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SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato.
Plant Sci. 2016 Dec; 253:13-20.PS

Abstract

TIR1 and its homologs act as auxin receptors and play important roles in plant growth and development in Arabidopsis thaliana. An auxin receptor homolog Solanum lycopersicum TIR1 (SlTIR1) has been isolated from tomato cultivar Micro-Tom, and SlTIR1 over-expression results in parthenocarpic fruit formation. In this study, the promoter driving the β-glucuronidase (GUS) expression vector was constructed and stably transformed into Micro-Tom seedlings. The SlTIR1 promoter driving GUS expression accumulated predominantly in the leaf and vasculature in transgenic seedlings. Promoter analysis identified an auxin-response element (AuxRE) and two gibberellic acid (GA)-response elements in the SlTIR1 promoter. Quantitative PCR showed that SlTIR1 transcript level was down-regulated by naphthaleneacetic acid, ethephon and abscisic acid and up-regulated by GA. Furthermore, because of the lack of ability to form reproductive seeds in SlTIR1 over-expressing Micro-Tom, this limits further exploration of potential roles of SlTIR1 in auxin signaling. Here, an antisense vector and an over-expression vector of the SlTIR1 gene were stably transformed into Micro-Tom and Ailsa Craig tomato, respectively. Phenotypes and physiological analyses indicated that SlTIR1 regulated primary root growth and auxin-associated lateral root formation in Micro-Tom. Meanwhile, SlTIR1 also stimulated abnormal seed development, so-called stenospermocarpy, in Ailsa Craig. Transcript accumulations of auxin-signaling genes determined by quantitative PCR were consistent with the idea that SlTIR1 regulated plant growth and development, partially mediated by controlling the mRNA levels of auxin-signaling genes. Our work demonstrates that SlTIR1 regulated auxin-induced root growth and stimulated stenospermocarpic fruit formation. SlTIR1 may be a key mediator of the crosstalk among auxin and other hormones to co-regulate plant growth and development.

Authors+Show Affiliations

Department of Space Radiobiology, Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China. Electronic address: zhxren@impcas.ac.cn.College of Biology & Pharmacy, Yulin Normal University, Yulin 537000, China. Electronic address: wxmin99@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27968981

Citation

Ren, Zhenxin, and Xiaomin Wang. "SlTIR1 Is Involved in Crosstalk of Phytohormones, Regulates Auxin-induced Root Growth and Stimulates Stenospermocarpic Fruit Formation in Tomato." Plant Science : an International Journal of Experimental Plant Biology, vol. 253, 2016, pp. 13-20.
Ren Z, Wang X. SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato. Plant Sci. 2016;253:13-20.
Ren, Z., & Wang, X. (2016). SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato. Plant Science : an International Journal of Experimental Plant Biology, 253, 13-20. https://doi.org/10.1016/j.plantsci.2016.09.005
Ren Z, Wang X. SlTIR1 Is Involved in Crosstalk of Phytohormones, Regulates Auxin-induced Root Growth and Stimulates Stenospermocarpic Fruit Formation in Tomato. Plant Sci. 2016;253:13-20. PubMed PMID: 27968981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato. AU - Ren,Zhenxin, AU - Wang,Xiaomin, Y1 - 2016/09/13/ PY - 2016/06/02/received PY - 2016/09/12/revised PY - 2016/09/13/accepted PY - 2016/12/15/entrez PY - 2016/12/15/pubmed PY - 2017/5/10/medline KW - Auxin signaling KW - Hormone crosstalk KW - Root formation KW - SlTIR1 KW - Stenospermocarpic fruit SP - 13 EP - 20 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 253 N2 - TIR1 and its homologs act as auxin receptors and play important roles in plant growth and development in Arabidopsis thaliana. An auxin receptor homolog Solanum lycopersicum TIR1 (SlTIR1) has been isolated from tomato cultivar Micro-Tom, and SlTIR1 over-expression results in parthenocarpic fruit formation. In this study, the promoter driving the β-glucuronidase (GUS) expression vector was constructed and stably transformed into Micro-Tom seedlings. The SlTIR1 promoter driving GUS expression accumulated predominantly in the leaf and vasculature in transgenic seedlings. Promoter analysis identified an auxin-response element (AuxRE) and two gibberellic acid (GA)-response elements in the SlTIR1 promoter. Quantitative PCR showed that SlTIR1 transcript level was down-regulated by naphthaleneacetic acid, ethephon and abscisic acid and up-regulated by GA. Furthermore, because of the lack of ability to form reproductive seeds in SlTIR1 over-expressing Micro-Tom, this limits further exploration of potential roles of SlTIR1 in auxin signaling. Here, an antisense vector and an over-expression vector of the SlTIR1 gene were stably transformed into Micro-Tom and Ailsa Craig tomato, respectively. Phenotypes and physiological analyses indicated that SlTIR1 regulated primary root growth and auxin-associated lateral root formation in Micro-Tom. Meanwhile, SlTIR1 also stimulated abnormal seed development, so-called stenospermocarpy, in Ailsa Craig. Transcript accumulations of auxin-signaling genes determined by quantitative PCR were consistent with the idea that SlTIR1 regulated plant growth and development, partially mediated by controlling the mRNA levels of auxin-signaling genes. Our work demonstrates that SlTIR1 regulated auxin-induced root growth and stimulated stenospermocarpic fruit formation. SlTIR1 may be a key mediator of the crosstalk among auxin and other hormones to co-regulate plant growth and development. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/27968981/SlTIR1_is_involved_in_crosstalk_of_phytohormones_regulates_auxin_induced_root_growth_and_stimulates_stenospermocarpic_fruit_formation_in_tomato_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-9452(16)30438-1 DB - PRIME DP - Unbound Medicine ER -