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In vitro and in silico investigations of the binary-mixture toxicity of phthalate esters and cadmium (II) to Vibrio qinghaiensis sp.-Q67.
Sci Total Environ. 2017 Feb 15; 580:1078-1084.ST

Abstract

Phthalate esters (PAEs) are widely used as plasticizers and have become one of the emerging contaminants with an increasing public concern. The residues of PAEs frequently co-exist with heavy metals such as cadmium (Cd) in waters; however, their joint ecotoxicity remains largely unknown. We herein investigated the single and joint toxicity of commonly used PAEs and Cd using freshwater luminescent bacteria Vibrio qinghaiensis sp.-Q67. The median effective concentration (EC50) of benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), diethyl phthalate (DEP), dimethyl phthalate (DMP), diisooctyl phthalate (DIOP) and di-n-octyl phthalate (DOP) were determined to be in the range from 134.4mg/L to as high as 1000mg/L, indicating very weak toxicity to Vibrio qinghaiensis sp.-Q67. The toxicity of single PAEs showed a significant linear relationship with Log Kow, indicating the dependence of the elevated toxicity on the increasing hydrophilicity. The toxicity of binary mixture of PAEs was further evaluated in silico using the independent action (IA) model and concentration addition (CA) model. DMP-DEP, DEP-DBP or DMP-DBP exhibited antagonistic effects with the toxic unit value higher than 1.2. The CA and IA models poorly predicted the joint toxicity of DMP-DEP, DEP-DBP or DMP-DBP. The joint toxicity of the binary mixtures of DMP, DEP or DBP with Cd was simple additive as predicted by the CA and IA models. Our results indicated the potentially higher risk of PAEs in the presence of Cd, emphasizing the importance of determining the impact of their joint effects on aquatic organisms. The integrated in vitro and in silico methods employed in this study will be beneficial to study the joint toxicity and better assess the aquatic ecological risk of PAEs.

Authors+Show Affiliations

College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 316022, China.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 316022, China.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.Zhejiang Province Environmental Monitoring Center, Hangzhou 310005, China.Department of Biological and Environmental Sciences, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058, USA.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 316022, China. Electronic address: shulin@zju.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27993475

Citation

Ding, Keke, et al. "In Vitro and in Silico Investigations of the Binary-mixture Toxicity of Phthalate Esters and Cadmium (II) to Vibrio Qinghaiensis Sp.-Q67." The Science of the Total Environment, vol. 580, 2017, pp. 1078-1084.
Ding K, Lu L, Wang J, et al. In vitro and in silico investigations of the binary-mixture toxicity of phthalate esters and cadmium (II) to Vibrio qinghaiensis sp.-Q67. Sci Total Environ. 2017;580:1078-1084.
Ding, K., Lu, L., Wang, J., Wang, J., Zhou, M., Zheng, C., Liu, J., Zhang, C., & Zhuang, S. (2017). In vitro and in silico investigations of the binary-mixture toxicity of phthalate esters and cadmium (II) to Vibrio qinghaiensis sp.-Q67. The Science of the Total Environment, 580, 1078-1084. https://doi.org/10.1016/j.scitotenv.2016.12.062
Ding K, et al. In Vitro and in Silico Investigations of the Binary-mixture Toxicity of Phthalate Esters and Cadmium (II) to Vibrio Qinghaiensis Sp.-Q67. Sci Total Environ. 2017 Feb 15;580:1078-1084. PubMed PMID: 27993475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in silico investigations of the binary-mixture toxicity of phthalate esters and cadmium (II) to Vibrio qinghaiensis sp.-Q67. AU - Ding,Keke, AU - Lu,Liping, AU - Wang,Jiaying, AU - Wang,Jingpeng, AU - Zhou,Minqiang, AU - Zheng,Cunwu, AU - Liu,Jinsong, AU - Zhang,Chunlong, AU - Zhuang,Shulin, Y1 - 2016/12/16/ PY - 2016/10/28/received PY - 2016/12/09/revised PY - 2016/12/09/accepted PY - 2016/12/21/pubmed PY - 2018/6/8/medline PY - 2016/12/21/entrez KW - Acute toxicity KW - Concentration addition KW - Independent action KW - Phthalate esters KW - Simple addition SP - 1078 EP - 1084 JF - The Science of the total environment JO - Sci Total Environ VL - 580 N2 - Phthalate esters (PAEs) are widely used as plasticizers and have become one of the emerging contaminants with an increasing public concern. The residues of PAEs frequently co-exist with heavy metals such as cadmium (Cd) in waters; however, their joint ecotoxicity remains largely unknown. We herein investigated the single and joint toxicity of commonly used PAEs and Cd using freshwater luminescent bacteria Vibrio qinghaiensis sp.-Q67. The median effective concentration (EC50) of benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), diethyl phthalate (DEP), dimethyl phthalate (DMP), diisooctyl phthalate (DIOP) and di-n-octyl phthalate (DOP) were determined to be in the range from 134.4mg/L to as high as 1000mg/L, indicating very weak toxicity to Vibrio qinghaiensis sp.-Q67. The toxicity of single PAEs showed a significant linear relationship with Log Kow, indicating the dependence of the elevated toxicity on the increasing hydrophilicity. The toxicity of binary mixture of PAEs was further evaluated in silico using the independent action (IA) model and concentration addition (CA) model. DMP-DEP, DEP-DBP or DMP-DBP exhibited antagonistic effects with the toxic unit value higher than 1.2. The CA and IA models poorly predicted the joint toxicity of DMP-DEP, DEP-DBP or DMP-DBP. The joint toxicity of the binary mixtures of DMP, DEP or DBP with Cd was simple additive as predicted by the CA and IA models. Our results indicated the potentially higher risk of PAEs in the presence of Cd, emphasizing the importance of determining the impact of their joint effects on aquatic organisms. The integrated in vitro and in silico methods employed in this study will be beneficial to study the joint toxicity and better assess the aquatic ecological risk of PAEs. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/27993475/In_vitro_and_in_silico_investigations_of_the_binary_mixture_toxicity_of_phthalate_esters_and_cadmium__II__to_Vibrio_qinghaiensis_sp__Q67_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(16)32749-8 DB - PRIME DP - Unbound Medicine ER -