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Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera.
J Invertebr Pathol. 2017 01; 142:34-43.JI

Abstract

Western corn rootworm (WCR), Diabrotica virgifera virgifera, is one of the most significant pests of corn in the United States. Although transgenic solutions exist, increasing resistance concerns make the discovery of novel solutions essential. In order to find a novel protein with high activity and a new mode of action, a large microbial collection was surveyed for toxicity to WCR using in vitro bioassays. Cultures of strain ATX2024, identified as Chromobacterium piscinae, had very high activity against WCR larvae. The biological activity from the strain was purified using chromatographic techniques and fractions were tested against WCR larvae. Proteins in the final active fraction were identified by mass spectrometry and N-terminal sequencing and matched to the genome of ATX2024. A novel 58.9kDa protein, identified by this approach, was expressed in a recombinant expression system and found to have specific activity against WCR. Transgenic corn events containing this gene showed good protection against root damage by WCR, with average scores ranging between 0.01 and 0.04 on the Iowa State node injury scale. Sequence analysis did not reveal homology to any known insecticidal toxin, suggesting that this protein may act in a novel way to control WCR. The new WCR active protein is named GNIP1Aa, for Gram Negative Insecticidal Protein.

Authors+Show Affiliations

Bayer, Crop Science Division, Morrisville, NC, USA. Electronic address: kimberly.sampson@bayer.com.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.Bayer, Crop Science Division, Morrisville, NC, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27983944

Citation

Sampson, Kimberly, et al. "Discovery of a Novel Insecticidal Protein From Chromobacterium Piscinae, With Activity Against Western Corn Rootworm, Diabrotica Virgifera Virgifera." Journal of Invertebrate Pathology, vol. 142, 2017, pp. 34-43.
Sampson K, Zaitseva J, Stauffer M, et al. Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera. J Invertebr Pathol. 2017;142:34-43.
Sampson, K., Zaitseva, J., Stauffer, M., Vande Berg, B., Guo, R., Tomso, D., McNulty, B., Desai, N., & Balasubramanian, D. (2017). Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera. Journal of Invertebrate Pathology, 142, 34-43. https://doi.org/10.1016/j.jip.2016.10.004
Sampson K, et al. Discovery of a Novel Insecticidal Protein From Chromobacterium Piscinae, With Activity Against Western Corn Rootworm, Diabrotica Virgifera Virgifera. J Invertebr Pathol. 2017;142:34-43. PubMed PMID: 27983944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera. AU - Sampson,Kimberly, AU - Zaitseva,Jelena, AU - Stauffer,Maria, AU - Vande Berg,Brian, AU - Guo,Rong, AU - Tomso,Daniel, AU - McNulty,Brian, AU - Desai,Nalini, AU - Balasubramanian,Deepa, Y1 - 2016/10/28/ PY - 2016/03/30/received PY - 2016/09/19/revised PY - 2016/10/17/accepted PY - 2016/12/17/pubmed PY - 2017/9/28/medline PY - 2016/12/17/entrez KW - Chromobacterium piscinae KW - Diabrotica virgifera KW - Insecticidal protein toxin KW - Western corn rootworm SP - 34 EP - 43 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 142 N2 - Western corn rootworm (WCR), Diabrotica virgifera virgifera, is one of the most significant pests of corn in the United States. Although transgenic solutions exist, increasing resistance concerns make the discovery of novel solutions essential. In order to find a novel protein with high activity and a new mode of action, a large microbial collection was surveyed for toxicity to WCR using in vitro bioassays. Cultures of strain ATX2024, identified as Chromobacterium piscinae, had very high activity against WCR larvae. The biological activity from the strain was purified using chromatographic techniques and fractions were tested against WCR larvae. Proteins in the final active fraction were identified by mass spectrometry and N-terminal sequencing and matched to the genome of ATX2024. A novel 58.9kDa protein, identified by this approach, was expressed in a recombinant expression system and found to have specific activity against WCR. Transgenic corn events containing this gene showed good protection against root damage by WCR, with average scores ranging between 0.01 and 0.04 on the Iowa State node injury scale. Sequence analysis did not reveal homology to any known insecticidal toxin, suggesting that this protein may act in a novel way to control WCR. The new WCR active protein is named GNIP1Aa, for Gram Negative Insecticidal Protein. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/27983944/Discovery_of_a_novel_insecticidal_protein_from_Chromobacterium_piscinae_with_activity_against_Western_Corn_Rootworm_Diabrotica_virgifera_virgifera_ DB - PRIME DP - Unbound Medicine ER -