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Cooperative synthesis of dopamine by non-dopaminergic neurons as a compensatory mechanism in the striatum of mice with MPTP-induced Parkinsonism.
Neurobiol Dis. 2017 Feb; 98:108-121.ND

Abstract

Since the late 80s it has been repeatedly shown that besides dopaminergic neurons, the brain contains so-called monoenzymatic neurons possessing one of the enzymes of dopamine (DA) synthesis, tyrosine hydroxylase (TH) or aromatic l-amino acid decarboxylase (AADC). However, the data on the existence of monoenzymatic neurons in the striatum remain controversial, and little is known about their functional significance. The aim of this study was to test our hypothesis that monoenzymatic TH-containing neurons produce DA in cooperation with the neurons containing AADC, which might help to compensate DA deficiency under the failure of the nigrostriatal dopaminergic system. Using a combination of techniques: retrograde tracing, qPCR and immunolabeling for TH, AADC and MAP2, we showed that the striatum of mice with normal and degraded dopaminergic system comprises of monoenzymatic TH- and AADC-containing neurons. To provide evidence for cooperative synthesis of DA, we used an ex vivo model of inhibiting of DA synthesis by blocking transport of l-DOPA, produced in monoenzymatic TH-containing neurons, to neurons containing AADC by means of l-leucine, a competitive inhibitor of the membrane transporter of large neutral amino acids, and l-DOPA. With this original approach, cooperative synthesis of DA in the striatum was proven in MPTP-treated mice but not in the control. Furthermore, we demonstrated that the proportion of DA produced through cooperative synthesis in the striatum of MPTP-treated mice increases as the degradation of dopaminergic system proceeds. An increase in the proportion of cooperative synthesis of DA alongside degradation of the dopaminergic system is also proved by an increase of both TH gene expression and the number of TH-immunoreactive structures in the striatum. Thus, these data suggest that the cooperative synthesis of DA in the degraded striatum is an up-regulated compensatory reaction, which plays an increasing role as DA deficiency rises, and might be considered among the principal mechanisms of neuroplasticity in neurodegenerative diseases.

Authors+Show Affiliations

Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology RAS, 26 Vavilov St, Moscow 119334, Russia.Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology RAS, 26 Vavilov St, Moscow 119334, Russia.Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology RAS, 26 Vavilov St, Moscow 119334, Russia.Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology RAS, 26 Vavilov St, Moscow 119334, Russia; Department of Psychology, Faculty of Social Sciences, The National Research University Higher School of Economics, 20 Myasnitskaya St, Moscow 101000, Russia. Electronic address: michael.ugrumov@mail.ru.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27940203

Citation

Kozina, Elena A., et al. "Cooperative Synthesis of Dopamine By Non-dopaminergic Neurons as a Compensatory Mechanism in the Striatum of Mice With MPTP-induced Parkinsonism." Neurobiology of Disease, vol. 98, 2017, pp. 108-121.
Kozina EA, Kim AR, Kurina AY, et al. Cooperative synthesis of dopamine by non-dopaminergic neurons as a compensatory mechanism in the striatum of mice with MPTP-induced Parkinsonism. Neurobiol Dis. 2017;98:108-121.
Kozina, E. A., Kim, A. R., Kurina, A. Y., & Ugrumov, M. V. (2017). Cooperative synthesis of dopamine by non-dopaminergic neurons as a compensatory mechanism in the striatum of mice with MPTP-induced Parkinsonism. Neurobiology of Disease, 98, 108-121. https://doi.org/10.1016/j.nbd.2016.12.005
Kozina EA, et al. Cooperative Synthesis of Dopamine By Non-dopaminergic Neurons as a Compensatory Mechanism in the Striatum of Mice With MPTP-induced Parkinsonism. Neurobiol Dis. 2017;98:108-121. PubMed PMID: 27940203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cooperative synthesis of dopamine by non-dopaminergic neurons as a compensatory mechanism in the striatum of mice with MPTP-induced Parkinsonism. AU - Kozina,Elena A, AU - Kim,Aleksandr R, AU - Kurina,Anna Y, AU - Ugrumov,Michael V, Y1 - 2016/12/06/ PY - 2016/07/29/received PY - 2016/12/01/revised PY - 2016/12/02/accepted PY - 2016/12/13/pubmed PY - 2018/2/28/medline PY - 2016/12/13/entrez KW - 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine KW - Aromatic l-amino acid decarboxylase KW - Mice KW - Nigrostriatal system KW - Parkinson's disease KW - Tyrosine hydroxylase SP - 108 EP - 121 JF - Neurobiology of disease JO - Neurobiol Dis VL - 98 N2 - Since the late 80s it has been repeatedly shown that besides dopaminergic neurons, the brain contains so-called monoenzymatic neurons possessing one of the enzymes of dopamine (DA) synthesis, tyrosine hydroxylase (TH) or aromatic l-amino acid decarboxylase (AADC). However, the data on the existence of monoenzymatic neurons in the striatum remain controversial, and little is known about their functional significance. The aim of this study was to test our hypothesis that monoenzymatic TH-containing neurons produce DA in cooperation with the neurons containing AADC, which might help to compensate DA deficiency under the failure of the nigrostriatal dopaminergic system. Using a combination of techniques: retrograde tracing, qPCR and immunolabeling for TH, AADC and MAP2, we showed that the striatum of mice with normal and degraded dopaminergic system comprises of monoenzymatic TH- and AADC-containing neurons. To provide evidence for cooperative synthesis of DA, we used an ex vivo model of inhibiting of DA synthesis by blocking transport of l-DOPA, produced in monoenzymatic TH-containing neurons, to neurons containing AADC by means of l-leucine, a competitive inhibitor of the membrane transporter of large neutral amino acids, and l-DOPA. With this original approach, cooperative synthesis of DA in the striatum was proven in MPTP-treated mice but not in the control. Furthermore, we demonstrated that the proportion of DA produced through cooperative synthesis in the striatum of MPTP-treated mice increases as the degradation of dopaminergic system proceeds. An increase in the proportion of cooperative synthesis of DA alongside degradation of the dopaminergic system is also proved by an increase of both TH gene expression and the number of TH-immunoreactive structures in the striatum. Thus, these data suggest that the cooperative synthesis of DA in the degraded striatum is an up-regulated compensatory reaction, which plays an increasing role as DA deficiency rises, and might be considered among the principal mechanisms of neuroplasticity in neurodegenerative diseases. SN - 1095-953X UR - https://www.unboundmedicine.com/medline/citation/27940203/Cooperative_synthesis_of_dopamine_by_non_dopaminergic_neurons_as_a_compensatory_mechanism_in_the_striatum_of_mice_with_MPTP_induced_Parkinsonism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-9961(16)30286-8 DB - PRIME DP - Unbound Medicine ER -