Tags

Type your tag names separated by a space and hit enter

RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants.
Int J Mol Sci. 2016 Dec 14; 17(12)IJ

Abstract

RNA interference (RNAi) has been widely used in functional genomics of insects and received intensive attention in the development of RNAi-based plants for insect control. Ecdysone receptor (EcR) and ultraspiracle protein (USP) play important roles in molting, metamorphosis, and reproduction of insects. EcR and USP orthologs and their function in grain aphid (Sitobion avenae F.) have not been documented yet. Here, RT-PCR, qRT-PCR, dsRNA feeding assay and aphid bioassay were employed to isolate EcR and USP orthologs in grain aphid, investigate their expression patterns, and evaluate the effect of RNAi on aphid survival and fecundity, and its persistence. The results indicated that SaEcR and SaUSP exhibited similar expression profiles at different developmental stages. Oral administration of dsRNAs of SaEcR and dsSaUSP significantly decreased the survival of aphids due to the down-regulation of these two genes, respectively. The silencing effect was persistent and transgenerational, as demonstrated by the reduced survival and fecundity due to knock-down of SaEcR and SaUSP in both the surviving aphids and their offspring, even after switching to aphid-susceptible wheat plants. Taken together, our results demonstrate that SaEcR and SaUSP are essential genes in aphid growth and development, and could be used as RNAi targets for wheat aphid control.

Links

PMC Free PDF

Authors+Show Affiliations

Yan T
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China. nmgyanting@sina.com.
Chen H
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China. chenchang1934@163.com.
Sun Y
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China. nuliba_hehe@163.com.
Yu X
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China. yuxiudao@163.com.
Xia L
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China. xialanqin@caas.cn.

MeSH

AnimalsAphidsFertilityGene Expression ProfilingGenes, InsectHerbivoryInsect ProteinsPhylogenyRNA InterferenceRNA, Double-StrandedReceptors, SteroidTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27983619

Citation

Yan, Ting, et al. "RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion Avenae F.) Affects Its Survival and Fecundity Upon Feeding On Wheat Plants." International Journal of Molecular Sciences, vol. 17, no. 12, 2016.
Yan T, Chen H, Sun Y, et al. RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants. Int J Mol Sci. 2016;17(12).
Yan, T., Chen, H., Sun, Y., Yu, X., & Xia, L. (2016). RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants. International Journal of Molecular Sciences, 17(12).
Yan T, et al. RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion Avenae F.) Affects Its Survival and Fecundity Upon Feeding On Wheat Plants. Int J Mol Sci. 2016 Dec 14;17(12) PubMed PMID: 27983619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants. AU - Yan,Ting, AU - Chen,Hongmei, AU - Sun,Yongwei, AU - Yu,Xiudao, AU - Xia,Lanqin, Y1 - 2016/12/14/ PY - 2016/09/04/received PY - 2016/12/05/revised PY - 2016/12/08/accepted PY - 2016/12/17/entrez PY - 2016/12/17/pubmed PY - 2017/4/4/medline KW - RNA interference (RNAi) KW - ecdysone receptor (EcR) KW - grain aphid (Sitobion avenae F.) KW - ultraspiracle protein (USP) JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 12 N2 - RNA interference (RNAi) has been widely used in functional genomics of insects and received intensive attention in the development of RNAi-based plants for insect control. Ecdysone receptor (EcR) and ultraspiracle protein (USP) play important roles in molting, metamorphosis, and reproduction of insects. EcR and USP orthologs and their function in grain aphid (Sitobion avenae F.) have not been documented yet. Here, RT-PCR, qRT-PCR, dsRNA feeding assay and aphid bioassay were employed to isolate EcR and USP orthologs in grain aphid, investigate their expression patterns, and evaluate the effect of RNAi on aphid survival and fecundity, and its persistence. The results indicated that SaEcR and SaUSP exhibited similar expression profiles at different developmental stages. Oral administration of dsRNAs of SaEcR and dsSaUSP significantly decreased the survival of aphids due to the down-regulation of these two genes, respectively. The silencing effect was persistent and transgenerational, as demonstrated by the reduced survival and fecundity due to knock-down of SaEcR and SaUSP in both the surviving aphids and their offspring, even after switching to aphid-susceptible wheat plants. Taken together, our results demonstrate that SaEcR and SaUSP are essential genes in aphid growth and development, and could be used as RNAi targets for wheat aphid control. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/27983619/RNA_Interference_of_the_Ecdysone_Receptor_Genes_EcR_and_USP_in_Grain_Aphid__Sitobion_avenae_F___Affects_Its_Survival_and_Fecundity_upon_Feeding_on_Wheat_Plants_ DB - PRIME DP - Unbound Medicine ER -