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Burkholderia humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis and the Fifth Member of the Burkholderia pseudomallei Complex.
Appl Environ Microbiol. 2017 03 01; 83(5)AE

Abstract

During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).IMPORTANCEBurkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. The genus Burkholderia consists of a diverse group of species, with the closest relatives of B. pseudomallei referred to as the B. pseudomallei complex. A proposed novel species, B. humptydooensis sp. nov., was isolated from a bore water sample from the Northern Territory in Australia. B. humptydooensis sp. nov. is phylogenetically distinct from B. pseudomallei and other members of the B. pseudomallei complex, making it the fifth member of this important group of bacteria.

Authors+Show Affiliations

Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Menzies School of Health Research, Charles Darwin University, and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia.Bundeswehr Institute of Microbiology, Munich, Germany.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Menzies School of Health Research, Charles Darwin University, and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Bundeswehr Institute of Microbiology, Munich, Germany.Bundeswehr Institute of Microbiology, Munich, Germany.Bundeswehr Institute of Microbiology, Munich, Germany.Bacterial Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.Center for Genome Sciences, USAMRIID, Fort Detrick, Maryland, USA.Center for Genome Sciences, USAMRIID, Fort Detrick, Maryland, USA.Center for Genome Sciences, USAMRIID, Fort Detrick, Maryland, USA.Center for Genome Sciences, USAMRIID, Fort Detrick, Maryland, USA.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA.Menzies School of Health Research, Charles Darwin University, and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia.Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA Paul.Keim@nau.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

27986727

Citation

Tuanyok, Apichai, et al. "Burkholderia Humptydooensis Sp. Nov., a New Species Related to Burkholderia Thailandensis and the Fifth Member of the Burkholderia Pseudomallei Complex." Applied and Environmental Microbiology, vol. 83, no. 5, 2017.
Tuanyok A, Mayo M, Scholz H, et al. Burkholderia humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis and the Fifth Member of the Burkholderia pseudomallei Complex. Appl Environ Microbiol. 2017;83(5).
Tuanyok, A., Mayo, M., Scholz, H., Hall, C. M., Allender, C. J., Kaestli, M., Ginther, J., Spring-Pearson, S., Bollig, M. C., Stone, J. K., Settles, E. W., Busch, J. D., Sidak-Loftis, L., Sahl, J. W., Thomas, A., Kreutzer, L., Georgi, E., Gee, J. E., Bowen, R. A., ... Keim, P. (2017). Burkholderia humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis and the Fifth Member of the Burkholderia pseudomallei Complex. Applied and Environmental Microbiology, 83(5). https://doi.org/10.1128/AEM.02802-16
Tuanyok A, et al. Burkholderia Humptydooensis Sp. Nov., a New Species Related to Burkholderia Thailandensis and the Fifth Member of the Burkholderia Pseudomallei Complex. Appl Environ Microbiol. 2017 03 1;83(5) PubMed PMID: 27986727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Burkholderia humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis and the Fifth Member of the Burkholderia pseudomallei Complex. AU - Tuanyok,Apichai, AU - Mayo,Mark, AU - Scholz,Holger, AU - Hall,Carina M, AU - Allender,Christopher J, AU - Kaestli,Mirjam, AU - Ginther,Jennifer, AU - Spring-Pearson,Senanu, AU - Bollig,Molly C, AU - Stone,Joshua K, AU - Settles,Erik W, AU - Busch,Joseph D, AU - Sidak-Loftis,Lindsay, AU - Sahl,Jason W, AU - Thomas,Astrid, AU - Kreutzer,Lisa, AU - Georgi,Enrico, AU - Gee,Jay E, AU - Bowen,Richard A, AU - Ladner,Jason T, AU - Lovett,Sean, AU - Koroleva,Galina, AU - Palacios,Gustavo, AU - Wagner,David M, AU - Currie,Bart J, AU - Keim,Paul, Y1 - 2017/02/15/ PY - 2016/10/07/received PY - 2016/12/02/accepted PY - 2016/12/18/pubmed PY - 2017/11/29/medline PY - 2016/12/18/entrez KW - Burkholderia humptydooensis sp. nov. KW - Burkholderia pseudomallei complex KW - MSMB43T JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 83 IS - 5 N2 - During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).IMPORTANCEBurkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. The genus Burkholderia consists of a diverse group of species, with the closest relatives of B. pseudomallei referred to as the B. pseudomallei complex. A proposed novel species, B. humptydooensis sp. nov., was isolated from a bore water sample from the Northern Territory in Australia. B. humptydooensis sp. nov. is phylogenetically distinct from B. pseudomallei and other members of the B. pseudomallei complex, making it the fifth member of this important group of bacteria. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/27986727/Burkholderia_humptydooensis_sp__nov__a_New_Species_Related_to_Burkholderia_thailandensis_and_the_Fifth_Member_of_the_Burkholderia_pseudomallei_Complex_ L2 - http://aem.asm.org/lookup/pmidlookup?view=long&pmid=27986727 DB - PRIME DP - Unbound Medicine ER -