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Effect of momentary stress on brain energy metabolism in weanling mice: apparent use of lactate as cerebral metabolic fuel concomitant with a decrease in brain glucose utilization.
Metab Brain Dis. 1989 Sep; 4(3):177-86.MB

Abstract

The hypothesis that the anxiety induced by repeated injections affects brain energy metabolism was tested. Normal 19- to 21-day-old mice were stressed by two sham intraperitoneal injections within 4 min, at which time they were decapitated. Noninjected, control littermates were quickly decapitated. Momentary stress increased plasma glucose (12%), glycerol (85%), beta-hydroxybutyrate (108%), and lactate (153%)--a reflection of elevated plasma cortisol (25%) and glucagon (45%). In brain, stress increased levels of glucose-6-P (15%) and fructose-6-P (17%). The brain pyruvate concentration increased 74%; lactate 76%. Citrate, alpha-ketoglutarate, and malate increased 15, 95, and 37%, respectively. Levels of glycogen, glucose, phosphocreatine, ATP, ADP, and AMP were unchanged. The brain lactate/pyruvate ratio was normal but the brain/plasma lactate ratio fell 32%. Metabolite changes in the stressed animals were compatible with a decrease in the glycolytic flux at the phosphofructokinase step and a paradoxical increased flux in the Krebs citric acid cycle. The decreased brain/plasma lactate ratio supported increased uptake of lactate from plasma and increased brain lactate oxidation. Metabolite changes similar to those described above occurred in unstressed mice injected with lactate. Findings confirm a positive effect of stress on brain metabolism, support a role for lactate as an oxidative fuel for brain, and caution that the rate of cerebral glucose utilization may not always reflect brain energy (oxidative) metabolism accurately.

Authors+Show Affiliations

Washington University School of Medicine, Edward Mallinckrodt Department of Pediatrics, St. Louis Children's Hospital, Missouri 63110.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2796872

Citation

Thurston, J H., and R E. Hauhart. "Effect of Momentary Stress On Brain Energy Metabolism in Weanling Mice: Apparent Use of Lactate as Cerebral Metabolic Fuel Concomitant With a Decrease in Brain Glucose Utilization." Metabolic Brain Disease, vol. 4, no. 3, 1989, pp. 177-86.
Thurston JH, Hauhart RE. Effect of momentary stress on brain energy metabolism in weanling mice: apparent use of lactate as cerebral metabolic fuel concomitant with a decrease in brain glucose utilization. Metab Brain Dis. 1989;4(3):177-86.
Thurston, J. H., & Hauhart, R. E. (1989). Effect of momentary stress on brain energy metabolism in weanling mice: apparent use of lactate as cerebral metabolic fuel concomitant with a decrease in brain glucose utilization. Metabolic Brain Disease, 4(3), 177-86.
Thurston JH, Hauhart RE. Effect of Momentary Stress On Brain Energy Metabolism in Weanling Mice: Apparent Use of Lactate as Cerebral Metabolic Fuel Concomitant With a Decrease in Brain Glucose Utilization. Metab Brain Dis. 1989;4(3):177-86. PubMed PMID: 2796872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of momentary stress on brain energy metabolism in weanling mice: apparent use of lactate as cerebral metabolic fuel concomitant with a decrease in brain glucose utilization. AU - Thurston,J H, AU - Hauhart,R E, PY - 1989/9/1/pubmed PY - 1989/9/1/medline PY - 1989/9/1/entrez SP - 177 EP - 86 JF - Metabolic brain disease JO - Metab Brain Dis VL - 4 IS - 3 N2 - The hypothesis that the anxiety induced by repeated injections affects brain energy metabolism was tested. Normal 19- to 21-day-old mice were stressed by two sham intraperitoneal injections within 4 min, at which time they were decapitated. Noninjected, control littermates were quickly decapitated. Momentary stress increased plasma glucose (12%), glycerol (85%), beta-hydroxybutyrate (108%), and lactate (153%)--a reflection of elevated plasma cortisol (25%) and glucagon (45%). In brain, stress increased levels of glucose-6-P (15%) and fructose-6-P (17%). The brain pyruvate concentration increased 74%; lactate 76%. Citrate, alpha-ketoglutarate, and malate increased 15, 95, and 37%, respectively. Levels of glycogen, glucose, phosphocreatine, ATP, ADP, and AMP were unchanged. The brain lactate/pyruvate ratio was normal but the brain/plasma lactate ratio fell 32%. Metabolite changes in the stressed animals were compatible with a decrease in the glycolytic flux at the phosphofructokinase step and a paradoxical increased flux in the Krebs citric acid cycle. The decreased brain/plasma lactate ratio supported increased uptake of lactate from plasma and increased brain lactate oxidation. Metabolite changes similar to those described above occurred in unstressed mice injected with lactate. Findings confirm a positive effect of stress on brain metabolism, support a role for lactate as an oxidative fuel for brain, and caution that the rate of cerebral glucose utilization may not always reflect brain energy (oxidative) metabolism accurately. SN - 0885-7490 UR - https://www.unboundmedicine.com/medline/citation/2796872/Effect_of_momentary_stress_on_brain_energy_metabolism_in_weanling_mice:_apparent_use_of_lactate_as_cerebral_metabolic_fuel_concomitant_with_a_decrease_in_brain_glucose_utilization_ DB - PRIME DP - Unbound Medicine ER -