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Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection.
J Forensic Sci. 2017 May; 62(3):602-611.JF

Abstract

Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆8 -THC, THCV, ∆9 -THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples.

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Authors+Show Affiliations

Wang M
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
Wang YH
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
Avula B
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
Radwan MM
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA. Department of Pharmacognosy, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.
Wanas AS
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA. Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.
Mehmedic Z
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
van Antwerp J
Waters Corporation, Milford, MA, 01757, USA.
ElSohly MA
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA. Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
Khan IA
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA. Division of Pharmacognosy, Department of BioMolecular Science, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

MeSH

CannabinoidsCannabisChromatography, Supercritical FluidHumansLeast-Squares AnalysisLimit of DetectionMass SpectrometryMolecular StructurePlant ExtractsPrincipal Component Analysis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27957741

Citation

Wang, Mei, et al. "Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection." Journal of Forensic Sciences, vol. 62, no. 3, 2017, pp. 602-611.
Wang M, Wang YH, Avula B, et al. Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection. J Forensic Sci. 2017;62(3):602-611.
Wang, M., Wang, Y. H., Avula, B., Radwan, M. M., Wanas, A. S., Mehmedic, Z., van Antwerp, J., ElSohly, M. A., & Khan, I. A. (2017). Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection. Journal of Forensic Sciences, 62(3), 602-611. https://doi.org/10.1111/1556-4029.13341
Wang M, et al. Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection. J Forensic Sci. 2017;62(3):602-611. PubMed PMID: 27957741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection. AU - Wang,Mei, AU - Wang,Yan-Hong, AU - Avula,Bharathi, AU - Radwan,Mohamed M, AU - Wanas,Amira S, AU - Mehmedic,Zlatko, AU - van Antwerp,John, AU - ElSohly,Mahmoud A, AU - Khan,Ikhlas A, Y1 - 2016/12/13/ PY - 2016/01/02/received PY - 2016/08/07/revised PY - 2016/08/22/accepted PY - 2016/12/14/pubmed PY - 2018/4/12/medline PY - 2016/12/14/entrez KW - Cannabis sativa L. KW - UHPSFC/PDA-MS KW - cannabinoids KW - chemometric analysis KW - forensic science KW - quantification and method validation SP - 602 EP - 611 JF - Journal of forensic sciences JO - J Forensic Sci VL - 62 IS - 3 N2 - Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆8 -THC, THCV, ∆9 -THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/27957741/Quantitative_Determination_of_Cannabinoids_in_Cannabis_and_Cannabis_Products_Using_Ultra_High_Performance_Supercritical_Fluid_Chromatography_and_Diode_Array/Mass_Spectrometric_Detection_ DB - PRIME DP - Unbound Medicine ER -