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[Interaction of polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T with Helicobacter pylori infection in esophageal squamous cell carcinoma].
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Dec 20; 36(12):1638-1649.NF

Abstract

OBJECTIVE

To investigate the interaction of polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T with helicobacter pylori (H. pylori) infection in esophageal squamous cell carcinoma (ESCC) .

METHODS

A total of 200 cases of LSCC of Broder grade I, 200 of Broder grade II and of grade III were enrolled in this study with 200 healthy individuals as the control group. The genetic polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T were analyzed using PCR-RFLP in peripheral blood leukocytes. 14C-urea breath test (14C-UBT) was used to test 14C disntegration per minute (DPM) for evaluating the infection status of H. pylori. An unconditional logistic regression model was used to analyze the interaction of nucleotide polymorphisms and H. pylori infection.

RESULTS

The risk of ESCC significantly increased in subjects with -C34G (CG), -C34G(GG), -C242T (CT), and -C242T (TT) genotypes. Combined analysis of the polymorphisms showed that the subjects carrying -C34G (GG)/ -C242T (TT) had a high risk of ESCC, and a positive interaction was found between -C34G (GG) and -C242T (TT) in increasing the risk of ESCC. Positive interactions in the pathogenesis of ESCC were also found between -C34G (CG) and -C242T (TT), between -C34G (CG) and -C242T (CT), and between -C34G (GG) and -C242T (CT) (γ>1). The risk of ESCC significantly increased in subjects with H. pylori infection, which showed positive interactions with -C34G (CG), -C34G (GG), -C242T (CT) and -C242T (TT) in increasing the risk of ESCC (γ>1).

CONCLUSION

Individuals carrying -C34G(CG), -C34G(GG), -C242T (CT) and -C242T (TT) genotypes have a high risk of developing ESCC, and these genotypes interact with H. pylori infection in the pathogenesis of LSCC, suggesting the importance of eradicating H. pylori for prevention of ESCC.

Authors+Show Affiliations

Department of Gastroenterology, First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China. E-mail: nn21882001@aliyun.com.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

27998858

Citation

Zhang, Chao-Xian, et al. "[Interaction of Polymorphisms of PPAR-γ2 Gene -C34G and NADPH Oxidase Subunit P22phox Gene -C242T With Helicobacter Pylori Infection in Esophageal Squamous Cell Carcinoma]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 36, no. 12, 2016, pp. 1638-1649.
Zhang CX, Guo LK, Zhang LL, et al. [Interaction of polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T with Helicobacter pylori infection in esophageal squamous cell carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao. 2016;36(12):1638-1649.
Zhang, C. X., Guo, L. K., Zhang, L. L., & Han, Y. (2016). [Interaction of polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T with Helicobacter pylori infection in esophageal squamous cell carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 36(12), 1638-1649.
Zhang CX, et al. [Interaction of Polymorphisms of PPAR-γ2 Gene -C34G and NADPH Oxidase Subunit P22phox Gene -C242T With Helicobacter Pylori Infection in Esophageal Squamous Cell Carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao. 2016 Dec 20;36(12):1638-1649. PubMed PMID: 27998858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Interaction of polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T with Helicobacter pylori infection in esophageal squamous cell carcinoma]. AU - Zhang,Chao-Xian, AU - Guo,Li-Ke, AU - Zhang,Li-Li, AU - Han,Yu, PY - 2016/12/22/entrez PY - 2016/12/22/pubmed PY - 2018/4/14/medline SP - 1638 EP - 1649 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 36 IS - 12 N2 - OBJECTIVE: To investigate the interaction of polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T with helicobacter pylori (H. pylori) infection in esophageal squamous cell carcinoma (ESCC) . METHODS: A total of 200 cases of LSCC of Broder grade I, 200 of Broder grade II and of grade III were enrolled in this study with 200 healthy individuals as the control group. The genetic polymorphisms of PPAR-γ2 gene -C34G and NADPH oxidase subunit p22phox gene -C242T were analyzed using PCR-RFLP in peripheral blood leukocytes. 14C-urea breath test (14C-UBT) was used to test 14C disntegration per minute (DPM) for evaluating the infection status of H. pylori. An unconditional logistic regression model was used to analyze the interaction of nucleotide polymorphisms and H. pylori infection. RESULTS: The risk of ESCC significantly increased in subjects with -C34G (CG), -C34G(GG), -C242T (CT), and -C242T (TT) genotypes. Combined analysis of the polymorphisms showed that the subjects carrying -C34G (GG)/ -C242T (TT) had a high risk of ESCC, and a positive interaction was found between -C34G (GG) and -C242T (TT) in increasing the risk of ESCC. Positive interactions in the pathogenesis of ESCC were also found between -C34G (CG) and -C242T (TT), between -C34G (CG) and -C242T (CT), and between -C34G (GG) and -C242T (CT) (γ>1). The risk of ESCC significantly increased in subjects with H. pylori infection, which showed positive interactions with -C34G (CG), -C34G (GG), -C242T (CT) and -C242T (TT) in increasing the risk of ESCC (γ>1). CONCLUSION: Individuals carrying -C34G(CG), -C34G(GG), -C242T (CT) and -C242T (TT) genotypes have a high risk of developing ESCC, and these genotypes interact with H. pylori infection in the pathogenesis of LSCC, suggesting the importance of eradicating H. pylori for prevention of ESCC. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/27998858/[Interaction_of_polymorphisms_of_PPAR_γ2_gene__C34G_and_NADPH_oxidase_subunit_p22phox_gene__C242T_with_Helicobacter_pylori_infection_in_esophageal_squamous_cell_carcinoma]_ L2 - https://medlineplus.gov/esophagealcancer.html DB - PRIME DP - Unbound Medicine ER -