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Molecular diversity of α-gliadin expressed genes in genetically contrasted spelt (Triticum aestivum ssp. spelta) accessions and comparison with bread wheat (T. aestivum ssp. aestivum) and related diploid Triticum and Aegilops species.
Mol Breed. 2016; 36(11):152.MB

Abstract

The gluten proteins of cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta) are responsible for celiac disease (CD). The α-gliadins constitute the most immunogenic class of gluten proteins as they include four main T-cell stimulatory epitopes that affect CD patients. Spelt has been less studied than bread wheat and could constitute a source of valuable diversity. The objective of this work was to study the genetic diversity of spelt α-gliadin transcripts and to compare it with those of bread wheat. Genotyping data from 85 spelt accessions obtained with 19 simple sequence repeat (SSR) markers were used to select 11 contrasted accessions, from which 446 full open reading frame α-gliadin genes were cloned and sequenced, which revealed a high allelic diversity. High variations among the accessions were highlighted, in terms of the proportion of α-gliadin sequences from each of the three genomes (A, B and D), and their composition in the four T-cell stimulatory epitopes. An accession from Tajikistan stood out, having a particularly high proportion of α-gliadins from the B genome and a low immunogenic content. Even if no clear separation between spelt and bread wheat sequences was shown, spelt α-gliadins displayed specific features concerning e.g. the frequencies of some amino acid substitutions. Given this observation and the variations in toxicity revealed in the spelt accessions in this study, the high genetic diversity held in spelt germplasm collections could be a valuable resource in the development of safer varieties for CD patients.

Authors+Show Affiliations

Centre wallon de Recherches agronomiques (CRA-W), Département Sciences du vivant, Chaussée de Charleroi, 234, 5030 Gembloux, Belgium ; Earth and Life Institute - Agronomy, Université catholique de Louvain (UCL), Croix du Sud, 2 bte L7.05.11, 1348 Louvain-la-Neuve, Belgium.Earth and Life Institute - Agronomy, Université catholique de Louvain (UCL), Croix du Sud, 2 bte L7.05.11, 1348 Louvain-la-Neuve, Belgium.Centre wallon de Recherches agronomiques (CRA-W), Département Sciences du vivant, Chaussée de Charleroi, 234, 5030 Gembloux, Belgium.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27942245

Citation

Dubois, Benjamin, et al. "Molecular Diversity of Α-gliadin Expressed Genes in Genetically Contrasted Spelt (Triticum Aestivum Ssp. Spelta) Accessions and Comparison With Bread Wheat (T. Aestivum Ssp. Aestivum) and Related Diploid Triticum and Aegilops Species." Molecular Breeding : New Strategies in Plant Improvement, vol. 36, no. 11, 2016, p. 152.
Dubois B, Bertin P, Mingeot D. Molecular diversity of α-gliadin expressed genes in genetically contrasted spelt (Triticum aestivum ssp. spelta) accessions and comparison with bread wheat (T. aestivum ssp. aestivum) and related diploid Triticum and Aegilops species. Mol Breed. 2016;36(11):152.
Dubois, B., Bertin, P., & Mingeot, D. (2016). Molecular diversity of α-gliadin expressed genes in genetically contrasted spelt (Triticum aestivum ssp. spelta) accessions and comparison with bread wheat (T. aestivum ssp. aestivum) and related diploid Triticum and Aegilops species. Molecular Breeding : New Strategies in Plant Improvement, 36(11), 152.
Dubois B, Bertin P, Mingeot D. Molecular Diversity of Α-gliadin Expressed Genes in Genetically Contrasted Spelt (Triticum Aestivum Ssp. Spelta) Accessions and Comparison With Bread Wheat (T. Aestivum Ssp. Aestivum) and Related Diploid Triticum and Aegilops Species. Mol Breed. 2016;36(11):152. PubMed PMID: 27942245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular diversity of α-gliadin expressed genes in genetically contrasted spelt (Triticum aestivum ssp. spelta) accessions and comparison with bread wheat (T. aestivum ssp. aestivum) and related diploid Triticum and Aegilops species. AU - Dubois,Benjamin, AU - Bertin,Pierre, AU - Mingeot,Dominique, Y1 - 2016/11/10/ PY - 2016/05/13/received PY - 2016/10/11/accepted PY - 2016/12/13/entrez PY - 2016/12/13/pubmed PY - 2016/12/13/medline KW - Celiac disease KW - Genetic diversity KW - Gluten KW - Spelt KW - α-gliadin SP - 152 EP - 152 JF - Molecular breeding : new strategies in plant improvement JO - Mol Breed VL - 36 IS - 11 N2 - The gluten proteins of cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta) are responsible for celiac disease (CD). The α-gliadins constitute the most immunogenic class of gluten proteins as they include four main T-cell stimulatory epitopes that affect CD patients. Spelt has been less studied than bread wheat and could constitute a source of valuable diversity. The objective of this work was to study the genetic diversity of spelt α-gliadin transcripts and to compare it with those of bread wheat. Genotyping data from 85 spelt accessions obtained with 19 simple sequence repeat (SSR) markers were used to select 11 contrasted accessions, from which 446 full open reading frame α-gliadin genes were cloned and sequenced, which revealed a high allelic diversity. High variations among the accessions were highlighted, in terms of the proportion of α-gliadin sequences from each of the three genomes (A, B and D), and their composition in the four T-cell stimulatory epitopes. An accession from Tajikistan stood out, having a particularly high proportion of α-gliadins from the B genome and a low immunogenic content. Even if no clear separation between spelt and bread wheat sequences was shown, spelt α-gliadins displayed specific features concerning e.g. the frequencies of some amino acid substitutions. Given this observation and the variations in toxicity revealed in the spelt accessions in this study, the high genetic diversity held in spelt germplasm collections could be a valuable resource in the development of safer varieties for CD patients. SN - 1380-3743 UR - https://www.unboundmedicine.com/medline/citation/27942245/Molecular_diversity_of_α_gliadin_expressed_genes_in_genetically_contrasted_spelt__Triticum_aestivum_ssp__spelta__accessions_and_comparison_with_bread_wheat__T__aestivum_ssp__aestivum__and_related_diploid_Triticum_and_Aegilops_species_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27942245/ DB - PRIME DP - Unbound Medicine ER -
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