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Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila: Effects on cholinergic and purinergic systems in liver tissue.
Fish Shellfish Immunol 2017; 61:1-8FS

Abstract

The aim of this study was to evaluate the effects of M. alternifolia essential oil used to treat silver catfish (Rhamdia quelen) experimentally infected by Aeromonas hydrophila on oxidative stress variables, and for the first time, on hepatic enzymes of the cholinergic and adenosinergic systems. For that, fish were divided into six groups (A-F), each containing seven animals. Groups A, B and C were composed of uninfected animals, while animals in groups D, E and F were intramuscularly inoculated with A. hydrophila. Groups B and E received a prophylactic bath with M. alternifolia essential oil (50 μL/L, diluted in ethanol) for seven days, while groups C and F were exposed to ethanol. After the prophylactic baths, groups D, E and F were inoculated with 100 μL of A. hydrophila solution (2.1 × 109 colony-forming unit). Two days after inoculation, the animals were euthanized and liver samples were collected. Infected animals (the group D) showed increased TBARS and protein carbonylation levels, while CAT, AChE and ADA activities decreased compared to uninfected animals (the group A). The prophylactic treatment with M. alternifolia essential oil (the group E) prevented the alterations caused by A. hydrophila, but it did not change AChE activity. Thus, the prophylactic treatment prevents damage caused by lipids and proteins, as well as alterations of the adenosinergic system, demonstrating that the anti-inflammatory effect of TTO is mediated by the adenosinergic pathway. In addition, TTO prophylactic treatment might be considered an important approach to prevent the hepatic damage caused by A. hydrophila.

Authors+Show Affiliations

Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: matheusd.biomed@yahoo.com.br.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, RS, Brazil.Laboratory of Phytochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Laboratory of Phytochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Animal Science Graduate Program, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: bbaldisserotto@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27988310

Citation

Baldissera, Matheus D., et al. "Melaleuca Alternifolia Essential Oil Enhances the Non-specific Immune System and Prevents Oxidative Damage in Rhamdia Quelen Experimentally Infected By Aeromonas Hydrophila: Effects On Cholinergic and Purinergic Systems in Liver Tissue." Fish & Shellfish Immunology, vol. 61, 2017, pp. 1-8.
Baldissera MD, Souza CF, Júnior GB, et al. Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila: Effects on cholinergic and purinergic systems in liver tissue. Fish Shellfish Immunol. 2017;61:1-8.
Baldissera, M. D., Souza, C. F., Júnior, G. B., de Vargas, A. C., Boligon, A. A., de Campos, M. M. A., ... Baldisserotto, B. (2017). Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila: Effects on cholinergic and purinergic systems in liver tissue. Fish & Shellfish Immunology, 61, pp. 1-8. doi:10.1016/j.fsi.2016.12.016.
Baldissera MD, et al. Melaleuca Alternifolia Essential Oil Enhances the Non-specific Immune System and Prevents Oxidative Damage in Rhamdia Quelen Experimentally Infected By Aeromonas Hydrophila: Effects On Cholinergic and Purinergic Systems in Liver Tissue. Fish Shellfish Immunol. 2017;61:1-8. PubMed PMID: 27988310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila: Effects on cholinergic and purinergic systems in liver tissue. AU - Baldissera,Matheus D, AU - Souza,Carine F, AU - Júnior,Guerino B, AU - de Vargas,Agueda C, AU - Boligon,Aline A, AU - de Campos,Marli M A, AU - Stefani,Lenita M, AU - Baldisserotto,Bernardo, Y1 - 2016/12/14/ PY - 2016/09/22/received PY - 2016/11/22/revised PY - 2016/12/13/accepted PY - 2016/12/19/pubmed PY - 2017/4/11/medline PY - 2016/12/19/entrez KW - Adenosine deaminase KW - Immune response KW - Inflammation KW - Tea tree oil (TTO) SP - 1 EP - 8 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 61 N2 - The aim of this study was to evaluate the effects of M. alternifolia essential oil used to treat silver catfish (Rhamdia quelen) experimentally infected by Aeromonas hydrophila on oxidative stress variables, and for the first time, on hepatic enzymes of the cholinergic and adenosinergic systems. For that, fish were divided into six groups (A-F), each containing seven animals. Groups A, B and C were composed of uninfected animals, while animals in groups D, E and F were intramuscularly inoculated with A. hydrophila. Groups B and E received a prophylactic bath with M. alternifolia essential oil (50 μL/L, diluted in ethanol) for seven days, while groups C and F were exposed to ethanol. After the prophylactic baths, groups D, E and F were inoculated with 100 μL of A. hydrophila solution (2.1 × 109 colony-forming unit). Two days after inoculation, the animals were euthanized and liver samples were collected. Infected animals (the group D) showed increased TBARS and protein carbonylation levels, while CAT, AChE and ADA activities decreased compared to uninfected animals (the group A). The prophylactic treatment with M. alternifolia essential oil (the group E) prevented the alterations caused by A. hydrophila, but it did not change AChE activity. Thus, the prophylactic treatment prevents damage caused by lipids and proteins, as well as alterations of the adenosinergic system, demonstrating that the anti-inflammatory effect of TTO is mediated by the adenosinergic pathway. In addition, TTO prophylactic treatment might be considered an important approach to prevent the hepatic damage caused by A. hydrophila. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/27988310/Melaleuca_alternifolia_essential_oil_enhances_the_non_specific_immune_system_and_prevents_oxidative_damage_in_Rhamdia_quelen_experimentally_infected_by_Aeromonas_hydrophila:_Effects_on_cholinergic_and_purinergic_systems_in_liver_tissue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(16)30778-1 DB - PRIME DP - Unbound Medicine ER -