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The structure of aconitase.
Proteins. 1989; 5(4):289-312.P

Abstract

The crystal structure of the 80,000 Da Fe-S enzyme aconitase has been solved and refined at 2.1 A resolution. The protein contains four domains; the first three from the N-terminus are closely associated around the [3Fe-4S] cluster with all three cysteine ligands to the cluster being provided by the third domain. Association of the larger C-terminal domain with the first three domains creates an extensive cleft leading to the Fe-S cluster. Residues from all four domains contribute to the active site region, which is defined by the Fe-S cluster and a bound SO4(2-) ion. This region of the structure contains 4 Arg, 3 His, 3 Ser, 2 Asp, 1 Glu, 3 Asn, and 1 Gln residues, as well as several bound water molecules. Three of these side chains reside on a three-turn 3(10) helix in the first domain. The SO4(2-) ion is bound 9.3 A from the center of the [3Fe-4S] cluster by the side chains of 2 Arg and 1 Gln residues. Each of 3 His side chains in the putative active site is paired with Asp or Glu side chains.

Authors+Show Affiliations

Research Institute of Scripps Clinic, La Jolla, California 92037.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2798408

Citation

Robbins, A H., and C D. Stout. "The Structure of Aconitase." Proteins, vol. 5, no. 4, 1989, pp. 289-312.
Robbins AH, Stout CD. The structure of aconitase. Proteins. 1989;5(4):289-312.
Robbins, A. H., & Stout, C. D. (1989). The structure of aconitase. Proteins, 5(4), 289-312.
Robbins AH, Stout CD. The Structure of Aconitase. Proteins. 1989;5(4):289-312. PubMed PMID: 2798408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The structure of aconitase. AU - Robbins,A H, AU - Stout,C D, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 289 EP - 312 JF - Proteins JO - Proteins VL - 5 IS - 4 N2 - The crystal structure of the 80,000 Da Fe-S enzyme aconitase has been solved and refined at 2.1 A resolution. The protein contains four domains; the first three from the N-terminus are closely associated around the [3Fe-4S] cluster with all three cysteine ligands to the cluster being provided by the third domain. Association of the larger C-terminal domain with the first three domains creates an extensive cleft leading to the Fe-S cluster. Residues from all four domains contribute to the active site region, which is defined by the Fe-S cluster and a bound SO4(2-) ion. This region of the structure contains 4 Arg, 3 His, 3 Ser, 2 Asp, 1 Glu, 3 Asn, and 1 Gln residues, as well as several bound water molecules. Three of these side chains reside on a three-turn 3(10) helix in the first domain. The SO4(2-) ion is bound 9.3 A from the center of the [3Fe-4S] cluster by the side chains of 2 Arg and 1 Gln residues. Each of 3 His side chains in the putative active site is paired with Asp or Glu side chains. SN - 0887-3585 UR - https://www.unboundmedicine.com/medline/citation/2798408/The_structure_of_aconitase_ DB - PRIME DP - Unbound Medicine ER -