, K. (2016). Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability. Langmuir : the ACS Journal of Surfaces and Colloids, 32(48), 12894-12904.
Opálka L, et al. Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity On Microstructure and Permeability. Langmuir. 2016 12 6;32(48):12894-12904. PubMed PMID: 27934529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability. AU - Opálka,Lukáš, AU - Kováčik,Andrej, AU - Maixner,Jaroslav, AU - Vávrová,Kateřina, Y1 - 2016/11/23/ PY - 2016/12/10/entrez PY - 2016/12/10/pubmed PY - 2018/9/19/medline SP - 12894 EP - 12904 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 32 IS - 48 N2 - Omega-O-acylceramides (acylCer), a subclass of sphingolipids with an ultralong N-acyl chain (from 20 to 38 carbons, most usually 30 and 32 carbons), are crucial components of the skin permeability barrier. AcylCer are involved in the formation of the long periodicity lamellar phase (LPP, 12-13 nm), which is essential for preventing water loss from the body. Lower levels of acylCer and LPP accompany skin diseases, such as atopic dermatitis, lamellar ichthyosis, and psoriasis. We studied how the concentration and structure of acylCer influence the organization and permeability barrier properties of model lipid membranes. For simple model membranes composed of the sphingosine-containing acylCer (EOS), N-lignoceroyl sphingosine, lignoceric acid, cholesterol (Chol), and cholesteryl sulfate (CholS), the LPP formed at 10% Cer EOS (of the total Cer) and the short periodicity phase disappeared at 30% Cer EOS. Surprisingly, membranes with the LPP had higher permeabilities than the control membrane without acylCer. In the complex models consisting of acylCer (EOS, phytosphingosine EOP, dihydrosphingosine EOdS, or their mixture; at 10% of the total Cer), a six-component Cer mixture, a free fatty acid mixture, cholesterol (Chol), and cholesteryl sulfate (CholS), acylCer decreased the membrane permeability to model permeants (with the strongest effects for acylCer EOP and EOdS) when compared with the permeability of the control membrane without acylCer. However, in the complex model, only a mixture of acylCer EOS, EOdS, and EOP and not the individual acylCer formed both the LPP and orthorhombic chain packing at the 10% level. Thus, the relationships between acylCer, LPP formation, and permeability barrier function are not trivial. Lipid heterogeneity is essential-only the most complex model with nine Cer subclasses mimicked both the organization and permeability of stratum corneum lipid membranes. SN - 1520-5827 UR - https://www.unboundmedicine.com/medline/citation/27934529/Omega_O_Acylceramides_in_Skin_Lipid_Membranes:_Effects_of_Concentration_Sphingoid_Base_and_Model_Complexity_on_Microstructure_and_Permeability_ L2 - https://doi.org/10.1021/acs.langmuir.6b03082 DB - PRIME DP - Unbound Medicine ER -
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