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Anti-inflammatory effect of the six compounds isolated from Nauclea officinalis Pierrc ex Pitard, and molecular mechanism of strictosamide via suppressing the NF-κB and MAPK signaling pathway in LPS-induced RAW 264.7 macrophages.
J Ethnopharmacol. 2017 Jan 20; 196:66-74.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Nauclea officinalis Pierrc ex Pitard. is a Chinese medicinal herb that contains high level of alkaloids which is the most abundant and active constituent. Strictosamide isolated from Nauclea officinalis Pierrc ex Pitard. showed significant effects on inflammatory response, compared with pumiloside, 3-epi-pumiloside, vincosamide, 3α,5α-tetrahydrodeoxycordifoline lactam and naucleamide A-10-O-β-D-glucopyranoside of this plant.

AIM OF STUDY

we investigated the biological activities of the six compounds mentioned-above, and the underlying molecular mechanism exerted by the most potent one, strictosamide.

MATERIALS AND METHODS

The effects of strictosamide and other five compounds on the inhibitory activity of nitric oxide (NO) were screened by Griess test. The contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in media were detected by using Enzyme-linked immunosorbent (ELISA) kits. The effects on the mRNA expression of nitric oxide synthase (iNOS), TNF-α and IL-1β of strictosamide were further investigated by RT-qPCR. Western blot assay was conducted to illustrate the effects of strictosamide on iNOS and phosphorylation of p65, inhibitor of NF-κB (IκB)-α, IκB-kinase (IKK)-α as well as p-extracellular signal-regulated kinase (ERK), p-c-jun N-terminal kinase (JNK) and p-p38 in the protein levels.

RESULTS

Strictosamide potently suppressed the productions of NO, TNF-α and IL-1β in LPS-induced RAW 264.7 macrophages, and it dose-dependently alleviated the LPS-simulated protein level of iNOS as well as the mRNA expressions of iNOS, TNF-α and IL-1β. In addition, molecular data revealed that strictosamide markedly decreased the expressions of p-p65, p-IκBα and p-IKKα. Furthermore, strictosamide significantly attenuated LPS-induced the phosphorylation of p38, ERK and JNK.

CONCLUSIONS

At present study, the results indicated that the anti-inflammatory activity of strictosamide was associated with the restraint of NO, TNF-α and IL-1β via negative regulation of both NF-κB and mitogen-activated protein kinases (MAPKs) in LPS-induced RAW 264.7 cells.

Authors+Show Affiliations

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China; Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.Jiangsu Key Laboratory of Drug Design & Optimization, Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China; Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China. Electronic address: zfxcjq@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27989509

