Tags

Type your tag names separated by a space and hit enter

Association of Enteric Protist Blastocystis spp. and Gut Microbiota with Hepatic Encephalopathy.
J Gastrointestin Liver Dis 2016; 25(4):489-497JG

Abstract

BACKGROUND AND AIMS

Hepatic encephalopathy (HE) is a serious neuropsychiatric sequela emerging in the advanced stages of cirrhosis. The gut microbiota plays an important role in the development of HE. The aim of the study was to analyze the dynamic interplay between microbiota and Blastocystis in cirrhotic patients with or without encephalopathy.

METHODS

The study was designed as cross-sectional study. A total of 37 patients from the Ankara city, admitted to the University Hospital within a 6-month period prior to enrolment into the study were included in the study. After the regular health checks, clinical histories, clinical examinations, and Psychometric HE Score (PHES) points, patients' MELD and CTP scores were recorded. The fecal microbiota configurations were characterized by targeting hypervariable regions V3 and V4 of the 16S rRNA gene using Illumina MiSeq System.

RESULTS

Blastocystis spp. were detected in 21.6% (n = 8) of all cirrhotic patients. When those were analyzed by subgroups, four of them were subtype 2, three were subtype 3 and one was subtype 1. Blastocystis spp. were not found in any of the patients with HE; however, they were detected in 38.1% of the patients without HE. Also the increase in the bacterial diversity was observed along with the absence of Blastocystis. It was suggested that there was an inverse relationship between Blastocystis spp. and advanced stages of HE and the structure and composition of gut microbiota.

CONCLUSION

The absence of Blastocystis spp. is associated with the HE severity and dysbiosis in the gut microbiota.

Authors+Show Affiliations

Etimesgut Sait Ertürk State Hospital, Ankara, Turkey. drsedatyildiz06@gmail.com.Section of Gastroenterology, Department of Internal Disease, Faculty of Medicine, Gazi University, Ankara, Turkey.Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Computer Engineering Department,Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey.Medipol University International Faculty of Medicine, Department of Medical Genetics, İstanbul, Turkey.Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Medipol University International School of Medicine, Department of Medical Microbiology, İstanbul, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27981305

Citation

Yildiz, Sedat, et al. "Association of Enteric Protist Blastocystis Spp. and Gut Microbiota With Hepatic Encephalopathy." Journal of Gastrointestinal and Liver Diseases : JGLD, vol. 25, no. 4, 2016, pp. 489-497.
Yildiz S, Doğan İ, Doğruman-Al F, et al. Association of Enteric Protist Blastocystis spp. and Gut Microbiota with Hepatic Encephalopathy. J Gastrointestin Liver Dis. 2016;25(4):489-497.
Yildiz, S., Doğan, İ., Doğruman-Al, F., Nalbantoğlu, U., Üstek, D., Sarzhanov, F., & Yildirim, S. (2016). Association of Enteric Protist Blastocystis spp. and Gut Microbiota with Hepatic Encephalopathy. Journal of Gastrointestinal and Liver Diseases : JGLD, 25(4), pp. 489-497. doi:10.15403/jgld.2014.1121.254.yiz.
Yildiz S, et al. Association of Enteric Protist Blastocystis Spp. and Gut Microbiota With Hepatic Encephalopathy. J Gastrointestin Liver Dis. 2016;25(4):489-497. PubMed PMID: 27981305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of Enteric Protist Blastocystis spp. and Gut Microbiota with Hepatic Encephalopathy. AU - Yildiz,Sedat, AU - Doğan,İbrahim, AU - Doğruman-Al,Funda, AU - Nalbantoğlu,Ufuk, AU - Üstek,Duran, AU - Sarzhanov,Fakhriddin, AU - Yildirim,Süleyman, PY - 2016/12/17/entrez PY - 2016/12/17/pubmed PY - 2017/8/24/medline SP - 489 EP - 497 JF - Journal of gastrointestinal and liver diseases : JGLD JO - J Gastrointestin Liver Dis VL - 25 IS - 4 N2 - BACKGROUND AND AIMS: Hepatic encephalopathy (HE) is a serious neuropsychiatric sequela emerging in the advanced stages of cirrhosis. The gut microbiota plays an important role in the development of HE. The aim of the study was to analyze the dynamic interplay between microbiota and Blastocystis in cirrhotic patients with or without encephalopathy. METHODS: The study was designed as cross-sectional study. A total of 37 patients from the Ankara city, admitted to the University Hospital within a 6-month period prior to enrolment into the study were included in the study. After the regular health checks, clinical histories, clinical examinations, and Psychometric HE Score (PHES) points, patients' MELD and CTP scores were recorded. The fecal microbiota configurations were characterized by targeting hypervariable regions V3 and V4 of the 16S rRNA gene using Illumina MiSeq System. RESULTS: Blastocystis spp. were detected in 21.6% (n = 8) of all cirrhotic patients. When those were analyzed by subgroups, four of them were subtype 2, three were subtype 3 and one was subtype 1. Blastocystis spp. were not found in any of the patients with HE; however, they were detected in 38.1% of the patients without HE. Also the increase in the bacterial diversity was observed along with the absence of Blastocystis. It was suggested that there was an inverse relationship between Blastocystis spp. and advanced stages of HE and the structure and composition of gut microbiota. CONCLUSION: The absence of Blastocystis spp. is associated with the HE severity and dysbiosis in the gut microbiota. SN - 1842-1121 UR - https://www.unboundmedicine.com/medline/citation/27981305/Association_of_Enteric_Protist_Blastocystis_spp__and_Gut_Microbiota_with_Hepatic_Encephalopathy_ L2 - https://dx.doi.org/10.15403/jgld.2014.1121.254.yiz DB - PRIME DP - Unbound Medicine ER -