Citation

Li, DongYu, et al. "Anti-inflammatory Effect of the Six Compounds Isolated From Nauclea Officinalis Pierrc Ex Pitard, and Molecular Mechanism of Strictosamide Via Suppressing the NF-κB and MAPK Signaling Pathway in LPS-induced RAW 264.7 Macrophages." Journal of Ethnopharmacology, vol. 196, 2017, pp. 66-74.
Li D, Chen J, Ye J, et al. Anti-inflammatory effect of the six compounds isolated from Nauclea officinalis Pierrc ex Pitard, and molecular mechanism of strictosamide via suppressing the NF-κB and MAPK signaling pathway in LPS-induced RAW 264.7 macrophages. J Ethnopharmacol. 2017;196:66-74.
Li, D., Chen, J., Ye, J., Zhai, X., Song, J., Jiang, C., Wang, J., Zhang, H., Jia, X., & Zhu, F. (2017). Anti-inflammatory effect of the six compounds isolated from Nauclea officinalis Pierrc ex Pitard, and molecular mechanism of strictosamide via suppressing the NF-κB and MAPK signaling pathway in LPS-induced RAW 264.7 macrophages. Journal of Ethnopharmacology, 196, 66-74. https://doi.org/10.1016/j.jep.2016.12.007
Li D, et al. Anti-inflammatory Effect of the Six Compounds Isolated From Nauclea Officinalis Pierrc Ex Pitard, and Molecular Mechanism of Strictosamide Via Suppressing the NF-κB and MAPK Signaling Pathway in LPS-induced RAW 264.7 Macrophages. J Ethnopharmacol. 2017 Jan 20;196:66-74. PubMed PMID: 27989509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of the six compounds isolated from Nauclea officinalis Pierrc ex Pitard, and molecular mechanism of strictosamide via suppressing the NF-κB and MAPK signaling pathway in LPS-induced RAW 264.7 macrophages. AU - Li,DongYu, AU - Chen,JiaQuan, AU - Ye,JiQing, AU - Zhai,XiaoTing, AU - Song,Jie, AU - Jiang,CuiHua, AU - Wang,Jing, AU - Zhang,Hao, AU - Jia,XiaoBin, AU - Zhu,FenXia, Y1 - 2016/12/15/ PY - 2016/09/25/received PY - 2016/12/05/revised PY - 2016/12/07/accepted PY - 2016/12/19/pubmed PY - 2017/7/14/medline PY - 2016/12/20/entrez KW - Anti-inflammation KW - DMSO (PubChem CID: 679) KW - MAPK KW - NF-κB KW - NaNO(2) (PubChem CID: 23668193) KW - Pro-inflammatory cytokines KW - Pumiloside (ChemSpider ID: 8521772) KW - Strictosamide KW - Strictosamide (PubChem CID: 10345799) KW - Vincosamide (PubChem CID: 10163855) SP - 66 EP - 74 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 196 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Nauclea officinalis Pierrc ex Pitard. is a Chinese medicinal herb that contains high level of alkaloids which is the most abundant and active constituent. Strictosamide isolated from Nauclea officinalis Pierrc ex Pitard. showed significant effects on inflammatory response, compared with pumiloside, 3-epi-pumiloside, vincosamide, 3α,5α-tetrahydrodeoxycordifoline lactam and naucleamide A-10-O-β-D-glucopyranoside of this plant. AIM OF STUDY: we investigated the biological activities of the six compounds mentioned-above, and the underlying molecular mechanism exerted by the most potent one, strictosamide. MATERIALS AND METHODS: The effects of strictosamide and other five compounds on the inhibitory activity of nitric oxide (NO) were screened by Griess test. The contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in media were detected by using Enzyme-linked immunosorbent (ELISA) kits. The effects on the mRNA expression of nitric oxide synthase (iNOS), TNF-α and IL-1β of strictosamide were further investigated by RT-qPCR. Western blot assay was conducted to illustrate the effects of strictosamide on iNOS and phosphorylation of p65, inhibitor of NF-κB (IκB)-α, IκB-kinase (IKK)-α as well as p-extracellular signal-regulated kinase (ERK), p-c-jun N-terminal kinase (JNK) and p-p38 in the protein levels. RESULTS: Strictosamide potently suppressed the productions of NO, TNF-α and IL-1β in LPS-induced RAW 264.7 macrophages, and it dose-dependently alleviated the LPS-simulated protein level of iNOS as well as the mRNA expressions of iNOS, TNF-α and IL-1β. In addition, molecular data revealed that strictosamide markedly decreased the expressions of p-p65, p-IκBα and p-IKKα. Furthermore, strictosamide significantly attenuated LPS-induced the phosphorylation of p38, ERK and JNK. CONCLUSIONS: At present study, the results indicated that the anti-inflammatory activity of strictosamide was associated with the restraint of NO, TNF-α and IL-1β via negative regulation of both NF-κB and mitogen-activated protein kinases (MAPKs) in LPS-induced RAW 264.7 cells. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27989509/Anti_inflammatory_effect_of_the_six_compounds_isolated_from_Nauclea_officinalis_Pierrc_ex_Pitard_and_molecular_mechanism_of_strictosamide_via_suppressing_the_NF_κB_and_MAPK_signaling_pathway_in_LPS_induced_RAW_264_7_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30996-5 DB - PRIME DP - Unbound Medicine ER -